4.1.2.47: (S)-hydroxynitrile lyase
This is an abbreviated version!
For detailed information about (S)-hydroxynitrile lyase, go to the full flat file.
Word Map on EC 4.1.2.47
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4.1.2.47
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esculenta
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manihot
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brasiliensis
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hevea
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synthesis
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cassava
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ketone
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hcn
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lyases
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benzaldehyde
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s-mandelonitrile
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rubber
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athnl
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crantz
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hydrocyanic
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s-cyanohydrins
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r-selective
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arylacetonitrilase
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s-enantiomer
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cyanogenesis
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montanum
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s-mandelic
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pharmacology
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agriculture
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analysis
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drug development
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food industry
- 4.1.2.47
- esculenta
- manihot
- brasiliensis
- hevea
- synthesis
- cassava
- ketone
- hcn
- lyases
- benzaldehyde
-
s-mandelonitrile
- rubber
- athnl
- crantz
-
hydrocyanic
-
s-cyanohydrins
-
r-selective
- arylacetonitrilase
-
s-enantiomer
-
cyanogenesis
- montanum
-
s-mandelic
- pharmacology
- agriculture
- analysis
- drug development
- food industry
Reaction
Synonyms
(R)-LuHNL, (R)-Oxynitrilase, (S)-cyanohydrin producing hydroxynitrile lyase, (S)-Hb-HNL, (S)-HbHNL, (S)-HNL, (S)-Hydroxynitrile lyase, (S)-Me-HNL, (S)-MeHNL, (S)-Oxynitrilase, (S)-selective HNL, (S)-selective hydroxynitrile lyase, Acetone cyanohydrin lyase, ACL, alpha-Hydroxynitrile lyase, EC 4.1.2.37, EC 4.1.2.39, Hb-HNL, HbHNL, HNL, HNL1, Hydroxynitrile lyase, LuHNL, MeHNKL, MeHNL, mut-HNL1, Oxynitrilase, S-HnL, S-hydroxynitrile lyase, S-selective HnL, S-selective hydroxynitrile lyase, S-stereoselective HNL
ECTree
4.1.2.47 (S)-hydroxynitrile lyaseAdvanced search results
results (
results (KM Value
KM Value on EC 4.1.2.47 - (S)-hydroxynitrile lyase
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KM VALUE [mM] 
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
IMAGE
0.61
(R)-mandelonitrile
pH 4.23, 25°C, mutant enzyme H103C/N156D
1.49
(R)-mandelonitrile
pH 4.23, 25°C, mutant enzyme H103C/N156G
2.6
(S)-2-nitro-1-phenylethanol
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in 50 mM phosphate buffer pH 6.0, at 25°C
2.6
(S)-2-nitro-1-phenylethanol
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in 50 mM phosphate buffer pH 6.0, at 22°C
0.47
(S)-mandelonitrile
pH 4.23, 25°C, mutant enzyme H103C/N156D
0.49
(S)-mandelonitrile
pH 4.23, 25°C, mutant enzyme H103C/N156G
1.2
(S)-mandelonitrile
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in 50 mM citrate buffer (pH 5.0), temperature not specified in the publication
1.4
(S)-mandelonitrile
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in 50 mM citrate buffer (pH 5.0), temperature not specified in the publication
1.86
(S)-mandelonitrile
mutant enzyme H103M, in 100 mM citrate buffer pH above 6.0, temperature not specified in the publication
2.5 - 5
(S)-mandelonitrile
mutant enzyme H103L, in 100 mM citrate buffer pH above 6.0, temperature not specified in the publication
5.17
(S)-mandelonitrile
wild type enzyme, in 100 mM citrate buffer pH above 6.0, temperature not specified in the publication
5.54
(S)-mandelonitrile
mutant enzyme K176P/K199P/K224P, in 100 mM citrate buffer pH above 6.0, temperature not specified in the publication
6.98
(S)-mandelonitrile
mutant enzyme K176P, in 100 mM citrate buffer pH above 6.0, temperature not specified in the publication
150
2-hydroxy-2-methylpropanenitrile
pH 5.2, 23°C, mutant enzyme W128A
0.4
2-nitro-1-phenylethanol
mutant enzyme F125T/L146M, pH and temperature not specified in the publication
0.7
2-nitro-1-phenylethanol
mutant enzyme F125T/Y133F, pH and temperature not specified in the publication
1.4
2-nitro-1-phenylethanol
mutant enzyme V106F, pH and temperature not specified in the publication
1.6
2-nitro-1-phenylethanol
mutant enzyme V106F/L121Y, pH and temperature not specified in the publication
1.8
2-nitro-1-phenylethanol
mutant enzyme V106F/F125T, pH and temperature not specified in the publication
5
2-nitro-1-phenylethanol
mutant enzyme C81L, pH and temperature not specified in the publication
5
2-nitro-1-phenylethanol
mutant enzyme F125T, pH and temperature not specified in the publication
5
2-nitro-1-phenylethanol
mutant enzyme L121Y, pH and temperature not specified in the publication
5
2-nitro-1-phenylethanol
mutant enzyme L121Y/F125T/L146M, pH and temperature not specified in the publication
5
2-nitro-1-phenylethanol
mutant enzyme L121Y/F125T/Y133F, pH and temperature not specified in the publication
5
2-nitro-1-phenylethanol
mutant enzyme L121Y/L146M, pH and temperature not specified in the publication
5
2-nitro-1-phenylethanol
mutant enzyme L146M, pH and temperature not specified in the publication
5
2-nitro-1-phenylethanol
mutant enzyme V106F/L121Y/F125T, pH and temperature not specified in the publication
5
2-nitro-1-phenylethanol
mutant enzyme V106F/L146M, pH and temperature not specified in the publication
6.1
2-nitro-1-phenylethanol
wild type enzyme, pH and temperature not specified in the publication
7.1
2-nitro-1-phenylethanol
mutant enzyme Y133F, pH and temperature not specified in the publication
2 - 3
benzaldehyde
wild type enzyme, in 100 mM citrate buffer pH 5.0, temperature not specified in the publication
12.3
benzaldehyde
mutant enzyme H103Y, in 100 mM citrate buffer pH 5.0, temperature not specified in the publication
13.4
benzaldehyde
mutant enzyme H103L, in 100 mM citrate buffer pH 5.0, temperature not specified in the publication
21.6
benzaldehyde
mutant enzyme K176P, in 100 mM citrate buffer pH 5.0, temperature not specified in the publication
27
benzaldehyde
mutant enzyme K176P/K199P/K224P, in 100 mM citrate buffer pH 5.0, temperature not specified in the publication
27.9
benzaldehyde
mutant enzyme H103M, in 100 mM citrate buffer pH 5.0, temperature not specified in the publication
0.6
mandelonitrile
mutant enzyme L152F, pH and temperature not specified in the publication
0.7
mandelonitrile
mutant enzyme I209G, pH and temperature not specified in the publication
0.8
mandelonitrile
mutant enzyme I209A, pH and temperature not specified in the publication
1.4
mandelonitrile
mutant enzyme V106F/L121Y, pH and temperature not specified in the publication
1.5
mandelonitrile
mutant enzyme F125T/L146M, pH and temperature not specified in the publication
2
mandelonitrile
mutant enzyme F125T, pH and temperature not specified in the publication
2
mandelonitrile
mutant enzyme V106F/L121Y/F125T, pH and temperature not specified in the publication
2.1
mandelonitrile
mutant enzyme F125T/Y133F, pH and temperature not specified in the publication
2.7
mandelonitrile
mutant enzyme L121Y, pH and temperature not specified in the publication
3.1
mandelonitrile
mutant enzyme L121Y/F125T, pH and temperature not specified in the publication
3.1
mandelonitrile
mutant enzyme Y133F, pH and temperature not specified in the publication
3.3
mandelonitrile
wild type enzyme, pH and temperature not specified in the publication
3.6
mandelonitrile
mutant enzyme L121Y/F125T/L146M, pH and temperature not specified in the publication
3.7
mandelonitrile
mutant enzyme L146M, pH and temperature not specified in the publication
5.3
mandelonitrile
mutant enzyme V106F, pH and temperature not specified in the publication
6.7
mandelonitrile
mutant enzyme L121Y/L146M, pH and temperature not specified in the publication
8.6
mandelonitrile
mutant enzyme F210I, pH and temperature not specified in the publication
8.6
mandelonitrile
mutant enzyme L148F, pH and temperature not specified in the publication
9.4
mandelonitrile
mutant enzyme C81L, pH and temperature not specified in the publication
0.4
rac-2-nitro-1-phenylethanol
pH 5.0, temperature not specified in the publication, mutant enzyme F125T/L146M
0.7
rac-2-nitro-1-phenylethanol
pH 5.0, temperature not specified in the publication, mutant enzyme F125T/Y133F
1.4
rac-2-nitro-1-phenylethanol
pH 5.0, temperature not specified in the publication, mutant enzyme V106F
1.6
rac-2-nitro-1-phenylethanol
pH 5.0, temperature not specified in the publication, mutant enzyme V106F/L121Y
1.8
rac-2-nitro-1-phenylethanol
pH 5.0, temperature not specified in the publication, mutant enzyme V106F/F125T
6.1
rac-2-nitro-1-phenylethanol
pH 5.0, temperature not specified in the publication, wild-type enzyme
7.1
rac-2-nitro-1-phenylethanol
pH 5.0, temperature not specified in the publication, mutant enzyme Y133F
0.6
rac-mandelonitrile
pH 5.0, temperature not specified in the publication, mutant enzyme L152F
0.7
rac-mandelonitrile
pH 5.0, temperature not specified in the publication, mutant enzyme I209G
0.8
rac-mandelonitrile
pH 5.0, temperature not specified in the publication, mutant enzyme I209A
1.4
rac-mandelonitrile
pH 5.0, temperature not specified in the publication, mutant enzyme V106F/L121Y
1.5
rac-mandelonitrile
pH 5.0, temperature not specified in the publication, mutant enzyme F125T/L146M
1.5
rac-mandelonitrile
pH 5.0, temperature not specified in the publication, mutant enzyme L121F
2
rac-mandelonitrile
pH 5.0, temperature not specified in the publication, mutant enzyme F125T
2
rac-mandelonitrile
pH 5.0, temperature not specified in the publication, mutant enzyme V106F/L121Y/F125T
2.1
rac-mandelonitrile
pH 5.0, temperature not specified in the publication, mutant enzyme F125T/Y133F
2.7
rac-mandelonitrile
pH 5.0, temperature not specified in the publication, mutant enzyme L121T
2.7
rac-mandelonitrile
pH 5.0, temperature not specified in the publication, mutant enzyme L121Y
2.8
rac-mandelonitrile
pH 5.0, temperature not specified in the publication, mutant enzyme L121A
3.1
rac-mandelonitrile
pH 5.0, temperature not specified in the publication, mutant enzyme L121Y/F125T
3.1
rac-mandelonitrile
pH 5.0, temperature not specified in the publication, mutant enzyme Y133F
3.3
rac-mandelonitrile
pH 5.0, temperature not specified in the publication, wild-type enzyme
3.6
rac-mandelonitrile
pH 5.0, temperature not specified in the publication, mutant enzyme L121Y/F125T/L146M
3.7
rac-mandelonitrile
pH 5.0, temperature not specified in the publication, mutant enzyme L121V
3.7
rac-mandelonitrile
pH 5.0, temperature not specified in the publication, mutant enzyme L146M
4
rac-mandelonitrile
pH 5.0, temperature not specified in the publication, mutant enzyme L121M
4.9
rac-mandelonitrile
pH 5.0, temperature not specified in the publication, mutant enzyme L121I
5.3
rac-mandelonitrile
pH 5.0, temperature not specified in the publication, mutant enzyme V106F
5.6
rac-mandelonitrile
pH 5.0, temperature not specified in the publication, mutant enzyme L121W
5.7
rac-mandelonitrile
pH 5.0, temperature not specified in the publication, mutant enzyme L121Q
6.6
rac-mandelonitrile
pH 5.0, temperature not specified in the publication, mutant enzyme L121S
6.7
rac-mandelonitrile
pH 5.0, temperature not specified in the publication, mutant enzyme L121Y/L146M
8
rac-mandelonitrile
pH 5.0, temperature not specified in the publication, mutant enzyme L121H
8.1
rac-mandelonitrile
pH 5.0, temperature not specified in the publication, mutant enzyme L121C
8.6
rac-mandelonitrile
pH 5.0, temperature not specified in the publication, mutant enzyme F210I
8.6
rac-mandelonitrile
pH 5.0, temperature not specified in the publication, mutant enzyme L148F
9.4
rac-mandelonitrile
pH 5.0, temperature not specified in the publication, mutant enzyme C81L
additional information
additional information
a kinetic investigation based on the rate curve method
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additional information
additional information
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a kinetic investigation based on the rate curve method
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additional information
additional information
wild-type enzyme shows very low activity with 4-hydroxymandelonitrile, activity of mutant enzyme W128A is increased 450fold compared to wild-type value (Km-value for mutant enzyme W128A is 0.625 mM)
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additional information
additional information
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wild-type enzyme shows very low activity with 4-hydroxymandelonitrile, activity of mutant enzyme W128A is increased 450fold compared to wild-type value (Km-value for mutant enzyme W128A is 0.625 mM)
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