3.5.2.9: 5-oxoprolinase (ATP-hydrolysing)
This is an abbreviated version!
For detailed information about 5-oxoprolinase (ATP-hydrolysing), go to the full flat file.
Word Map on EC 3.5.2.9
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3.5.2.9
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5-oxoproline
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gamma-glutamyl
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5-oxoprolinuria
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l-2-oxothiazolidine-4-carboxylate
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medicine
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cyclotransferase
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decyclization
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gamma-glutamyl-cysteine
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atp-hydrolyzing
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meister
- 3.5.2.9
- 5-oxoproline
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gamma-glutamyl
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5-oxoprolinuria
- l-2-oxothiazolidine-4-carboxylate
- medicine
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cyclotransferase
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decyclization
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gamma-glutamyl-cysteine
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atp-hydrolyzing
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meister
Reaction
Synonyms
5-OPase, 5-oxo-L-prolinase, 5-oxoprolinase, 5OPase, ATP-dependent 5-oxoprolinase, FgOXP1, FgOXP2, FGSG_04902, FGSG_10203, L-pyroglutamate hydrolase, OPase, oplA, OPLAH, oxoprolinase, OXP1, PA4511, prokaryotic 5-oxoprolinase, PxpA, pxpA3, pxpB, pxpC, pyroglutamase, pyroglutamase (ATP-hydrolysing), pyroglutamate hydrolase, pyroglutamic hydrolase, SSO1667
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General Information
General Information on EC 3.5.2.9 - 5-oxoprolinase (ATP-hydrolysing)
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evolution
malfunction
metabolism
physiological function
additional information
comparative analysis of prokaryotic genomes shows that the gene encoding pyroglutamyl peptidase, which removes N-terminal 5-oxoproline residues, clusters in diverse genomes with genes specifying homologs of a fungal lactamase (renamed prokaryotic 5-oxoprolinase A, pxpA) and homologs of allophanate hydrolase subunits (renamed pxpB and pxpC). 5-Oxoproline is a major universal metabolite damage product and its disposal systems are common in all domains of life
evolution
detailed phylogenetic analysis of 5-oxoprolinases, overview
evolution
phylogenetic analysis suggests a relationship between taxonomic grouping and PxpA oligomerization
evolution
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phylogenetic analysis suggests a relationship between taxonomic grouping and PxpA oligomerization
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evolution
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comparative analysis of prokaryotic genomes shows that the gene encoding pyroglutamyl peptidase, which removes N-terminal 5-oxoproline residues, clusters in diverse genomes with genes specifying homologs of a fungal lactamase (renamed prokaryotic 5-oxoprolinase A, pxpA) and homologs of allophanate hydrolase subunits (renamed pxpB and pxpC). 5-Oxoproline is a major universal metabolite damage product and its disposal systems are common in all domains of life
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evolution
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phylogenetic analysis suggests a relationship between taxonomic grouping and PxpA oligomerization
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evolution
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phylogenetic analysis suggests a relationship between taxonomic grouping and PxpA oligomerization
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evolution
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phylogenetic analysis suggests a relationship between taxonomic grouping and PxpA oligomerization
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evolution
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phylogenetic analysis suggests a relationship between taxonomic grouping and PxpA oligomerization
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evolution
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phylogenetic analysis suggests a relationship between taxonomic grouping and PxpA oligomerization
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evolution
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phylogenetic analysis suggests a relationship between taxonomic grouping and PxpA oligomerization
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inherited 5-oxoprolinase deficiency is a rare 5-oxoprolinase deficiency is an extremely rare disorder of the gamma-glutamyl cycle characterised by 5-oxoprolinuria, heterogeneity of the clinical presentation which ranges from normal to significant neurological involvement, genotype-phenotype correlation, phenotypes, overview
malfunction
inherited 5-oxoprolinase deficiency is a rare inborn condition characterised by 5-oxoprolinuria. Three enzyme mutations are involved: p.H870Pfs in a homozygous state, which results in a truncated protein, and two heterozygous missense changes, S323R and V1089I
malfunction
5-oxo-L-proline is a competitive inhibitor for glutamate transport in Sulfolobus solfataricus. The growth inhibiting effect of 5-oxo-L-proline on the cell culture is not only due to the loss of available carbon, because its addition to a growing culture can lead to cell death
malfunction
deletion of FgOXP1 or FgOXP2 in Fusarium graminearum leads to significant defects in its virulence on wheat, likely caused by an observed decreased deoxynivalenol (DON, a mycotoxin) production in the gene deletion mutant strains. DON is one of the best characterized virulence factors of Fusarium graminearum. The FgOXP2 deletion mutant strains are also defective in conidiation and sexual reproduction while the FgOXP1 deletion mutant strains are normal for those phenotypes. Double deletion of FgOXP1 and FgOXP2 leads to more severe defects in conidiation, DON production and virulence on plants, suggesting that both FgOXP1 and FgOXP2 play a role in fungal development and plant colonization. Although transformation of the enzyme from Magnaporthe oryzae wild-type strain 70-15, MoOXP1, into DELTAFgoxp1 is able to complement DELTAFgoxp1, transformation of MoOXP1 into DELTAFgoxp2 fails to restore its defects in sexual development, DON production, and pathogenicity. Defects noticed in the gene deletion mutant strains of 5-oxoprolinase in Fusarium graminearum are caused by the affected gamma-glutamyl cycle, phenotypes, overview
malfunction
deletion of FgOXP1 or FgOXP2 in Fusarium graminearum leads to significant defects in its virulence on wheat, likely caused by an observed decreased deoxynivalenol (DON, a mycotoxin) production in the gene deletion mutant strains. DON is one of the best characterized virulence factors of Fusarium graminearum. The FgOXP2 deletion mutant strains are also defective in conidiation and sexual reproduction while the FgOXP1 deletion mutant strains are normal for those phenotypes. Double deletion of FgOXP1 and FgOXP2 leads to more severe defects in conidiation, DON production and virulence on plants, suggesting that both FgOXP1 and FgOXP2 play a role in fungal development and plant colonization. Although transformation of the enzyme from Magnaporthe oryzae wild-type strain 70-15, MoOXP1, into DELTAFgoxp1, transformation of MoOXP1 into DELTAFgoxp2 fails to restore its defects in sexual development, DON production, and pathogenicity. Defects noticed in the gene deletion mutant strains of 5-oxoprolinase in Fusarium graminearum are caused by the affected gamma-glutamyl cycle, phenotypes, overview
malfunction
inactivation of Bacillus subtilis pxpA, pxpB, or pxpC genes slows growth, causes 5-oxoproline accumulation in cells and medium, and prevents use of 5-oxoproline as a nitrogen source. ATP-dependent 5-oxoprolinase activity disappears when pxpA, pxpB, or pxpC is inactivated
malfunction
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5-oxo-L-proline is a competitive inhibitor for glutamate transport in Sulfolobus solfataricus. The growth inhibiting effect of 5-oxo-L-proline on the cell culture is not only due to the loss of available carbon, because its addition to a growing culture can lead to cell death
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malfunction
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inactivation of Bacillus subtilis pxpA, pxpB, or pxpC genes slows growth, causes 5-oxoproline accumulation in cells and medium, and prevents use of 5-oxoproline as a nitrogen source. ATP-dependent 5-oxoprolinase activity disappears when pxpA, pxpB, or pxpC is inactivated
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malfunction
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5-oxo-L-proline is a competitive inhibitor for glutamate transport in Sulfolobus solfataricus. The growth inhibiting effect of 5-oxo-L-proline on the cell culture is not only due to the loss of available carbon, because its addition to a growing culture can lead to cell death
-
malfunction
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5-oxo-L-proline is a competitive inhibitor for glutamate transport in Sulfolobus solfataricus. The growth inhibiting effect of 5-oxo-L-proline on the cell culture is not only due to the loss of available carbon, because its addition to a growing culture can lead to cell death
-
malfunction
-
5-oxo-L-proline is a competitive inhibitor for glutamate transport in Sulfolobus solfataricus. The growth inhibiting effect of 5-oxo-L-proline on the cell culture is not only due to the loss of available carbon, because its addition to a growing culture can lead to cell death
-
malfunction
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deletion of FgOXP1 or FgOXP2 in Fusarium graminearum leads to significant defects in its virulence on wheat, likely caused by an observed decreased deoxynivalenol (DON, a mycotoxin) production in the gene deletion mutant strains. DON is one of the best characterized virulence factors of Fusarium graminearum. The FgOXP2 deletion mutant strains are also defective in conidiation and sexual reproduction while the FgOXP1 deletion mutant strains are normal for those phenotypes. Double deletion of FgOXP1 and FgOXP2 leads to more severe defects in conidiation, DON production and virulence on plants, suggesting that both FgOXP1 and FgOXP2 play a role in fungal development and plant colonization. Although transformation of the enzyme from Magnaporthe oryzae wild-type strain 70-15, MoOXP1, into DELTAFgoxp1, transformation of MoOXP1 into DELTAFgoxp2 fails to restore its defects in sexual development, DON production, and pathogenicity. Defects noticed in the gene deletion mutant strains of 5-oxoprolinase in Fusarium graminearum are caused by the affected gamma-glutamyl cycle, phenotypes, overview
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malfunction
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deletion of FgOXP1 or FgOXP2 in Fusarium graminearum leads to significant defects in its virulence on wheat, likely caused by an observed decreased deoxynivalenol (DON, a mycotoxin) production in the gene deletion mutant strains. DON is one of the best characterized virulence factors of Fusarium graminearum. The FgOXP2 deletion mutant strains are also defective in conidiation and sexual reproduction while the FgOXP1 deletion mutant strains are normal for those phenotypes. Double deletion of FgOXP1 and FgOXP2 leads to more severe defects in conidiation, DON production and virulence on plants, suggesting that both FgOXP1 and FgOXP2 play a role in fungal development and plant colonization. Although transformation of the enzyme from Magnaporthe oryzae wild-type strain 70-15, MoOXP1, into DELTAFgoxp1 is able to complement DELTAFgoxp1, transformation of MoOXP1 into DELTAFgoxp2 fails to restore its defects in sexual development, DON production, and pathogenicity. Defects noticed in the gene deletion mutant strains of 5-oxoprolinase in Fusarium graminearum are caused by the affected gamma-glutamyl cycle, phenotypes, overview
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the enzyme is involved in the gamma-glutamyl cycle, a six-enzyme cycle that represents the primary pathway for glutathione synthesis and degradation
metabolism
comparison of the effect of 5-oxoproline on the growth of Sulfolobus solfataricus and the closely related crenarchaeon Sulfolobus acidocaldarius. Sulfolobus solfataricus shows intracellular accumulation of 5-oxoproline and crude cell extract assays show a less effective degradation of 5-oxoproline. Sulfolobus acidocaldarius seems to be less versatile regarding carbohydrates and prefers peptidolytic growth compared to Sulfolobus solfataricus. Concludingly, Sulfolobus acidocaldarius exhibits a more efficient utilization of 5-oxoproline and is not inhibited by this compound, making it a better candidate for applications with glutamate-containing media at high temperatures
metabolism
comparison of the effect of 5-oxoproline on the growth of Sulfolobus solfataricus and the closely related crenarchaeon Sulfolobus acidocaldarius. Sulfolobus solfataricus shows intracellular accumulation of 5-oxoproline and crude cell extract assays show a less effective degradation of 5-oxoproline. Sulfolobus acidocaldarius seems to be less versatile regarding carbohydrates and prefers peptidolytic growth compared to Sulfolobus solfataricus. Concludingly, Sulfolobus acidocaldarius exhibits a more efficient utilization of 5-oxoproline and is not inhibited by this compound, making it a better candidate for applications with glutamate-containing media at high temperatures
metabolism
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comparison of the effect of 5-oxoproline on the growth of Sulfolobus solfataricus and the closely related crenarchaeon Sulfolobus acidocaldarius. Sulfolobus solfataricus shows intracellular accumulation of 5-oxoproline and crude cell extract assays show a less effective degradation of 5-oxoproline. Sulfolobus acidocaldarius seems to be less versatile regarding carbohydrates and prefers peptidolytic growth compared to Sulfolobus solfataricus. Concludingly, Sulfolobus acidocaldarius exhibits a more efficient utilization of 5-oxoproline and is not inhibited by this compound, making it a better candidate for applications with glutamate-containing media at high temperatures
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metabolism
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comparison of the effect of 5-oxoproline on the growth of Sulfolobus solfataricus and the closely related crenarchaeon Sulfolobus acidocaldarius. Sulfolobus solfataricus shows intracellular accumulation of 5-oxoproline and crude cell extract assays show a less effective degradation of 5-oxoproline. Sulfolobus acidocaldarius seems to be less versatile regarding carbohydrates and prefers peptidolytic growth compared to Sulfolobus solfataricus. Concludingly, Sulfolobus acidocaldarius exhibits a more efficient utilization of 5-oxoproline and is not inhibited by this compound, making it a better candidate for applications with glutamate-containing media at high temperatures
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metabolism
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comparison of the effect of 5-oxoproline on the growth of Sulfolobus solfataricus and the closely related crenarchaeon Sulfolobus acidocaldarius. Sulfolobus solfataricus shows intracellular accumulation of 5-oxoproline and crude cell extract assays show a less effective degradation of 5-oxoproline. Sulfolobus acidocaldarius seems to be less versatile regarding carbohydrates and prefers peptidolytic growth compared to Sulfolobus solfataricus. Concludingly, Sulfolobus acidocaldarius exhibits a more efficient utilization of 5-oxoproline and is not inhibited by this compound, making it a better candidate for applications with glutamate-containing media at high temperatures
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metabolism
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comparison of the effect of 5-oxoproline on the growth of Sulfolobus solfataricus and the closely related crenarchaeon Sulfolobus acidocaldarius. Sulfolobus solfataricus shows intracellular accumulation of 5-oxoproline and crude cell extract assays show a less effective degradation of 5-oxoproline. Sulfolobus acidocaldarius seems to be less versatile regarding carbohydrates and prefers peptidolytic growth compared to Sulfolobus solfataricus. Concludingly, Sulfolobus acidocaldarius exhibits a more efficient utilization of 5-oxoproline and is not inhibited by this compound, making it a better candidate for applications with glutamate-containing media at high temperatures
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metabolism
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comparison of the effect of 5-oxoproline on the growth of Sulfolobus solfataricus and the closely related crenarchaeon Sulfolobus acidocaldarius. Sulfolobus solfataricus shows intracellular accumulation of 5-oxoproline and crude cell extract assays show a less effective degradation of 5-oxoproline. Sulfolobus acidocaldarius seems to be less versatile regarding carbohydrates and prefers peptidolytic growth compared to Sulfolobus solfataricus. Concludingly, Sulfolobus acidocaldarius exhibits a more efficient utilization of 5-oxoproline and is not inhibited by this compound, making it a better candidate for applications with glutamate-containing media at high temperatures
-
metabolism
-
comparison of the effect of 5-oxoproline on the growth of Sulfolobus solfataricus and the closely related crenarchaeon Sulfolobus acidocaldarius. Sulfolobus solfataricus shows intracellular accumulation of 5-oxoproline and crude cell extract assays show a less effective degradation of 5-oxoproline. Sulfolobus acidocaldarius seems to be less versatile regarding carbohydrates and prefers peptidolytic growth compared to Sulfolobus solfataricus. Concludingly, Sulfolobus acidocaldarius exhibits a more efficient utilization of 5-oxoproline and is not inhibited by this compound, making it a better candidate for applications with glutamate-containing media at high temperatures
-
metabolism
-
comparison of the effect of 5-oxoproline on the growth of Sulfolobus solfataricus and the closely related crenarchaeon Sulfolobus acidocaldarius. Sulfolobus solfataricus shows intracellular accumulation of 5-oxoproline and crude cell extract assays show a less effective degradation of 5-oxoproline. Sulfolobus acidocaldarius seems to be less versatile regarding carbohydrates and prefers peptidolytic growth compared to Sulfolobus solfataricus. Concludingly, Sulfolobus acidocaldarius exhibits a more efficient utilization of 5-oxoproline and is not inhibited by this compound, making it a better candidate for applications with glutamate-containing media at high temperatures
-
metabolism
-
comparison of the effect of 5-oxoproline on the growth of Sulfolobus solfataricus and the closely related crenarchaeon Sulfolobus acidocaldarius. Sulfolobus solfataricus shows intracellular accumulation of 5-oxoproline and crude cell extract assays show a less effective degradation of 5-oxoproline. Sulfolobus acidocaldarius seems to be less versatile regarding carbohydrates and prefers peptidolytic growth compared to Sulfolobus solfataricus. Concludingly, Sulfolobus acidocaldarius exhibits a more efficient utilization of 5-oxoproline and is not inhibited by this compound, making it a better candidate for applications with glutamate-containing media at high temperatures
-
metabolism
-
comparison of the effect of 5-oxoproline on the growth of Sulfolobus solfataricus and the closely related crenarchaeon Sulfolobus acidocaldarius. Sulfolobus solfataricus shows intracellular accumulation of 5-oxoproline and crude cell extract assays show a less effective degradation of 5-oxoproline. Sulfolobus acidocaldarius seems to be less versatile regarding carbohydrates and prefers peptidolytic growth compared to Sulfolobus solfataricus. Concludingly, Sulfolobus acidocaldarius exhibits a more efficient utilization of 5-oxoproline and is not inhibited by this compound, making it a better candidate for applications with glutamate-containing media at high temperatures
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comparison of the growth of 5-oxoprolinase deletion strains with wild-type strains in a variety of growth conditions does not yield any discernible phenotypes. Cells of glutamate auxotroph aco1D strain expressing OXP1 are able to grow on 5-oxoproline, although the growth is very slow
physiological function
5-oxoprolinase catalyses the ATP-dependent conversion of 5-oxoproline to glutamate
physiological function
5-oxoprolinase catalyses the ATP-dependent conversion of 5-oxoproline, i.e. pyroglutamate, to glutamate
physiological function
5-oxoprolinase is required for conidiation, sexual reproduction, virulence, and deoxynivalenol production of Fusarium graminearum. Both isozymes FgOXP1 and FgOXP2 play a role in fungal development and plant colonization. FgOXP1 and FgOXP2 function differentially in deoxynivalenol DON production and in sexual reproduction of Fusarium graminearum, overview
physiological function
5-oxoprolinase is required for conidiation, sexual reproduction, virulence, and deoxynivalenol production of Fusarium graminearum. Both isozymes FgOXP1 and FgOXP2 play a role in fungal development and plant colonization. FgOXP1 and FgOXP2 function differentially in deoxynivalenol DON production and in sexual reproduction of Fusarium graminearum, overview. A clearly much stronger role for Oxp2 in perithecia and ascus formation
physiological function
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5-oxoprolinase catalyses the ATP-dependent conversion of 5-oxoproline, i.e. pyroglutamate, to glutamate
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physiological function
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5-oxoprolinase catalyses the ATP-dependent conversion of 5-oxoproline to glutamate
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physiological function
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5-oxoprolinase catalyses the ATP-dependent conversion of 5-oxoproline, i.e. pyroglutamate, to glutamate
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physiological function
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5-oxoprolinase catalyses the ATP-dependent conversion of 5-oxoproline, i.e. pyroglutamate, to glutamate
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physiological function
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5-oxoprolinase catalyses the ATP-dependent conversion of 5-oxoproline to glutamate
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physiological function
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5-oxoprolinase catalyses the ATP-dependent conversion of 5-oxoproline, i.e. pyroglutamate, to glutamate
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physiological function
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5-oxoprolinase is required for conidiation, sexual reproduction, virulence, and deoxynivalenol production of Fusarium graminearum. Both isozymes FgOXP1 and FgOXP2 play a role in fungal development and plant colonization. FgOXP1 and FgOXP2 function differentially in deoxynivalenol DON production and in sexual reproduction of Fusarium graminearum, overview. A clearly much stronger role for Oxp2 in perithecia and ascus formation
-
physiological function
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5-oxoprolinase is required for conidiation, sexual reproduction, virulence, and deoxynivalenol production of Fusarium graminearum. Both isozymes FgOXP1 and FgOXP2 play a role in fungal development and plant colonization. FgOXP1 and FgOXP2 function differentially in deoxynivalenol DON production and in sexual reproduction of Fusarium graminearum, overview
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physiological function
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5-oxoprolinase catalyses the ATP-dependent conversion of 5-oxoproline to glutamate
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physiological function
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5-oxoprolinase catalyses the ATP-dependent conversion of 5-oxoproline to glutamate
-
physiological function
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5-oxoprolinase catalyses the ATP-dependent conversion of 5-oxoproline to glutamate
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glutamate is spontaneously converted into 5-oxoproline in a pH range of pH 2 to 3.5 and at temperatures above room temperature. This makes many thermoacidophiles, like, for example, Sulfolobus solfataricus, less suitable for a number of biotechnological approaches due to 5-oxoproline-induced growth restriction
additional information
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glutamate is spontaneously converted into 5-oxoproline in a pH range of pH 2 to 3.5 and at temperatures above room temperature. This makes many thermoacidophiles, like, for example, Sulfolobus solfataricus, less suitable for a number of biotechnological approaches due to 5-oxoproline-induced growth restriction
additional information
glutamate is spontaneously converted into 5-oxoproline in a pH range of pH 2 to 3.5 and at temperatures above room temperature. This makes many thermoacidophiles, like, for example, Sulfolobus solfataricus, less suitable for a number of biotechnological approaches due to pyroglutamate-induced growth restriction
additional information
-
glutamate is spontaneously converted into 5-oxoproline in a pH range of pH 2 to 3.5 and at temperatures above room temperature. This makes many thermoacidophiles, like, for example, Sulfolobus solfataricus, less suitable for a number of biotechnological approaches due to pyroglutamate-induced growth restriction
additional information
neither PxpA nor PxpBC alone have detectable OPase activity, only combined in equimolar amounts, they show ATP-dependent OPase activity. The activity is highly unstable, kinetic constants cannot be determined
additional information
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neither PxpA nor PxpBC alone have detectable OPase activity, only combined in equimolar amounts, they show ATP-dependent OPase activity. The activity is highly unstable, kinetic constants cannot be determined
additional information
subunit A, PxpA, associates with the subunit B and C complex, PxpBC, to form a functional 5-oxoprolinase enzyme for conversion of 5-oxoproline to L-glutamate. PxpBC catalyses the first step of the reaction, which is phosphorylation of 5-oxoproline. PxpA is involved in the last two steps of the reaction: decyclization of the labile phosphorylated 5-oxoproline to the equally labile gamma-glutamylphosphate, and subsequent dephosphorylation to L-glutamate. Structural bioinformatics analysis of four putative PxpA structures reveal that PxpA adopts a non-canonical TIM barrel fold with well-characterized TIM barrel enzyme features. Structure-function relationships of 5-oxoprolinase subunit A, overview. PxpA forms a tunnel upon ligand binding, thus suggesting that the PxpABC complex employs the mechanism of substrate channeling to protect labile intermediates. Active site structure of PxpA
additional information
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subunit A, PxpA, associates with the subunit B and C complex, PxpBC, to form a functional 5-oxoprolinase enzyme for conversion of 5-oxoproline to L-glutamate. PxpBC catalyses the first step of the reaction, which is phosphorylation of 5-oxoproline. PxpA is involved in the last two steps of the reaction: decyclization of the labile phosphorylated 5-oxoproline to the equally labile gamma-glutamylphosphate, and subsequent dephosphorylation to L-glutamate. Structural bioinformatics analysis of four putative PxpA structures reveal that PxpA adopts a non-canonical TIM barrel fold with well-characterized TIM barrel enzyme features. Structure-function relationships of 5-oxoprolinase subunit A, overview. PxpA forms a tunnel upon ligand binding, thus suggesting that the PxpABC complex employs the mechanism of substrate channeling to protect labile intermediates. Active site structure of PxpA
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additional information
-
glutamate is spontaneously converted into 5-oxoproline in a pH range of pH 2 to 3.5 and at temperatures above room temperature. This makes many thermoacidophiles, like, for example, Sulfolobus solfataricus, less suitable for a number of biotechnological approaches due to pyroglutamate-induced growth restriction
-
additional information
-
neither PxpA nor PxpBC alone have detectable OPase activity, only combined in equimolar amounts, they show ATP-dependent OPase activity. The activity is highly unstable, kinetic constants cannot be determined
-
additional information
-
glutamate is spontaneously converted into 5-oxoproline in a pH range of pH 2 to 3.5 and at temperatures above room temperature. This makes many thermoacidophiles, like, for example, Sulfolobus solfataricus, less suitable for a number of biotechnological approaches due to 5-oxoproline-induced growth restriction
-
additional information
-
subunit A, PxpA, associates with the subunit B and C complex, PxpBC, to form a functional 5-oxoprolinase enzyme for conversion of 5-oxoproline to L-glutamate. PxpBC catalyses the first step of the reaction, which is phosphorylation of 5-oxoproline. PxpA is involved in the last two steps of the reaction: decyclization of the labile phosphorylated 5-oxoproline to the equally labile gamma-glutamylphosphate, and subsequent dephosphorylation to L-glutamate. Structural bioinformatics analysis of four putative PxpA structures reveal that PxpA adopts a non-canonical TIM barrel fold with well-characterized TIM barrel enzyme features. Structure-function relationships of 5-oxoprolinase subunit A, overview. PxpA forms a tunnel upon ligand binding, thus suggesting that the PxpABC complex employs the mechanism of substrate channeling to protect labile intermediates. Active site structure of PxpA
-
additional information
-
subunit A, PxpA, associates with the subunit B and C complex, PxpBC, to form a functional 5-oxoprolinase enzyme for conversion of 5-oxoproline to L-glutamate. PxpBC catalyses the first step of the reaction, which is phosphorylation of 5-oxoproline. PxpA is involved in the last two steps of the reaction: decyclization of the labile phosphorylated 5-oxoproline to the equally labile gamma-glutamylphosphate, and subsequent dephosphorylation to L-glutamate. Structural bioinformatics analysis of four putative PxpA structures reveal that PxpA adopts a non-canonical TIM barrel fold with well-characterized TIM barrel enzyme features. Structure-function relationships of 5-oxoprolinase subunit A, overview. PxpA forms a tunnel upon ligand binding, thus suggesting that the PxpABC complex employs the mechanism of substrate channeling to protect labile intermediates. Active site structure of PxpA
-
additional information
-
glutamate is spontaneously converted into 5-oxoproline in a pH range of pH 2 to 3.5 and at temperatures above room temperature. This makes many thermoacidophiles, like, for example, Sulfolobus solfataricus, less suitable for a number of biotechnological approaches due to pyroglutamate-induced growth restriction
-
additional information
-
subunit A, PxpA, associates with the subunit B and C complex, PxpBC, to form a functional 5-oxoprolinase enzyme for conversion of 5-oxoproline to L-glutamate. PxpBC catalyses the first step of the reaction, which is phosphorylation of 5-oxoproline. PxpA is involved in the last two steps of the reaction: decyclization of the labile phosphorylated 5-oxoproline to the equally labile gamma-glutamylphosphate, and subsequent dephosphorylation to L-glutamate. Structural bioinformatics analysis of four putative PxpA structures reveal that PxpA adopts a non-canonical TIM barrel fold with well-characterized TIM barrel enzyme features. Structure-function relationships of 5-oxoprolinase subunit A, overview. PxpA forms a tunnel upon ligand binding, thus suggesting that the PxpABC complex employs the mechanism of substrate channeling to protect labile intermediates. Active site structure of PxpA
-
additional information
-
subunit A, PxpA, associates with the subunit B and C complex, PxpBC, to form a functional 5-oxoprolinase enzyme for conversion of 5-oxoproline to L-glutamate. PxpBC catalyses the first step of the reaction, which is phosphorylation of 5-oxoproline. PxpA is involved in the last two steps of the reaction: decyclization of the labile phosphorylated 5-oxoproline to the equally labile gamma-glutamylphosphate, and subsequent dephosphorylation to L-glutamate. Structural bioinformatics analysis of four putative PxpA structures reveal that PxpA adopts a non-canonical TIM barrel fold with well-characterized TIM barrel enzyme features. Structure-function relationships of 5-oxoprolinase subunit A, overview. PxpA forms a tunnel upon ligand binding, thus suggesting that the PxpABC complex employs the mechanism of substrate channeling to protect labile intermediates. Active site structure of PxpA
-
additional information
-
subunit A, PxpA, associates with the subunit B and C complex, PxpBC, to form a functional 5-oxoprolinase enzyme for conversion of 5-oxoproline to L-glutamate. PxpBC catalyses the first step of the reaction, which is phosphorylation of 5-oxoproline. PxpA is involved in the last two steps of the reaction: decyclization of the labile phosphorylated 5-oxoproline to the equally labile gamma-glutamylphosphate, and subsequent dephosphorylation to L-glutamate. Structural bioinformatics analysis of four putative PxpA structures reveal that PxpA adopts a non-canonical TIM barrel fold with well-characterized TIM barrel enzyme features. Structure-function relationships of 5-oxoprolinase subunit A, overview. PxpA forms a tunnel upon ligand binding, thus suggesting that the PxpABC complex employs the mechanism of substrate channeling to protect labile intermediates. Active site structure of PxpA
-
additional information
-
glutamate is spontaneously converted into 5-oxoproline in a pH range of pH 2 to 3.5 and at temperatures above room temperature. This makes many thermoacidophiles, like, for example, Sulfolobus solfataricus, less suitable for a number of biotechnological approaches due to pyroglutamate-induced growth restriction
-
additional information
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glutamate is spontaneously converted into 5-oxoproline in a pH range of pH 2 to 3.5 and at temperatures above room temperature. This makes many thermoacidophiles, like, for example, Sulfolobus solfataricus, less suitable for a number of biotechnological approaches due to 5-oxoproline-induced growth restriction
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additional information
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glutamate is spontaneously converted into 5-oxoproline in a pH range of pH 2 to 3.5 and at temperatures above room temperature. This makes many thermoacidophiles, like, for example, Sulfolobus solfataricus, less suitable for a number of biotechnological approaches due to pyroglutamate-induced growth restriction
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additional information
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subunit A, PxpA, associates with the subunit B and C complex, PxpBC, to form a functional 5-oxoprolinase enzyme for conversion of 5-oxoproline to L-glutamate. PxpBC catalyses the first step of the reaction, which is phosphorylation of 5-oxoproline. PxpA is involved in the last two steps of the reaction: decyclization of the labile phosphorylated 5-oxoproline to the equally labile gamma-glutamylphosphate, and subsequent dephosphorylation to L-glutamate. Structural bioinformatics analysis of four putative PxpA structures reveal that PxpA adopts a non-canonical TIM barrel fold with well-characterized TIM barrel enzyme features. Structure-function relationships of 5-oxoprolinase subunit A, overview. PxpA forms a tunnel upon ligand binding, thus suggesting that the PxpABC complex employs the mechanism of substrate channeling to protect labile intermediates. Active site structure of PxpA
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additional information
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glutamate is spontaneously converted into 5-oxoproline in a pH range of pH 2 to 3.5 and at temperatures above room temperature. This makes many thermoacidophiles, like, for example, Sulfolobus solfataricus, less suitable for a number of biotechnological approaches due to 5-oxoproline-induced growth restriction
-
additional information
-
glutamate is spontaneously converted into 5-oxoproline in a pH range of pH 2 to 3.5 and at temperatures above room temperature. This makes many thermoacidophiles, like, for example, Sulfolobus solfataricus, less suitable for a number of biotechnological approaches due to 5-oxoproline-induced growth restriction
-
additional information
-
glutamate is spontaneously converted into 5-oxoproline in a pH range of pH 2 to 3.5 and at temperatures above room temperature. This makes many thermoacidophiles, like, for example, Sulfolobus solfataricus, less suitable for a number of biotechnological approaches due to 5-oxoproline-induced growth restriction
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