Any feedback?
Please rate this page
(all_enzymes.php)
(0/150)

BRENDA support

3.5.1.87: N-carbamoyl-L-amino-acid hydrolase

This is an abbreviated version!
For detailed information about N-carbamoyl-L-amino-acid hydrolase, go to the full flat file.

Word Map on EC 3.5.1.87

Reaction

an N-carbamoyl-L-2-amino acid (a 2-ureido carboxylate)
+
H2O
=
an L-2-amino acid
 +
NH3
 +
CO2

Synonyms

ADS79_04835, AtcC, BsLcar, carbamoylated amino acid carbamoylase, hyuC, immobilized L-N-carbamoylase, L-carbamoylase, L-methionine-N-carbamoylase, L-N-carbamoylamino acid aminohydrolase, L-N-carbamoylase, L-NCC amidohydrolase, Lnc, LNCA, N-carbamoyl-amino-acid amidohydrolase, N-carbamoyl-L-alpha-amino acid amidohydrolase, N-carbamoyl-L-amino acid amidohydrolase, N-carbamoyl-L-amino-acid amidohydrolase, N-carbamoyl-L-amino-acid hydrolase, N-carbamoyl-L-cysteine amidohydrolase, N-carbamoyl-L-cysteine-acid amidohydrolase, N-carbamyl-L-amino acid amidohydrolase, NCC amidohydrolase, SmLcar

ECTree

     3 Hydrolases
         3.5 Acting on carbon-nitrogen bonds, other than peptide bonds
             3.5.1 In linear amides
                3.5.1.87 N-carbamoyl-L-amino-acid hydrolase

Cloned

Cloned on EC 3.5.1.87 - N-carbamoyl-L-amino-acid hydrolase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
Asp6 and His6-tagged enzyme, expression in Escherichia coli
-
expressed in Escherichia coli
expressed in Escherichia coli BL21
expression in Escherichia coli
gene atcC, organized in the L-cysteine synthesis gene cluster, DNA and amino acid sequence determination and analysis, recombinant expression in Escherichia coli
gene Bslcar, recombinant expression
gene Bslcar, recombinant overexpression in Escherichia coli strain BL21 using the pJAVI80Rha plasmid
gene lnc, recombinant expression of His-tagged enzyme in Escherichia coli
genomic library screening, DNA and amino acid sequence determination and analysis, genotyping and phylogenetic analysis
L-N-carbamoylase gene of Sinorhizobium meliloti was cloned into the pBluescipt II SK (+) vector for expression in Escherichia coli
-
NCC amidohydrolase gene is cloned into DH5alpha and pet15b vectors for protein expression in Escherichia coli
-
the L-N-carbamoylase gene is amplified by PCR and cloned into the pBSK vector for expression of the protein in Escherichia coli BL21(DE3) cells
-