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3.8.1.2: (S)-2-haloacid dehalogenase

This is an abbreviated version!
For detailed information about (S)-2-haloacid dehalogenase, go to the full flat file.

Word Map on EC 3.8.1.2

Reaction

(S)-2-haloacid
+
H2O
=
(R)-2-hydroxyacid
 +
halide

Synonyms

2-HAD, 2-halo acid dehalogenase, 2-haloacid dehalogenase, 2-haloacid dehalogenase[ambiguous], 2-haloacid halidohydrolase[ambiguous], 2-haloalkanoate dehalogenase, 2-haloalkanoic acid dehalogenase, 2-haloalkanoid acid halidohydrolase, 2-halocarboxylic acid dehalogenase II, DEH99, dehalogenase IVa, DehL, DL-2-haloacid dehalogenase [ambiguous], HADIIBSW, HadL AJ1, L-2-DhlB, L-2-haloacid dehalogenase, L-2-MCPA dehalogenase, L-2_HAD, L-DEX, L-DEX YL, L-DEXs, L-HAD, L-HADST, L-haloacid dehalogenase, PH1421, Rhodobacteraceae family L-haloacid dehalogenase, S-2-haloacid dehalogenase, STK_25700

ECTree

     3 Hydrolases
         3.8 Acting on halide bonds
             3.8.1 In carbon-halide compounds
                3.8.1.2 (S)-2-haloacid dehalogenase

Temperature Stability

Temperature Stability on EC 3.8.1.2 - (S)-2-haloacid dehalogenase

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TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
30 - 70
recombinant enzyme DehRhb has a melting temperature of 67°C, it is completely stable at 30°C, loses 10% activity at 40° and 30% at 55°C after 90mmin, 85% activity are remaining after 90 min at 50°C, 14% activity remaining after 90 min at 60°C
40
-
purified recombinant enzyme, 2 h, completely stable up to
50
-
30 min, 50% loss of activity
50 - 90
the enzyme is stable to prolonged exposure to 50°C, losing only 8% activity after 90 min incubation, as the temperature is increased the stability decreases, the enzyme is thermostable with a half-life of over 1 h at 70°C
55
-
after 15 min loss of 50% activity
65
-
purified recombinant enzyme, 2 h, inactivation
70
4 h, enzyme remains fully active
80
4 h, 10% loss of activity
90
1 h, 70% loss of activity
additional information
-
activity and stability at different temperatures