3.8.1.2: (S)-2-haloacid dehalogenase
This is an abbreviated version!
For detailed information about (S)-2-haloacid dehalogenase, go to the full flat file.
Word Map on EC 3.8.1.2
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3.8.1.2
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dehalogenation
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synthesis
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haloacids
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l-2-haloacids
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bioremediation
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l-2-chloropropionate
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2-chloropropionic
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halide
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hymeniacidon
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monochloroacetic
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xanthobacter
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dechlorination
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autotrophicus
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monobromoacetic
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chloroacetate
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haloalkane
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analysis
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environmental protection
- 3.8.1.2
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dehalogenation
- synthesis
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haloacids
- l-2-haloacids
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bioremediation
- l-2-chloropropionate
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2-chloropropionic
- halide
- hymeniacidon
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monochloroacetic
-
xanthobacter
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dechlorination
- autotrophicus
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monobromoacetic
- chloroacetate
- haloalkane
- analysis
- environmental protection
Reaction
Synonyms
2-HAD, 2-halo acid dehalogenase, 2-haloacid dehalogenase, 2-haloacid dehalogenase[ambiguous], 2-haloacid halidohydrolase[ambiguous], 2-haloalkanoate dehalogenase, 2-haloalkanoic acid dehalogenase, 2-haloalkanoid acid halidohydrolase, 2-halocarboxylic acid dehalogenase II, DEH99, dehalogenase IVa, DehL, DL-2-haloacid dehalogenase [ambiguous], HADIIBSW, HadL AJ1, L-2-DhlB, L-2-haloacid dehalogenase, L-2-MCPA dehalogenase, L-2_HAD, L-DEX, L-DEX YL, L-DEXs, L-HAD, L-HADST, L-haloacid dehalogenase, PH1421, Rhodobacteraceae family L-haloacid dehalogenase, S-2-haloacid dehalogenase, STK_25700
ECTree
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Organic Solvent Stability
Organic Solvent Stability on EC 3.8.1.2 - (S)-2-haloacid dehalogenase
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Acetone
acetonitrile
chloroform
dithiothreitol
DMSO
Ethanol
Methanol
n-heptane
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retaining 3.3% activity of that in water with substrate 2-chloropropionate
additional information
enzyme DehRhb has a relatively high tolerance to organic solvents, i.e. in ethanol, methanol, acetonitrile, or dimethylsulfoxide
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20-40% of activity remains after incubation in 40% of DMSO
acetonitrile
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20-40% of activity remains after incubation in 40% of DMSO
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acetonitrile
in acetonitrile the activity is reduced by over 70% for any concentration tested
acetonitrile
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in acetonitrile the activity is reduced by over 70% for any concentration tested
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DMSO
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about 92% of activity remains after incubation in 40% of DMSO
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DMSO
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the stereochemistry of the product obtained from racemic 2-bromopropionic acid by the action of celite-immobilized disrupted cells in dimethylsulfoxide differs from that of the enantiomer obtained in an aqueous buffer
DMSO
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the stereochemistry of the product obtained from racemic 2-bromopropionic acid by the action of celite-immobilized disrupted cells in dimethylsulfoxide differs from that of the enantiomer obtained in an aqueous buffer
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DMSO
the enzyme is relatively stable to solvents with 25% activity lost when incubated for 1 h in 20% (v/v) DMSO, at higher concentrations of DMSO the activity is reduced to below 40% of normal
DMSO
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the enzyme is relatively stable to solvents with 25% activity lost when incubated for 1 h in 20% (v/v) DMSO, at higher concentrations of DMSO the activity is reduced to below 40% of normal
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Ethanol
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20-40% of activity remains after incubation in 40% of DMSO
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Ethanol
in 10% (v/v) ethanol activity is still 40% of normal, further increases in ethanol concentration reduce the activity until at 50% (v/v) ethanol less than 10% activity is observed
Ethanol
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in 10% (v/v) ethanol activity is still 40% of normal, further increases in ethanol concentration reduce the activity until at 50% (v/v) ethanol less than 10% activity is observed
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Methanol
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20-40% of activity remains after incubation in 40% of DMSO
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Methanol
the activity in 10% (v/v) methanol is only 25% of normal, similar activity is observed for the higher concentrations of methanol
Methanol
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the activity in 10% (v/v) methanol is only 25% of normal, similar activity is observed for the higher concentrations of methanol
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