4.1.2.47: (S)-hydroxynitrile lyase
This is an abbreviated version!
For detailed information about (S)-hydroxynitrile lyase, go to the full flat file.
Word Map on EC 4.1.2.47
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4.1.2.47
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esculenta
-
manihot
-
brasiliensis
-
hevea
-
synthesis
-
cassava
-
ketone
-
hcn
-
lyases
-
benzaldehyde
-
s-mandelonitrile
-
rubber
-
athnl
-
crantz
-
hydrocyanic
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s-cyanohydrins
-
r-selective
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arylacetonitrilase
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s-enantiomer
-
cyanogenesis
-
montanum
-
s-mandelic
-
pharmacology
-
agriculture
-
analysis
-
drug development
-
food industry
- 4.1.2.47
- esculenta
- manihot
- brasiliensis
- hevea
- synthesis
- cassava
- ketone
- hcn
- lyases
- benzaldehyde
-
s-mandelonitrile
- rubber
- athnl
- crantz
-
hydrocyanic
-
s-cyanohydrins
-
r-selective
- arylacetonitrilase
-
s-enantiomer
-
cyanogenesis
- montanum
-
s-mandelic
- pharmacology
- agriculture
- analysis
- drug development
- food industry
Reaction
Synonyms
(R)-LuHNL, (R)-Oxynitrilase, (S)-cyanohydrin producing hydroxynitrile lyase, (S)-Hb-HNL, (S)-HbHNL, (S)-HNL, (S)-Hydroxynitrile lyase, (S)-Me-HNL, (S)-MeHNL, (S)-Oxynitrilase, (S)-selective HNL, (S)-selective hydroxynitrile lyase, Acetone cyanohydrin lyase, ACL, alpha-Hydroxynitrile lyase, EC 4.1.2.37, EC 4.1.2.39, Hb-HNL, HbHNL, HNL, HNL1, Hydroxynitrile lyase, LuHNL, MeHNKL, MeHNL, mut-HNL1, Oxynitrilase, S-HnL, S-hydroxynitrile lyase, S-selective HnL, S-selective hydroxynitrile lyase, S-stereoselective HNL
ECTree
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Turnover Number
Turnover Number on EC 4.1.2.47 - (S)-hydroxynitrile lyase
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9.27
(R)-mandelonitrile
pH 4.23, 25°C, mutant enzyme H103C/N156D
12.7
(R)-mandelonitrile
pH 4.23, 25°C, mutant enzyme H103C/N156G
1.83
-
in 50 mM citrate buffer (pH 5.0), temperature not specified in the publication
21.5
(S)-mandelonitrile
mutant enzyme H103L, in 100 mM citrate buffer pH above 6.0, temperature not specified in the publication
21.67
(S)-mandelonitrile
-
in 50 mM citrate buffer (pH 5.0), temperature not specified in the publication
23.9
(S)-mandelonitrile
mutant enzyme H103M, in 100 mM citrate buffer pH above 6.0, temperature not specified in the publication
34.5
(S)-mandelonitrile
wild type enzyme, in 100 mM citrate buffer pH above 6.0, temperature not specified in the publication
39.7
(S)-mandelonitrile
mutant enzyme K176P/K199P/K224P, in 100 mM citrate buffer pH above 6.0, temperature not specified in the publication
41.8
(S)-mandelonitrile
mutant enzyme K176P, in 100 mM citrate buffer pH above 6.0, temperature not specified in the publication
88.1
(S)-mandelonitrile
pH 4.23, 25°C, mutant enzyme H103C/N156D
0.1
mutant enzyme F125T/L146M, pH and temperature not specified in the publication
0.1
2-nitro-1-phenylethanol
mutant enzyme L121Y/L146M, pH and temperature not specified in the publication
0.1
2-nitro-1-phenylethanol
mutant enzyme V106F, pH and temperature not specified in the publication
0.1
2-nitro-1-phenylethanol
mutant enzyme V106F/L121Y, pH and temperature not specified in the publication
0.12
2-nitro-1-phenylethanol
mutant enzyme F125T/Y133F, pH and temperature not specified in the publication
0.15
2-nitro-1-phenylethanol
wild type enzyme, pH and temperature not specified in the publication
0.2
2-nitro-1-phenylethanol
mutant enzyme C81L, pH and temperature not specified in the publication
0.2
2-nitro-1-phenylethanol
mutant enzyme V106F/F125T, pH and temperature not specified in the publication
0.2
2-nitro-1-phenylethanol
mutant enzyme V106F/L146M, pH and temperature not specified in the publication
0.3
2-nitro-1-phenylethanol
mutant enzyme Y133F, pH and temperature not specified in the publication
0.5
2-nitro-1-phenylethanol
mutant enzyme L121Y, pH and temperature not specified in the publication
0.5
2-nitro-1-phenylethanol
mutant enzyme L121Y/F125T/L146M, pH and temperature not specified in the publication
0.5
2-nitro-1-phenylethanol
mutant enzyme L121Y/F125T/Y133F, pH and temperature not specified in the publication
0.5
2-nitro-1-phenylethanol
mutant enzyme L146M, pH and temperature not specified in the publication
0.5
2-nitro-1-phenylethanol
mutant enzyme V106F/L121Y/F125T, pH and temperature not specified in the publication
0.6
2-nitro-1-phenylethanol
mutant enzyme F125T, pH and temperature not specified in the publication
47.4
benzaldehyde
mutant enzyme H103Y, in 100 mM citrate buffer pH 5.0, temperature not specified in the publication
83.2
benzaldehyde
mutant enzyme K176P, in 100 mM citrate buffer pH 5.0, temperature not specified in the publication
90.4
benzaldehyde
mutant enzyme H103L, in 100 mM citrate buffer pH 5.0, temperature not specified in the publication
93.5
benzaldehyde
mutant enzyme K176P/K199P/K224P, in 100 mM citrate buffer pH 5.0, temperature not specified in the publication
96.1
benzaldehyde
mutant enzyme H103M, in 100 mM citrate buffer pH 5.0, temperature not specified in the publication
96.6
benzaldehyde
wild type enzyme, in 100 mM citrate buffer pH 5.0, temperature not specified in the publication
3
mutant enzyme I209G, pH and temperature not specified in the publication
7
mandelonitrile
mutant enzyme L152F, pH and temperature not specified in the publication
7
mandelonitrile
mutant enzyme V106F, pH and temperature not specified in the publication
17
mandelonitrile
mutant enzyme L148F, pH and temperature not specified in the publication
20
mandelonitrile
mutant enzyme L146M, pH and temperature not specified in the publication
21
mandelonitrile
mutant enzyme F210I, pH and temperature not specified in the publication
22
mandelonitrile
mutant enzyme Y133F, pH and temperature not specified in the publication
23
mandelonitrile
mutant enzyme I209A, pH and temperature not specified in the publication
25
mandelonitrile
wild type enzyme, pH and temperature not specified in the publication
32
mandelonitrile
mutant enzyme F125T/L146M, pH and temperature not specified in the publication
32
mandelonitrile
mutant enzyme F125T/Y133F, pH and temperature not specified in the publication
33
mandelonitrile
mutant enzyme C81L, pH and temperature not specified in the publication
43
mandelonitrile
mutant enzyme L121Y/F125T, pH and temperature not specified in the publication
45
mandelonitrile
mutant enzyme L121Y/F125T/L146M, pH and temperature not specified in the publication
55
mandelonitrile
mutant enzyme V106F/L121Y, pH and temperature not specified in the publication
66
mandelonitrile
mutant enzyme F125T, pH and temperature not specified in the publication
82
mandelonitrile
mutant enzyme V106F/L121Y/F125T, pH and temperature not specified in the publication
106
mandelonitrile
mutant enzyme L121Y, pH and temperature not specified in the publication
0.1
pH 5.0, temperature not specified in the publication, mutant enzyme F125T/L146M
0.1
rac-2-nitro-1-phenylethanol
pH 5.0, temperature not specified in the publication, mutant enzyme V106F
0.12
rac-2-nitro-1-phenylethanol
pH 5.0, temperature not specified in the publication, mutant enzyme F125T/Y133F
0.15
rac-2-nitro-1-phenylethanol
pH 5.0, temperature not specified in the publication, wild-type enzyme
0.2
rac-2-nitro-1-phenylethanol
pH 5.0, temperature not specified in the publication, mutant enzyme V106F/F125T
0.3
rac-2-nitro-1-phenylethanol
pH 5.0, temperature not specified in the publication, mutant enzyme Y133F
0.6
rac-2-nitro-1-phenylethanol
pH 5.0, temperature not specified in the publication, mutant enzyme F125T
3
pH 5.0, temperature not specified in the publication, mutant enzyme I209G
3
rac-mandelonitrile
pH 5.0, temperature not specified in the publication, mutant enzyme L121T
5
rac-mandelonitrile
pH 5.0, temperature not specified in the publication, mutant enzyme L121H
6
rac-mandelonitrile
pH 5.0, temperature not specified in the publication, mutant enzyme L121Q
7
rac-mandelonitrile
pH 5.0, temperature not specified in the publication, mutant enzyme L152F
7
rac-mandelonitrile
pH 5.0, temperature not specified in the publication, mutant enzyme V106F
9
rac-mandelonitrile
pH 5.0, temperature not specified in the publication, mutant enzyme L121S
15
rac-mandelonitrile
pH 5.0, temperature not specified in the publication, mutant enzyme L121V
15
rac-mandelonitrile
pH 5.0, temperature not specified in the publication, mutant enzyme L121W
17
rac-mandelonitrile
pH 5.0, temperature not specified in the publication, mutant enzyme L148F
20
rac-mandelonitrile
pH 5.0, temperature not specified in the publication, mutant enzyme L121C
20
rac-mandelonitrile
pH 5.0, temperature not specified in the publication, mutant enzyme L146M
21
rac-mandelonitrile
pH 5.0, temperature not specified in the publication, mutant enzyme F210I
22
rac-mandelonitrile
pH 5.0, temperature not specified in the publication, mutant enzyme Y133F
23
rac-mandelonitrile
pH 5.0, temperature not specified in the publication, mutant enzyme I209A
23
rac-mandelonitrile
pH 5.0, temperature not specified in the publication, mutant enzyme L121A
25
rac-mandelonitrile
pH 5.0, temperature not specified in the publication, wild-type enzyme
32
rac-mandelonitrile
pH 5.0, temperature not specified in the publication, mutant enzyme F125T/L146M
32
rac-mandelonitrile
pH 5.0, temperature not specified in the publication, mutant enzyme F125T/Y133F
33
rac-mandelonitrile
pH 5.0, temperature not specified in the publication, mutant enzyme C81L
33
rac-mandelonitrile
pH 5.0, temperature not specified in the publication, mutant enzyme L121I
43
rac-mandelonitrile
pH 5.0, temperature not specified in the publication, mutant enzyme L121M
43
rac-mandelonitrile
pH 5.0, temperature not specified in the publication, mutant enzyme L121Y/F125T
45
rac-mandelonitrile
pH 5.0, temperature not specified in the publication, mutant enzyme L121Y/F125T/L146M
55
rac-mandelonitrile
pH 5.0, temperature not specified in the publication, mutant enzyme V106F/L121Y
66
rac-mandelonitrile
pH 5.0, temperature not specified in the publication, mutant enzyme F125T
77
rac-mandelonitrile
pH 5.0, temperature not specified in the publication, mutant enzyme L121F
82
rac-mandelonitrile
pH 5.0, temperature not specified in the publication, mutant enzyme V106F/L121Y/F125T
106
rac-mandelonitrile
pH 5.0, temperature not specified in the publication, mutant enzyme L121Y