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3.6.5.6: tubulin GTPase

This is an abbreviated version!
For detailed information about tubulin GTPase, go to the full flat file.

Word Map on EC 3.6.5.6

Reaction

GTP
+
H2O
=
GDP
 +
phosphate

Synonyms

atypical GTPase, BtubB, cell division protein, EC 3.6.1.51, eFtsZ, FtsZ, FtsZ tubulin-like protein, FtsZDr, gamma-tubulin, GTP phosphohydrolase, GTPase, guanine triphosphatase, guanosine 5'-triphosphatase, guanosine triphosphatase, mFtsZ, ORF156, ribosomal GTPase, TUBG1, tubulin, tubulin FtsZ, tubulin GTPase, tubulin homolog FtsZ, tubulin-colchicine GTPase

ECTree

     3 Hydrolases
         3.6 Acting on acid anhydrides
             3.6.5 Acting on GTP to facilitate cellular and subcellular movement
                3.6.5.6 tubulin GTPase

Purification

Purification on EC 3.6.5.6 - tubulin GTPase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
bovine brain tubulin is purified by two cycles of polymerization and depolymerization
-
goat brain microtubule protein is isolated by two cycles of polymerization and depolymerization, MAP-free tubulin is purified from the microtubule protein by phosphocellulose chromatography
-
MAP-free tubulin is purified from the microtubule protein of goat brain by phosphocellulose chromatography
-
microtubules are prepared from freshly isolated lens epithelial fractions from mice
-
ovine brain tubulin is prepared from freshly-killed animals using phosphocellulose chromatography
-
purified to ca. 35%
-
recombinant His-tagged beta-tubulin mutant T238A from Saccharomyces cerevisiae by nickel affinity chromatography
-
recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3) by dialysis and nickel affinity chromatography, tag cleavage by thrombin, and dialysis
using a glutathione-Sepharose affinity column
using a Ni-nitrilotriacetic acid resin column
-