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4.1.1.90: peptidyl-glutamate 4-carboxylase

This is an abbreviated version!
For detailed information about peptidyl-glutamate 4-carboxylase, go to the full flat file.

Word Map on EC 4.1.1.90

Reaction

peptidyl-4-carboxyglutamate
+
2,3-Epoxyphylloquinone
 +
H2O
=
peptidyl-glutamate
 +
CO2
 +
O2
 +
phylloquinol

Synonyms

carboxylase, Ci-GGC, gamma glutamyl carboxylase, gamma-carboxylase, gamma-glutamyl carboxylase, GGCX, glutamate carboxylase, matrix gamma-carboxyglutamate protein, matrix Gla protein, peptidyl-glutamate 4-carboxylase, peptidyl-glutamate 4-carboxylase (2-methyl-3-phytyl-1,4-naphthoquinone-epoxidizing), two-chain carboxylase, vitamin K carboxylase, vitamin K-dependent carboxylase, vitamin K-dependent gamma-glutamyl carboxylase, vitamin K-dependent gamma-glutamylcarboxylase, VKC, VKD carboxylase

ECTree

     4 Lyases
         4.1 Carbon-carbon lyases
             4.1.1 Carboxy-lyases
                4.1.1.90 peptidyl-glutamate 4-carboxylase

Purification

Purification on EC 4.1.1.90 - peptidyl-glutamate 4-carboxylase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
affinity chromatography
degree of purification is about 7000fold with reference to ammonium sulfate-fractionated microsomal protein from liver. Purification of carboxylase, solubilized microsomes: 281000 cpm/mg/h, flow-through of Affi-FIXQ/S: 200000 cpm/mg/h, bound to Affi-FIXQ/S: 140000000 cpm/mg/h, affinity-purified carboxylase: 1930000000 cpm/mg/h
difficult, different methods shown
-
inactivated His6-carboxylase-Ac-FLEEL purified under denaturing conditions by Ni-chelation chromatography followed by preparative polyacrylamide gel electrophoresis
partial purification of the enzyme by antibody affinity techniques. Purified 500fold by adsorption to an antiprothrombin column and elution with a dodeca peptide which competes with a prothrombin precursor enzyme recognition site. The purified enzyme is devoid of bound precursors, and has the same ratio of vitamin K epoxidase activity to carboxylase activity as the crude microsomal preparation