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3.6.5.2: small monomeric GTPase

This is an abbreviated version!
For detailed information about small monomeric GTPase, go to the full flat file.

Word Map on EC 3.6.5.2

Reaction

GTP
+
H2O
=
GDP
 +
phosphate

Synonyms

ADP-ribosylation factor 6, ADP-ribosylation factor-like protein 2, Alp41, Alp41/Arl2, ARD1, Arf 1, Arf 1-6, Arf-like 3 small GTPase, Arf6, ARL2, Arl6, Arl6/BBS3, Arl9, AtRopGEF12, Cdc42, Cdc42Hs, Der, Der GTPase, double Era-like protein, EC 3.6.1.47, EF-Tu, EhRab11B, elF-2, EmRas, FgCdc42, FgRac1, FgRHO1, FgRHO2, FgRHO3, FgRHO4, GCC185, Gem, GhRac1 GTPase, Gsp1p, GTP-phosphohydrolase, GTPase, GTPase Alp41/Arl2, GTPase TcRho1, GTPase Toc33, GTPase Toc33/34, guanine triphosphatase, guanosine 5'-triphosphatase, guanosine triphosphatase, H-Ras, K-Ras, Kir, Krev-1, M-Ras, monomeric G protein, monomeric GTPase, More, non-canonical Ras-like G protein, OsRac1, p21 ras, Pc-Rac1, PfG, PRA, Pra2, Pra3, Rab GTPase, Rab GTPase protein 5, Rab11, Rab13, Rab15, Rab18, Rab1a, Rab1B, Rab21, Rab23, Rab27a, Rab27a GTPase, Rab27B, Rab2A, Rab2B, Rab30, Rab33A, Rab35, Rab36, Rab37, Rab38, Rab3A, Rab4a, Rab6A, Rab6B, Rab8, Rab8A, Rab9A, Rab9B, RabE, RabE1d, Rac, Rac GTPase, Rac-like GTP-binding protein 1, Rac1, Rac1 GTPase, Rac2, Rac3, Rac4, Rad, Rad GTPase, Ral GTPase, RalA, RalB, Ran, RAP, Rap GTPase, Rap1, Rap1 small GTPase, Rap1A, Rap1b, Rap2, Rap2 small GTPase, Rap2a, Rap2C, RAS, Ras GTPase, Ras homolog enriched in brain-like protein 1, Ras proximity 1, Ras related GTPase Rap, Ras-associated protein 1, Ras-like G-protein, Ras-like GTpase, Ras-related C3 botulinum toxin substrate 1, Ras-related protein rab-5, putative, Ras-related small GTPase, RAS1, RAS2, RasL10B, Rasl11a, Rem2, Rhb1, Rheb, Rheb GTPase, RHEBL1, Rho, Rho family small GTPase, Rho GTPase, RHO-1, Rho-GTPase, Rho-type GTPase, Rho-type small GTPase, Rho1, Rho1p, Rho3, Rho3p, RhoA, rhoA p21, RhoB, rhoB p20, RhoC, RhoGTPase, RhoH, ribosomal GTPase, ribosome-small-subunit-dependent GTPase A, RsgA, Ryh1, Ryh1 GTPase, Sar1, SdArf1, SdArf1-like, SdArf10, SdArf5, SdArf6, SdArl1, SdCdc42, SdK-Ras2, SdRab-like1, SdRab-like2, SdRab-like3, SdRab-like4, SdRab-like5, SdRab-like6, SdRab1-like, SdRab10, SdRab11, SdRab14, SdRab18, SdRab2, SdRab20-like, SdRab21, SdRab21-like, SdRab24, SdRab28, SdRab3, SdRab32, SdRab35, SdRab39, SdRab4, SdRab41/43, SdRab5, SdRab8, SdRac, SdRalA, SdRan, SdRap1, SdRap1-like, SdRheb, SdRho1, SdRho2, SdRho3, small G protein, small GTPase, small GTPase ARF6, small GTPase LIP1, small GTPase protein Rac-1, small GTPase Rab1, small GTPase Rab11b, small GTPase Rab2, small GTPase Rab21, small GTPase Rab27b, small GTPase Rac, small GTPase Rac-1, small GTPase Ral, small GTPase Ras, small GTPase Ras-related protein in brain 13, small GTPase Rho, small GTPase RhoA, small GTPase RhoV, small GTPase Rnd1, small GTPases Rab5, small GTPases Ral5, small nuclear GTPase Ran, small Rho GTPase, small Rho GTPase Rac1, small, monomeric G protein, small-GTPase, SmGTP, TbArl6, TcRABL4, vacuolar Rab GTPase, Wrch-1, YjeQ, Ypt1, Ypt1p, Ypt7, Ypt7p, YsxC

ECTree

     3 Hydrolases
         3.6 Acting on acid anhydrides
             3.6.5 Acting on GTP to facilitate cellular and subcellular movement
                3.6.5.2 small monomeric GTPase

Purification

Purification on EC 3.6.5.2 - small monomeric GTPase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
DEAE cellulose chromatography, heparin-Sepharose column chromatography, and Q-Sepharose column chromatography
-
glutathione Sepharose 4B column chromatography
-
glutathione-agarose bead chromatography and Superdex-75 gel filtration
-
glutathione-Sepharose 4B bead chromatography
-
glutathione-Sepharose bead chromatography
GppNHp-bound Rab27a in complex with the Rab27-binding domain of exophilin4/Slp2-a effector, glutathione Sepharose 4B resin chromatography and Superdex-75 gel filtration
-
GST-tagged Rheb by glutathione affinity chromatography, recombinant enzyme from HEK-293 cells by tandem affinity purification through IgG-binding and anti-Flag affinity chromatography
GTPase core domain overexpressed in Escherichia coli
-
GTPase domain of human Rheb recombinantly expressed in Escherichia coli
-
near homogeneity, recombinant enzyme, affinity chromatography
-
Q Sepharose column chromatography, ammonium sulfate precipitation, glutathione 4B-Sepharose bead chromatography, and Superdex 200 gel filtration
-
recombinant
-
recombinant enzyme from Escherichia coli strain BL21(DE3)
recombinant enzymes, affinity chromatography
recombinant glutathione S-transferase (GST)-Wrch- and hexa-histidine (His6)-tagged Wrch-1 protein from Escherichia coli
-
recombinant GST-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3)pLysS by glutathione affinity chromatography, the GST-tag is removed by thrombin cleavage, followed by heparin affinity chromatography, DnaK removal by ATP affinity chromatography, and dialysis
-
recombinant GST-tagged wild-type and mutant enzymes from HEK cells by glutathione affinity chromatography. Purification GST-TBCD with or withoout ARL2 from HEK cells results in the co-purification of different combinations of tubulins in novel complexes
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
recombinant His6-tagged enzyme from Escherichia coli strain Rosetta by nickel affinity chromatography and blue native PAGE
recombinant N-terminally GST-tagged wild-type and mutant enzymes from Escherichia coli Rosetta (DE3) by glutathione affinity chromatography
recombinant N-terminally His6-tagged TbArl6 by affinity chromatgraphy and gelfiltration
recombinant Rac1
recombinant Rac2
recombinant Rac3
recombinant wild-type and mutant Rab27a
-
the cytosolic G domain of psToc34 (lacking the C-terminal membrane anchor) is purified mainly in the GDP-bound form after recombinant protein production in Escherichia coli
to homogeneity, chromatography techniques
-
vacuole preparation
-