3.8.1.2: (S)-2-haloacid dehalogenase
This is an abbreviated version!
For detailed information about (S)-2-haloacid dehalogenase, go to the full flat file.
Word Map on EC 3.8.1.2
-
3.8.1.2
-
dehalogenation
-
synthesis
-
haloacids
-
l-2-haloacids
-
bioremediation
-
l-2-chloropropionate
-
2-chloropropionic
-
halide
-
hymeniacidon
-
monochloroacetic
-
xanthobacter
-
dechlorination
-
autotrophicus
-
monobromoacetic
-
chloroacetate
-
haloalkane
-
analysis
-
environmental protection
- 3.8.1.2
-
dehalogenation
- synthesis
-
haloacids
- l-2-haloacids
-
bioremediation
- l-2-chloropropionate
-
2-chloropropionic
- halide
- hymeniacidon
-
monochloroacetic
-
xanthobacter
-
dechlorination
- autotrophicus
-
monobromoacetic
- chloroacetate
- haloalkane
- analysis
- environmental protection
Reaction
Synonyms
2-HAD, 2-halo acid dehalogenase, 2-haloacid dehalogenase, 2-haloacid dehalogenase[ambiguous], 2-haloacid halidohydrolase[ambiguous], 2-haloalkanoate dehalogenase, 2-haloalkanoic acid dehalogenase, 2-haloalkanoid acid halidohydrolase, 2-halocarboxylic acid dehalogenase II, DEH99, dehalogenase IVa, DehL, DL-2-haloacid dehalogenase [ambiguous], HADIIBSW, HadL AJ1, L-2-DhlB, L-2-haloacid dehalogenase, L-2-MCPA dehalogenase, L-2_HAD, L-DEX, L-DEX YL, L-DEXs, L-HAD, L-HADST, L-haloacid dehalogenase, PH1421, Rhodobacteraceae family L-haloacid dehalogenase, S-2-haloacid dehalogenase, STK_25700
ECTree
3.8.1.2 (S)-2-haloacid dehalogenaseAdvanced search results
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results (Substrates Products
Substrates Products on EC 3.8.1.2 - (S)-2-haloacid dehalogenase
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SUBSTRATE 
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
(R,S)-2-bromopropionic acid + H2O
(S)-lactic acid + (S)-2-bromopropionic acid + HCl
-
-
-
-
?
(S)-2-bromopropionic acid + H2O
(R)-2-hydroxypropionate + bromide
-
-
-
?
(S)-2-chloropropionic acid + H2O
(R)-2-hydroxypropionate + chloride
-
-
-
?
2-bromoacetic acid + H2O
2-hydroxyacetate + bromide
-
-
-
?
2-chloroacetic acid + H2O
2-hydroxyacetate + chloride
-
-
-
?
2-chlorobutyrate + H2O
2-hydroxybutyrate + chloride
Stutzerimonas chloritidismutans
-
low activity
-
-
?
bromoacetate + H2O
hydroxyacetate + bromide
Stutzerimonas chloritidismutans
-
highest activity
-
-
?
chloroacetate + H2O
hydroxyacetate + chloride
Stutzerimonas chloritidismutans
-
high activity
-
-
?
DL-2-chloropropionate + H2O
DL-2-hydroxypropionate + chloride
Stutzerimonas chloritidismutans
-
high activity
-
-
?
L-2-bromopropionate + H2O
D-2-hydroxypropionate + bromide
Stutzerimonas chloritidismutans
-
high activity
-
-
?
L-2-chloropropionic acid + H2O
D-2-hydroxypropionate + HCl
-
-
-
-
?
(R,S)-2-chloropropionic acid + H2O
(S)-lactic acid + (S)-2-chloropropionic acid + HCl
-
-
-
-
?
(R,S)-2-chloropropionic acid + H2O
(S)-lactic acid + (S)-2-chloropropionic acid + HCl
-
-
-
-
?
(R,S)-2-chloropropionic acid + H2O
(S)-lactic acid + (S)-2-chloropropionic acid + HCl
-
-
-
-
?
(S)-2-bromopropionic acid + H2O
(R)-2-hydroxypropionic acid + bromide
-
-
-
-
?
(S)-2-bromopropionic acid + H2O
(R)-2-hydroxypropionic acid + bromide
-
41% activity compared to monobromoacetic acid
-
-
?
(S)-2-bromopropionic acid + H2O
(R)-2-hydroxypropionic acid + bromide
-
41% activity compared to monobromoacetic acid
-
-
?
(S)-2-chlorobutyric acid + H2O
(R)-2-hydroxybutyric acid + chloride
-
below 10% activity compared to monobromoacetic acid
-
-
?
(S)-2-chlorobutyric acid + H2O
(R)-2-hydroxybutyric acid + chloride
-
below 10% activity compared to monobromoacetic acid
-
-
?
(S)-2-chloropropionate + H2O
(R)-2-hydroxypropionate + chloride
-
-
-
?
(S)-2-chloropropionate + H2O
(R)-2-hydroxypropionate + chloride
the wild-type enzyme shows no activity with the (R)-enantiomer
-
-
?
(S)-2-chloropropionate + H2O
(R)-2-hydroxypropionic acid + chloride
-
-
-
?
(S)-2-chloropropionate + H2O
(R)-2-hydroxypropionic acid + chloride
the enzyme acts exclusively on (S)-isomers
-
-
?
(S)-2-chloropropionic acid + H2O
(R)-2-hydroxypropionic acid + chloride
-
-
-
-
?
(S)-2-chloropropionic acid + H2O
(R)-2-hydroxypropionic acid + chloride
-
high activity
-
-
?
(S)-2-chloropropionic acid + H2O
(R)-2-hydroxypropionic acid + chloride
-
enantioselective
-
-
?
(S)-2-chloropropionic acid + H2O
(R)-2-hydroxypropionic acid + chloride
-
14% activity compared to monobromoacetic acid
-
-
?
(S)-2-chloropropionic acid + H2O
(R)-2-hydroxypropionic acid + chloride
-
14% activity compared to monobromoacetic acid
-
-
?
(S)-2-chloropropionic acid + H2O
(R)-2-hydroxypropionic acid + chloride
-
-
-
-
?
(S)-2-chloropropionic acid + H2O
(R)-2-hydroxypropionic acid + chloride
-
the substrate interacts strongly with Asp10, Arg41, Lys151, and Asp180
-
-
?
(S)-2-chloropropionic acid + H2O
(R)-2-hydroxypropionic acid + chloride
-
-
-
-
?
(S)-2-haloacid + H2O
(R)-2-hydroxyacid + halide
-
-
-
-
?
(S)-2-haloacid + H2O
(R)-2-hydroxyacid + halide
-
reaction by inversion of the configuration in a mixture of dimethylsulfoxide and buffer, but in presence of stoichiometric amounts of water proceeds without inversion of the configuration contrary to the behavior in an aqueous environment
-
-
?
(S)-2-haloacid + H2O
(R)-2-hydroxyacid + halide
-
reaction by inversion of the configuration in a mixture of dimethylsulfoxide and buffer, but in presence of stoichiometric amounts of water proceeds without inversion of the configuration contrary to the behavior in an aqueous environment
-
-
?
(S)-2-haloacid + H2O
(R)-2-hydroxyacid + halide
-
-
-
-
?
(S)-2-haloacid + H2O
(R)-2-hydroxyacid + halide
-
enzyme of 2-chloropropionate grown cells
-
-
?
(S)-2-haloacid + H2O
(R)-2-hydroxyacid + halide
-
substrate specificity towards chlorinated and bromated substrates is limited to short chain monosubstituated 2-halocarboxylic acids, fluoro compounds are not converted
-
-
?
(S)-2-haloacid + H2O
(R)-2-hydroxyacid + halide
-
substrate specificity towards chlorinated and bromated substrates is limited to short chain monosubstituated 2-halocarboxylic acids, fluoro compounds are not converted
-
-
?
(S)-2-haloacid + H2O
(R)-2-hydroxyacid + halide
-
-
-
-
?
(S)-2-haloacid + H2O
(R)-2-hydroxyacid + halide
-
-
-
?
(S)-2-haloacid + H2O
(R)-2-hydroxyacid + halide
-
-
-
-
?
(S)-2-haloacid + H2O
(R)-2-hydroxyacid + halide
-
-
-
-
?
2,2-dichloropropionate + H2O
propionate + HCl
-
53% of the activity compared to monochloroacetate
-
-
?
2,2-dichloropropionate + H2O
propionate + HCl
-
53% of the activity compared to monochloroacetate
-
-
?
2,3-dichloropropionic acid + H2O
3-chlorlactic acid + HCl
-
-
-
?
2-chloropropionate + H2O
2-hydroxypropionate + chloride
-
-
-
-
?
D-2-bromopropionate + H2O
L-2-hydroxypropionate + bromide
Stutzerimonas chloritidismutans
-
high activity
-
-
?
D-2-bromopropionate + H2O
L-2-hydroxypropionate + bromide
Stutzerimonas chloritidismutans AW-1T
-
high activity
-
-
?
D-2-chloropropionate + H2O
L-2-hydroxypropionate + chloride
Stutzerimonas chloritidismutans
-
very low activity
-
-
?
D-2-chloropropionate + H2O
L-2-hydroxypropionate + chloride
Stutzerimonas chloritidismutans AW-1T
-
very low activity
-
-
?
dichloroacetic acid + H2O
?
-
below 10% activity compared to monobromoacetic acid
-
-
?
dichloroacetic acid + H2O
?
-
below 10% activity compared to monobromoacetic acid
-
-
?
DL-2-haloalkanoic acid + H2O
L-2-hydroxyalkanoic acid + D-2-hydroxyalkanoic acid + halide
-
-
-
-
?
DL-2-haloalkanoic acid + H2O
L-2-hydroxyalkanoic acid + D-2-hydroxyalkanoic acid + halide
-
-
-
-
?
L-2-bromobutanoate + H2O
2-hydroxybutanoate + HBr
the enzyme catalyzes the stereospecific removal of bromide from the L-conformer but not the D-conformer
-
-
?
L-2-bromobutanoate + H2O
2-hydroxybutanoate + HBr
the enzyme catalyzes the stereospecific removal of bromide from the L-conformer but not the D-conformer
-
-
?
L-2-bromohexanoate + H2O
2-hydroxyhexanoate + HBr
the enzyme catalyzes the stereospecific removal of bromide from the L-conformer but not the D-conformer
-
-
?
L-2-bromohexanoate + H2O
2-hydroxyhexanoate + HBr
the enzyme catalyzes the stereospecific removal of bromide from the L-conformer but not the D-conformer
-
-
?
L-2-bromopropionate + H2O
lactate + HBr
the enzyme catalyzes the stereospecific removal of bromide from the L-conformer but not the D-conformer
-
-
?
L-2-bromopropionate + H2O
lactate + HBr
the enzyme catalyzes the stereospecific removal of bromide from the L-conformer but not the D-conformer
-
-
?
L-2-chloropropionate + H2O
D-2-hydroxypropionate + chloride
Stutzerimonas chloritidismutans
-
high activity
-
-
?
L-2-chloropropionate + H2O
D-2-hydroxypropionate + chloride
Stutzerimonas chloritidismutans AW-1T
-
high activity
-
-
?
L-2-chloropropionate + H2O
lactate + HCl
the enzyme catalyzes the stereospecific removal of chloride from the L-conformer but not the D-conformer
-
-
?
L-2-chloropropionate + H2O
lactate + HCl
the enzyme catalyzes the stereospecific removal of chloride from the L-conformer but not the D-conformer
-
-
?
L-2-chloropropionic acid + H2O
D-2-hydroxypropionic acid + HCl
-
-
-
-
?
L-2-chloropropionic acid + H2O
D-2-hydroxypropionic acid + HCl
-
-
-
-
?
L-2-haloalkanoic acid + H2O
D-2-hydroxyalkanoic acid + halide
-
-
-
-
?
L-2-haloalkanoic acid + H2O
D-2-hydroxyalkanoic acid + halide
-
-
-
-
?
L-2-haloalkanoic acid + H2O
D-2-hydroxyalkanoic acid + halide
-
-
-
?
L-2-haloalkanoic acid + H2O
D-2-hydroxyalkanoic acid + halide
-
-
-
-
?
L-2-haloalkanoic acid + H2O
D-2-hydroxyalkanoic acid + halide
-
-
-
-
?
L-2-haloalkanoic acid + H2O
D-2-hydroxyalkanoic acid + halide
-
-
-
?
L-2-haloalkanoic acid + H2O
D-2-hydroxyalkanoic acid + halide
-
-
-
-
?
L-2-haloalkanoic acid + H2O
D-2-hydroxyalkanoic acid + halide
-
-
-
-
?
L-2-haloalkanoic acid + H2O
D-2-hydroxyalkanoic acid + halide
-
-
-
-
?
L-2-haloalkanoic acid + H2O
D-2-hydroxyalkanoic acid + halide
-
-
-
-
?
L-2-haloalkanoic acid + H2O
D-2-hydroxyalkanoic acid + halide
-
-
-
-
?
L-2-haloalkanoic acid + H2O
D-2-hydroxyalkanoic acid + halide
-
-
-
-
?
L-2-haloalkanoic acid + H2O
D-2-hydroxyalkanoic acid + halide
-
-
-
-
?
L-2-haloalkanoic acid + H2O
D-2-hydroxyalkanoic acid + halide
-
-
-
?
L-2-haloalkanoic acid + H2O
D-2-hydroxyalkanoic acid + halide
-
-
-
-
?
L-2-monochloropropionate + H2O
D-2-hydroxypropionate + HCl
-
activity with D-2-monochloropropionate is 5% compared to activity with L-2-monochloropropionate
-
-
?
L-2-monochloropropionate + H2O
D-2-hydroxypropionate + HCl
-
activity with D-2-monochloropropionate is 5% compared to activity with L-2-monochloropropionate
-
-
?
monobromoacetate + H2O
glycolate + HBr
-
86% of the activity compared to monochloroacetate
-
-
?
monobromoacetate + H2O
glycolate + HBr
-
86% of the activity compared to monochloroacetate
-
-
?
monobromoacetic acid + H2O
acetic acid + bromide
-
best substrate
-
-
?
monobromoacetic acid + H2O
acetic acid + bromide
-
best substrate
-
-
?
monochloroacetate + H2O
glycolate + HCl
-
-
-
-
?
monoiodoacetate + H2O
glycolate + HI
-
73% of the activity compared to monochloroacetate
-
-
?
monoiodoacetate + H2O
glycolate + HI
-
73% of the activity compared to monochloroacetate
-
-
?
additional information
?
-
-
no activity with 2-fluoropropionic acid
-
-
?
additional information
?
-
the alpha-helical structure of the enzyme plays a significant role during catalysis, while beta-sheet formation is a functional disadvantage. Analysis of environment-induced conformational and functional changes of 2-haloacid dehalogenase is informative for understanding the molecular basis of its stability and the design of the enzyme for future biotechnological applications in conjunction with its biochemical properties
-
-
-
additional information
?
-
-
the alpha-helical structure of the enzyme plays a significant role during catalysis, while beta-sheet formation is a functional disadvantage. Analysis of environment-induced conformational and functional changes of 2-haloacid dehalogenase is informative for understanding the molecular basis of its stability and the design of the enzyme for future biotechnological applications in conjunction with its biochemical properties
-
-
-
additional information
?
-
-
the enzyme reaction mechanism proceeds via an ester intermediate and a nucleophilic aspartate attacking the C-atom of the substrate to displace the halo-atom, mass spectrometral analysis of the structural changes during catalysis, overview
-
-
?
additional information
?
-
-
the enzyme reaction mechanism proceeds via an ester intermediate and a nucleophilic aspartate attacking the C-atom of the substrate to displace the halo-atom, mass spectrometral analysis of the structural changes during catalysis, overview
-
-
?
additional information
?
-
-
L-2-haloacid dehalogenase acts specifically on L-2-haloalkanoic acids to produce D-2-hydroxyalkanoic acids, which have potential applications in chemical industries and bioremediation. No activity with DL-2-fluopropionate, 3-chloropropionate, 2,2-dichloropropionate, DL-2-chloropropionamide, and DL-2-bromopropionamide
-
-
?
additional information
?
-
-
L-2-haloacid dehalogenase acts specifically on L-2-haloalkanoic acids to produce D-2-hydroxyalkanoic acids, which have potential applications in chemical industries and bioremediation. No activity with DL-2-fluopropionate, 3-chloropropionate, 2,2-dichloropropionate, DL-2-chloropropionamide, and DL-2-bromopropionamide
-
-
?
additional information
?
-
-
PH1421 is a magnesium-dependent haloacid dehalogenase phosphatase that acts on compounds closely related to 2-phosphoglycolate, the enzyme does possess 4-nitrophenyl phosphate hydrolase activity in the presence of Mg2+
-
-
?
additional information
?
-
-
PH1421 is a magnesium-dependent haloacid dehalogenase phosphatase that acts on compounds closely related to 2-phosphoglycolate, the enzyme does possess 4-nitrophenyl phosphate hydrolase activity in the presence of Mg2+
-
-
?
additional information
?
-
no activity with 2-chlorobutyric acid, trichloroacetic acid, 3-bromopropionic acid, fluoroacetate, (R)-2-chloropropionic acid, (R)-2-bromoopropionic acid, 1,2-dichloropropane, and 1-bromoheptane
-
-
?
additional information
?
-
Stutzerimonas chloritidismutans
-
no activity with 4-chlorobutyrate, 3-chloropropionate, 3-bromopropionate, 3-iodopropionate, or 2,3-dichloropropionate
-
-
-
additional information
?
-
Stutzerimonas chloritidismutans AW-1T
-
no activity with 4-chlorobutyrate, 3-chloropropionate, 3-bromopropionate, 3-iodopropionate, or 2,3-dichloropropionate
-
-
-
additional information
?
-
the enzyme is not active toward 1,4-dibromobutane
-
-
?
additional information
?
-
the enzyme is not active toward 1,4-dibromobutane
-
-
?
