3.8.1.2: (S)-2-haloacid dehalogenase
This is an abbreviated version!
For detailed information about (S)-2-haloacid dehalogenase, go to the full flat file.
Word Map on EC 3.8.1.2
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3.8.1.2
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dehalogenation
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synthesis
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haloacids
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l-2-haloacids
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bioremediation
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l-2-chloropropionate
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2-chloropropionic
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halide
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hymeniacidon
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monochloroacetic
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xanthobacter
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dechlorination
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autotrophicus
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monobromoacetic
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chloroacetate
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haloalkane
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analysis
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environmental protection
- 3.8.1.2
-
dehalogenation
- synthesis
-
haloacids
- l-2-haloacids
-
bioremediation
- l-2-chloropropionate
-
2-chloropropionic
- halide
- hymeniacidon
-
monochloroacetic
-
xanthobacter
-
dechlorination
- autotrophicus
-
monobromoacetic
- chloroacetate
- haloalkane
- analysis
- environmental protection
Reaction
Synonyms
2-HAD, 2-halo acid dehalogenase, 2-haloacid dehalogenase, 2-haloacid dehalogenase[ambiguous], 2-haloacid halidohydrolase[ambiguous], 2-haloalkanoate dehalogenase, 2-haloalkanoic acid dehalogenase, 2-haloalkanoid acid halidohydrolase, 2-halocarboxylic acid dehalogenase II, DEH99, dehalogenase IVa, DehL, DL-2-haloacid dehalogenase [ambiguous], HADIIBSW, HadL AJ1, L-2-DhlB, L-2-haloacid dehalogenase, L-2-MCPA dehalogenase, L-2_HAD, L-DEX, L-DEX YL, L-DEXs, L-HAD, L-HADST, L-haloacid dehalogenase, PH1421, Rhodobacteraceae family L-haloacid dehalogenase, S-2-haloacid dehalogenase, STK_25700
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Purification
Purification on EC 3.8.1.2 - (S)-2-haloacid dehalogenase
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HiPrep 26/10 column chromatography, Super Q Toyopearl 650M column chromatography, hydroxyapatite column chromatography, and Superdex 200 gel filtration
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recombinant N-terminally His- and S-tagged enzyme from Escherichia coli strain BMRosetta by affinity purification followed by gel filtration, to homogeneity
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recombinant N-terminally His-tagged enzyme from Escherichia coli strain BL21(DE3)-RIPL by nickel affinity chromatography and gel filtration
two-step method: heat treatment step that removes 90% of the impurities, followed by an acidification step that eliminates the remaining impurities. The easy purification procedure renders the production of this protein fast, cost effective, and suitable for large-scale production