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3.6.5.2: small monomeric GTPase

This is an abbreviated version!
For detailed information about small monomeric GTPase, go to the full flat file.

Word Map on EC 3.6.5.2

Reaction

GTP
+
H2O
=
GDP
 +
phosphate

Synonyms

ADP-ribosylation factor 6, ADP-ribosylation factor-like protein 2, Alp41, Alp41/Arl2, ARD1, Arf 1, Arf 1-6, Arf-like 3 small GTPase, Arf6, ARL2, Arl6, Arl6/BBS3, Arl9, AtRopGEF12, Cdc42, Cdc42Hs, Der, Der GTPase, double Era-like protein, EC 3.6.1.47, EF-Tu, EhRab11B, elF-2, EmRas, FgCdc42, FgRac1, FgRHO1, FgRHO2, FgRHO3, FgRHO4, GCC185, Gem, GhRac1 GTPase, Gsp1p, GTP-phosphohydrolase, GTPase, GTPase Alp41/Arl2, GTPase TcRho1, GTPase Toc33, GTPase Toc33/34, guanine triphosphatase, guanosine 5'-triphosphatase, guanosine triphosphatase, H-Ras, K-Ras, Kir, Krev-1, M-Ras, monomeric G protein, monomeric GTPase, More, non-canonical Ras-like G protein, OsRac1, p21 ras, Pc-Rac1, PfG, PRA, Pra2, Pra3, Rab GTPase, Rab GTPase protein 5, Rab11, Rab13, Rab15, Rab18, Rab1a, Rab1B, Rab21, Rab23, Rab27a, Rab27a GTPase, Rab27B, Rab2A, Rab2B, Rab30, Rab33A, Rab35, Rab36, Rab37, Rab38, Rab3A, Rab4a, Rab6A, Rab6B, Rab8, Rab8A, Rab9A, Rab9B, RabE, RabE1d, Rac, Rac GTPase, Rac-like GTP-binding protein 1, Rac1, Rac1 GTPase, Rac2, Rac3, Rac4, Rad, Rad GTPase, Ral GTPase, RalA, RalB, Ran, RAP, Rap GTPase, Rap1, Rap1 small GTPase, Rap1A, Rap1b, Rap2, Rap2 small GTPase, Rap2a, Rap2C, RAS, Ras GTPase, Ras homolog enriched in brain-like protein 1, Ras proximity 1, Ras related GTPase Rap, Ras-associated protein 1, Ras-like G-protein, Ras-like GTpase, Ras-related C3 botulinum toxin substrate 1, Ras-related protein rab-5, putative, Ras-related small GTPase, RAS1, RAS2, RasL10B, Rasl11a, Rem2, Rhb1, Rheb, Rheb GTPase, RHEBL1, Rho, Rho family small GTPase, Rho GTPase, RHO-1, Rho-GTPase, Rho-type GTPase, Rho-type small GTPase, Rho1, Rho1p, Rho3, Rho3p, RhoA, rhoA p21, RhoB, rhoB p20, RhoC, RhoGTPase, RhoH, ribosomal GTPase, ribosome-small-subunit-dependent GTPase A, RsgA, Ryh1, Ryh1 GTPase, Sar1, SdArf1, SdArf1-like, SdArf10, SdArf5, SdArf6, SdArl1, SdCdc42, SdK-Ras2, SdRab-like1, SdRab-like2, SdRab-like3, SdRab-like4, SdRab-like5, SdRab-like6, SdRab1-like, SdRab10, SdRab11, SdRab14, SdRab18, SdRab2, SdRab20-like, SdRab21, SdRab21-like, SdRab24, SdRab28, SdRab3, SdRab32, SdRab35, SdRab39, SdRab4, SdRab41/43, SdRab5, SdRab8, SdRac, SdRalA, SdRan, SdRap1, SdRap1-like, SdRheb, SdRho1, SdRho2, SdRho3, small G protein, small GTPase, small GTPase ARF6, small GTPase LIP1, small GTPase protein Rac-1, small GTPase Rab1, small GTPase Rab11b, small GTPase Rab2, small GTPase Rab21, small GTPase Rab27b, small GTPase Rac, small GTPase Rac-1, small GTPase Ral, small GTPase Ras, small GTPase Ras-related protein in brain 13, small GTPase Rho, small GTPase RhoA, small GTPase RhoV, small GTPase Rnd1, small GTPases Rab5, small GTPases Ral5, small nuclear GTPase Ran, small Rho GTPase, small Rho GTPase Rac1, small, monomeric G protein, small-GTPase, SmGTP, TbArl6, TcRABL4, vacuolar Rab GTPase, Wrch-1, YjeQ, Ypt1, Ypt1p, Ypt7, Ypt7p, YsxC

ECTree

     3 Hydrolases
         3.6 Acting on acid anhydrides
             3.6.5 Acting on GTP to facilitate cellular and subcellular movement
                3.6.5.2 small monomeric GTPase

Cloned

Cloned on EC 3.6.5.2 - small monomeric GTPase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
analysis of rhoB mRNA level by semi-quantitative real-time RT-PCR
Cdc42, expression in Escherichia coli
-
coexpression of GFP-LC3 and either Sar1 wild-type or the mutants Sar1 H79G or Sar1 T39N in CHO cells, effect of overexpression of Sar1 and its mutants in the secretory pathway, overview. Overexpression of GFP-tagged Rab1b wild-type or mutant Rab1b in CHO cells. Overexpression of GFP-tagged Rab1b N121I mutant in Vero monkey cells and human HaCaT cells
-
complete coding sequence of ARL9, from testis, maps to chromosome 4q12
complete coding sequence of RASL11A, from prostate, maps to chromosome 13q12.2
determination of transcriptional expression profiles of monomeric GTPases, analysed by real-time quantitative reverse transcription-PCR
-
expressed in Escherichia coli
-
expressed in Escherichia coli BL21 cells
expressed in Escherichia coli BL21(DE3) cells
-
expressed in Escherichia coli BL21(DE3) LysS cells. Three crystal structures of Rab27b-GDP were solved in three different space groups: P6522, C2 and P212121
-
expressed in Escherichia coli BL21-Codon-Plus(DE3) cells, and in MDCKII and Flp-In-293 cells
-
expressed in Escherichia coli strain BL21(DE3)
expressed in Escherichia coli strain BL21(DE3)-LysS
-
expression in COS-7 cells
-
expression in COS7 cells
expression in Escherichia coli
expression in insect cell culture
-
expression in mammalian cell culture
-
expression in Sf9 cells
gene PfG, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree, RT-PCR enzyme expression analysis, recombinant expression of His6-tagged enzyme in Escherichia coli strain Rosetta, subcloning in Escherichia coli strain NEB 5-alpha
gene RAB13, semiquantitative RT-PCR and quantitative real-time PCR expression analysis
gene rac1, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, real-time PCR enzyme expression analysis
-
gene rad, recombinant expression of GST-tagged Rad in Escherichia coli
-
gene YPT1, recombinant expression of GST-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3)pLysS
-
genes rap1a and rap1b, genetic models
-
GTPase core domain is overexpressed in Escherichia coli
-
GTPase domain of human Rheb recombinantly expressed in Escherichia coli
-
heterologous expression of RhoA in Xenopus laevis oocytes. Expression of constitutively active RhoA in primary culture of spinal motoneurons also drastically reduces high-voltage-activated Ca2+ current amplitude
mutant enzymes Q47L and S29N are expressed in Arabidopsis thaliana
-
OsRac1, DNA and amino acid sequence determination and analysis, recombinant expression of N-terminally GST-tagged wild-type and mutant enzymes in Escherichia coli Rosetta (DE3), transient coexpression of OsRac1 andthe NADPH oxidase OsRbohB in Nicotiana benthamiana leaves enhancing ROS production
overexpression in Dictyostelium discoideum
-
overexpression of GhRac1 in Escherichia coli
-
Pra2 cDNA, expression in tobacco Bright Yellow 2 cells
-
Pra3 cDNA, expression in tobacco Bright Yellow 2 cells
-
production of Entamoeba histolytica transformants overexpressing EhRab11B
-
quantitative RT-PCR enzyme expression analysis, cotransfection of the NF-kappaB reporter with TLR2, TLR3, and TLR4, CD14, MD2 and Rap2a encoding plasmids into HEK-293 cells, and further treating the cells with the TLR ligands
Rab1A, genome-wide screening for human Rabs that are involved in Golgi morphology in HeLa-S3 cells using specific siRNAs
Rab1B, genome-wide screening for human Rabs that are involved in Golgi morphology in HeLa-S3 cells using specific siRNAs
Rab21 cDNA from K-562 cell, expression in HeLa cells CCL-2 and in hepatocellular carcinoma Hep3B cells
Rab2A, genome-wide screening for human Rabs that are involved in Golgi morphology in HeLa-S3 cells using specific siRNAs
Rab2B, genome-wide screening for human Rabs that are involved in Golgi morphology in HeLa-S3 cells using specific siRNAs
Rab6A, genome-wide screening for human Rabs that are involved in Golgi morphology in HeLa-S3 cells using specific siRNAs
Rab8B, genome-wide screening for human Rabs that are involved in Golgi morphology in HeLa-S3 cells using specific siRNAs
Rac1, expression in Escherichia coli as glutathione S-transferase fusion protein
Rac2, expression in Escherichia coli as glutathione S-transferase fusion protein
Rac3, expression in Escherichia coli as glutathione S-transferase fusion protein
recombinant expression of dual tagged Rheb in HEK-293 cells or of GST-tagged Rheb
recombinant expression of enzyme mutants FgRac1-G17V/D123A and FgCdc42-G15V/D121A in Saccharomyces cerevisiae strain AH109 in the yeast two-hybrid assay
recombinant expression of GST-tagged RapI protein
recombinant expression of GST-tagged RhoA wild-type (amino acids 1-193) in Escherichia coli
recombinant expression of H-Ras wild-type (amino acids 2-189) and H-Ras mutant Q61G (amino acids 2-189) in Escherichia coli
recombinant expression of HA- and GST-tagged wild-type and mutant enzymes in HEK cells, recombinant optimized expression of Arl2 in HeLa cells
recombinant expression of His6-tagged Alp41 in Escherichia coli, coexpression with activator Tbc1. HAT-tagged Tbc1 coimmunoprecipitates with GFP-tagged Alp41
recombinant expression of K-Ras wild-type (amino acids 2-189) in Escherichia coli
recombinant expression of N-terminally His6-tagged TbArl6
recombinant expression of Raichu-OsRac1 in rice protoplasts from strain C5924 with high efficiency
recombinant expression of the enzyme
recombinant expression of the enzyme in Escherichia coli
recombinant expression of the enzyme in Escherichia coli strain BL21(DE3)
recombinant expression of the His-tagged enzyme in Escherichia coli strain BL21(DE3)
T19N and G14V mutant rho-1 cDNA
-
wild-type and mutant Rab27a cDNA, expression in Escherichia coli BL21 and in 293T cells, expression of mutant Rab27a in the mouse melanocyte cell line melan-a
-