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3.5.2.9: 5-oxoprolinase (ATP-hydrolysing)

This is an abbreviated version!
For detailed information about 5-oxoprolinase (ATP-hydrolysing), go to the full flat file.

Word Map on EC 3.5.2.9

Reaction

ATP
+
5-oxo-L-proline
 + 2 H2O =
ADP
 +
phosphate
 +
L-glutamate

Synonyms

5-OPase, 5-oxo-L-prolinase, 5-oxoprolinase, 5OPase, ATP-dependent 5-oxoprolinase, FgOXP1, FgOXP2, FGSG_04902, FGSG_10203, L-pyroglutamate hydrolase, OPase, oplA, OPLAH, oxoprolinase, OXP1, PA4511, prokaryotic 5-oxoprolinase, PxpA, pxpA3, pxpB, pxpC, pyroglutamase, pyroglutamase (ATP-hydrolysing), pyroglutamate hydrolase, pyroglutamic hydrolase, SSO1667

ECTree

     3 Hydrolases
         3.5 Acting on carbon-nitrogen bonds, other than peptide bonds
             3.5.2 In cyclic amides
                3.5.2.9 5-oxoprolinase (ATP-hydrolysing)

Cloned

Cloned on EC 3.5.2.9 - 5-oxoprolinase (ATP-hydrolysing)

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
amino acid sequence determined by cDNA cloning, gene expressed in Escherichia coli
cDNA in pT7-7, subcloned into mammalian expression vector pRc/CMV, transfected into MCF7
-
expression in Saccharamyces cerevisiae, N-terminally His-tagged
gene FGSG_04902, phylogenetic analysis
gene FGSG_10203, sequence comparisons and phylogenetic analysis
gene pxpA3, phylogenetic analysis
genes pxpA, pxpB, and pxpC, comparative genomics, genetic structure, DNA and amino acid sequence determination and analysis, sequence comparisons, recombinant expression of His-tagged pxpA, and combined expression of N-terminally or C-terminally pxpB and pxpC, in Escherichia coli strain BL21(DE3) RIPL