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4.1.1.90: peptidyl-glutamate 4-carboxylase

This is an abbreviated version!
For detailed information about peptidyl-glutamate 4-carboxylase, go to the full flat file.

Word Map on EC 4.1.1.90

Reaction

peptidyl-4-carboxyglutamate
+
2,3-Epoxyphylloquinone
 +
H2O
=
peptidyl-glutamate
 +
CO2
 +
O2
 +
phylloquinol

Synonyms

carboxylase, Ci-GGC, gamma glutamyl carboxylase, gamma-carboxylase, gamma-glutamyl carboxylase, GGCX, glutamate carboxylase, matrix gamma-carboxyglutamate protein, matrix Gla protein, peptidyl-glutamate 4-carboxylase, peptidyl-glutamate 4-carboxylase (2-methyl-3-phytyl-1,4-naphthoquinone-epoxidizing), two-chain carboxylase, vitamin K carboxylase, vitamin K-dependent carboxylase, vitamin K-dependent gamma-glutamyl carboxylase, vitamin K-dependent gamma-glutamylcarboxylase, VKC, VKD carboxylase

ECTree

     4 Lyases
         4.1 Carbon-carbon lyases
             4.1.1 Carboxy-lyases
                4.1.1.90 peptidyl-glutamate 4-carboxylase

Molecular Weight

Molecular Weight on EC 4.1.1.90 - peptidyl-glutamate 4-carboxylase

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MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
130000
SDS-PAGE, Sf21 cells transfected with the carboxylase cDNA-containing plasmid, Western Blot
33000
cells transfected with the CAT-V5/His plasmid, Western Blot
60000
proteinase K digestion of the hGC-FLAG reveals a 60 kDa fragment, indicating the lumenal location of the FLAG tag and therefore the carboxyl-terminus of the carboxylase. In contrast, FLAG-hGC does not show a proteinase K–resistant fragment except for the residual undigested full-length carboxylase, which indicates the cytoplasmic location of the FLAG tag and therefore the amino-terminus of the carboxylase
77000
single major band on SDS gel electrophoresis. The eluted protein contains both stable vitamin K-dependent carboxylase and vitamin K epoxidase activity
85700
calculated from sequence
87540
calculated from sequence
94000
95000
full-length carboxylase
99000
SDS-PAGE, Western Blot. CHO cells transfected with the cDNA for wild type FLAG-vitamin K-dependent gamma-glutamyl carboxylase
additional information
-
determination of disulfide bond formation in purified two-chain carboxylase and P80L and P378L two-chain carboxylases by SDS-PAGE and Western Blot analyses