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3.8.1.2: (S)-2-haloacid dehalogenase

This is an abbreviated version!
For detailed information about (S)-2-haloacid dehalogenase, go to the full flat file.

Word Map on EC 3.8.1.2

Reaction

(S)-2-haloacid
+
H2O
=
(R)-2-hydroxyacid
+
halide

Synonyms

2-HAD, 2-halo acid dehalogenase, 2-haloacid dehalogenase, 2-haloacid dehalogenase[ambiguous], 2-haloacid halidohydrolase[ambiguous], 2-haloalkanoate dehalogenase, 2-haloalkanoic acid dehalogenase, 2-haloalkanoid acid halidohydrolase, 2-halocarboxylic acid dehalogenase II, DEH99, dehalogenase IVa, DehL, DL-2-haloacid dehalogenase [ambiguous], HADIIBSW, HadL AJ1, L-2-DhlB, L-2-haloacid dehalogenase, L-2-MCPA dehalogenase, L-2_HAD, L-DEX, L-DEX YL, L-DEXs, L-HAD, L-HADST, L-haloacid dehalogenase, PH1421, Rhodobacteraceae family L-haloacid dehalogenase, S-2-haloacid dehalogenase, STK_25700

ECTree

     3 Hydrolases
         3.8 Acting on halide bonds
             3.8.1 In carbon-halide compounds
                3.8.1.2 (S)-2-haloacid dehalogenase

Purification

Purification on EC 3.8.1.2 - (S)-2-haloacid dehalogenase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
-
HiPrep 26/10 column chromatography, Super Q Toyopearl 650M column chromatography, hydroxyapatite column chromatography, and Superdex 200 gel filtration
-
Ni-NTA column chromatography and gel filtration
recombinant N-terminally His- and S-tagged enzyme from Escherichia coli strain BMRosetta by affinity purification followed by gel filtration, to homogeneity
-
recombinant N-terminally His-tagged enzyme from Escherichia coli strain BL21(DE3)-RIPL by nickel affinity chromatography and gel filtration
two-step method: heat treatment step that removes 90% of the impurities, followed by an acidification step that eliminates the remaining impurities. The easy purification procedure renders the production of this protein fast, cost effective, and suitable for large-scale production