4.1.2.47: (S)-hydroxynitrile lyase

This is an abbreviated version!
For detailed information about (S)-hydroxynitrile lyase, go to the full flat file.

Reaction

Synonyms

(R)-LuHNL, (R)-Oxynitrilase, (S)-cyanohydrin producing hydroxynitrile lyase, (S)-Hb-HNL, (S)-HbHNL, (S)-HNL, (S)-Hydroxynitrile lyase, (S)-Me-HNL, (S)-MeHNL, (S)-Oxynitrilase, (S)-selective HNL, (S)-selective hydroxynitrile lyase, Acetone cyanohydrin lyase, ACL, alpha-Hydroxynitrile lyase, EC 4.1.2.37, EC 4.1.2.39, Hb-HNL, HbHNL, HNL, HNL1, Hydroxynitrile lyase, LuHNL, MeHNKL, MeHNL, mut-HNL1, Oxynitrilase, S-HnL, S-hydroxynitrile lyase, S-selective HnL, S-selective hydroxynitrile lyase, S-stereoselective HNL

ECTree

     4 Lyases

         4.1 Carbon-carbon lyases

             4.1.2 Aldehyde-lyases

                4.1.2.47 (S)-hydroxynitrile lyase

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Results	in table
3	Activating Compound
3	AA Sequence
27	Application
33	Cloned(Commentary)
3	Cofactor
16	Crystallization (Commentary)
2	Expression
1	General Information
8	General Stability
50	Inhibitors
77	kcat/KM [mM/s]
2	Ki Value [mM]
135	KM Value [mM]
2	Localization
23	Molecular Weight [Da]
23	Natural Substrates/ Products (Substrates)
4	Organic Solvent Stability
94	Organism
2	Oxidation Stability
3	Pathway
12	PDB ID
23	pH Optimum
4	pH Range
17	pH Stability
2	pI Value
2	Posttranslational Modification
99	Protein Variants
26	Purification (Commentary)
3	Reaction
6	Reaction Type
91	Reference
20	Source Tissue
76	Specific Activity [micromol/min/mg]
1	Storage Stability
368	Substrates and Products (Substrate)
14	Subunits
134	Synonyms
1	Systematic Name
7	Temperature Optimum [°C]
3	Temperature Range [°C]
17	Temperature Stability [°C]
100	Turnover Number [1/s]

General Stability

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General Stability on EC 4.1.2.47 - (S)-hydroxynitrile lyase

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GENERAL STABILITY

ORGANISM

UNIPROT

LITERATURE

half-life at pH 5 and 20°C is halved at a concentration of 2 mM benzaldehyde and 30 mM HCN, stability decreases drastically with increasing concentrations of benzaldehyde

Hevea brasiliensis

-

651011

half-life increases linearly with enzyme concentration in the aqueous buffer as well as in a two-phase system of buffer and dibutyl ether

Hevea brasiliensis

-

651011

HNL is stabilized by entrapment in aggregates of Escherichia coli cell proteins

Manihot esculenta

P52705

704762

influence of different organic solvents and solvent mixtures on the stability in two-phase systems of aqueous buffer and organic solvent

Hevea brasiliensis

-

651011

the enzyme is stabilized in crude cell extract

Manihot esculenta

P52705

704762

the enzyme shows especially high stability in mixtures of methyl-tert-butylether and hexane, 40:60 and 25:75

Hevea brasiliensis

-

651011

the immobilized enzyme is successfully used for more than 20 repeated batches with no loss of conversion rate or enantioselectivity

Manihot esculenta

P52705

691373

the use of solvent mixtures with methyl-tert-butylether leads to better stability than the use of pure solvents

Hevea brasiliensis

-

651011