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Literature summary for 3.6.5.6 extracted from
- Alpha-crystallin expression affects microtubule assembly and prevents their aggregation (2006), FASEB J., 20, 846-857.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| tubulin | unassembled tubulin denatures relatively easily, and the denatured tubulin can inhibit the assembly itself | Mus musculus |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 49800 | - |
theoretical molecular mass, beta tubulin | Mus musculus |
| 49800 | - |
theoretical molecular mass, beta tubulin | Bos taurus |
| 50000 | - |
alpha-beta tubulin | Bos taurus |
| 50000 | - |
determined by SDS-PAGE and immunoblot analysis | Mus musculus |
| 50130 | - |
theoretical molecular mass, alpha tubulin | Mus musculus |
| 50130 | - |
theoretical molecular mass, alpha tubulin | Bos taurus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| GTP + H2O | Mus musculus | - |
GDP + phosphate | - |
ir |  |
| GTP + H2O | Bos taurus | - |
GDP + phosphate | - |
ir | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bos taurus | - |
- |
- |
| Mus musculus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| microtubules are prepared from freshly isolated lens epithelial fractions from mice | Mus musculus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| brain | - |
Bos taurus | - |
| lens | lens epithelial cell | Mus musculus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| GTP + H2O | - |
Mus musculus | GDP + phosphate | - |
ir | |
| GTP + H2O | - |
Bos taurus | GDP + phosphate | - |
ir | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| heterodimer | - |
Mus musculus |
| heterodimer | - |
Bos taurus |
| More | elongated microtubule protofilaments form at 37°C in the presence of GTP and later close into hollow cylindrical polymers | Mus musculus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| tubulin GTPase | - |
Mus musculus |
| tubulin GTPase | - |
Bos taurus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
elongated microtubule protofilaments form at 37°C in the presence of GTP and later close into hollow cylindrical polymers | Mus musculus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0077 | - |
GTP | in the presence of alphaA-crystallin | Bos taurus | |
| 0.008 | - |
GTP | in the presence of alphaB-crystallin | Bos taurus | |
| 0.0083 | - |
GTP | - |
Bos taurus | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.8 | - |
assembly of microtubules | Mus musculus |
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