Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.8.1.2 extracted from

  • Diez, A.; Prieto, M.I.; Alvarez, M.J.; Bautista, J.M.; Garrido, A.; Puyet, A.
    Improved catalytic performance of a 2-haloacid dehalogenase from Azotobacter sp. by ion-exchange immobilisation (1996), Biochem. Biophys. Res. Commun., 220, 828-833.
    View publication on PubMed
Search for more literature for 3.8.1.2

Application

Application Comment Organism
environmental protection detoxification of halogenated herbicides, solvents and other xenobiotic compounds by immobilized enzyme Azotobacter sp.
synthesis production of optically active 2-hydroxyalkanoic acids and 2-haloalkanoic acids for chiral synthesis in industry Azotobacter sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.1
-
 Monochloroacetate pH 8.0, 30°C Azotobacter sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-2-haloalkanoic acid + H2O Azotobacter sp.
-
 D-2-hydroxyalkanoic acid + halide
-
 ?
L-2-haloalkanoic acid + H2O Azotobacter sp. RC26
-
 D-2-hydroxyalkanoic acid + halide
-
 ?

Organism

Organism UniProt Comment Textmining
Azotobacter sp.
-
-
-
Azotobacter sp. RC26
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Azotobacter sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-2-haloacid + H2O
-
 Azotobacter sp. (R)-2-hydroxyacid + halide
-
 ?
(S)-2-haloacid + H2O
-
 Azotobacter sp. RC26 (R)-2-hydroxyacid + halide
-
 ?
L-2-haloalkanoic acid + H2O
-
 Azotobacter sp. D-2-hydroxyalkanoic acid + halide
-
 ?
L-2-haloalkanoic acid + H2O
-
 Azotobacter sp. RC26 D-2-hydroxyalkanoic acid + halide
-
 ?
monochloroacetate + H2O
-
 Azotobacter sp. glycolate + HCl
-
 ?
monochloroacetate + H2O
-
 Azotobacter sp. RC26 glycolate + HCl
-
 ?

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
20 60
-
 Azotobacter sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
additional information
-
 activity and stability at different temperatures Azotobacter sp.

pH Range

pH Minimum pH Maximum Comment Organism
8 10
-
 Azotobacter sp.