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3.5.2.9: 5-oxoprolinase (ATP-hydrolysing)

This is an abbreviated version!
For detailed information about 5-oxoprolinase (ATP-hydrolysing), go to the full flat file.

Word Map on EC 3.5.2.9

Reaction

ATP
+
5-oxo-L-proline
+ 2 H2O =
ADP
+
phosphate
+
L-glutamate

Synonyms

5-OPase, 5-oxo-L-prolinase, 5-oxoprolinase, 5OPase, ATP-dependent 5-oxoprolinase, FgOXP1, FgOXP2, FGSG_04902, FGSG_10203, L-pyroglutamate hydrolase, OPase, oplA, OPLAH, oxoprolinase, OXP1, PA4511, prokaryotic 5-oxoprolinase, PxpA, pxpA3, pxpB, pxpC, pyroglutamase, pyroglutamase (ATP-hydrolysing), pyroglutamate hydrolase, pyroglutamic hydrolase, SSO1667

ECTree

     3 Hydrolases
         3.5 Acting on carbon-nitrogen bonds, other than peptide bonds
             3.5.2 In cyclic amides
                3.5.2.9 5-oxoprolinase (ATP-hydrolysing)

Storage Stability

Storage Stability on EC 3.5.2.9 - 5-oxoprolinase (ATP-hydrolysing)

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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-70°C, no loss of activity detected
-
-80°C, no activity lost for up to 1 week
-
0°C, stable for up to 2 months
-
4°C, 30 mM Tris-HCl buffer pH 7.8, 10 mM potassium chloride, 1 mM MgCl2, 5 mM 5-oxoproline, activity decreases to about 60% of its initial value after 2 days, 42% after 4 days and 40% after 5-6 days, addition of dithiothreitol leads to 104% of initial activity
-
4°C, loses progressively activity, 60% initial activity lost in 6 days, activity completely restored upon addition of 5 mM dithiothreitol
-
4°C, up to 30 h without decrease in activity
-