Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Co2+ | 2 mM, specific activity 2.27, relative activity 339% | Sinorhizobium meliloti | |
Fe2+ | 2 mM, specific activity 2.03, relative activity 303% | Sinorhizobium meliloti | |
Mn2+ | 2 mM, specific activity 3.24, relative activity 484% | Sinorhizobium meliloti | |
Ni2+ | 2 mM, specific activity 9.79, relative activity 1461% | Sinorhizobium meliloti |
Application | Comment | Organism |
---|---|---|
biotechnology | production of optically pure L-amino acids by an enzymatic method named hydantoinase process | Sinorhizobium meliloti |
Cloned (Comment) | Organism |
---|---|
the L-N-carbamoylase gene is amplified by PCR and cloned into the pBSK vector for expression of the protein in Escherichia coli BL21(DE3) cells | Sinorhizobium meliloti |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
5,5'-dithiobis-(2-nitrobenzoic acid) | specific activity 0.39, relative activity 58% | Sinorhizobium meliloti | |
beta-mercaptoethanol | specific activity 0.44, relative activity 66% | Sinorhizobium meliloti | |
Cu2+ | 2 mM, specific activity 0.2, relative activity 30% | Sinorhizobium meliloti | |
EDTA | 10 mM, specific activity 0, relative activity 0% | Sinorhizobium meliloti | |
Fe3+ | 2 mM, specific activity 0.44, relative activity 66% | Sinorhizobium meliloti | |
Hg2+ | 2 mM, specific activity 0, relative activity 0% | Sinorhizobium meliloti | |
K+ | 2 mM, specific activity 0.56, relative activity 84% | Sinorhizobium meliloti | |
Na+ | 2 mM, specific activity 0.57, relative activity 85% | Sinorhizobium meliloti | |
Pb2+ | 2 mM, specific activity 0.39, relative activity 58% | Sinorhizobium meliloti | |
Zn2+ | 2 mM, specific activity 0.29, relative activity 43% | Sinorhizobium meliloti |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.65 | - |
N-carbamoyl-L-tryptophan | determined at 40°C for 20 min at pH 8.0 after preincubation with 2 mM Ni2+ | Sinorhizobium meliloti | |
0.69 | - |
N-carbamoyl-L-methionine | determined at 40°C for 20 min at pH 8.0 after preincubation with 2 mM Ni2+ | Sinorhizobium meliloti | |
0.94 | - |
N-carbamoyl-L-alanine | determined at 40°C for 20 min at pH 8.0 after preincubation with 2 mM Ni2+ | Sinorhizobium meliloti | |
2.61 | - |
N-carbamoyl-L-phenylalanine | determined at 40°C for 20 min at pH 8.0 after preincubation with 2 mM Ni2+ | Sinorhizobium meliloti | |
4.8 | - |
N-carbamoyl-L-tyrosine | determined at 40°C for 20 min at pH 8.0 after preincubation with 2 mM Ni2+ | Sinorhizobium meliloti | |
4.91 | - |
N-carbamoyl-L-cysteine | determined at 40°C for 20 min at pH 8.0 after preincubation with 2 mM Ni2+ | Sinorhizobium meliloti | |
5.5 | - |
N-acetyl-L-methionine | determined at 40°C for 20 min at pH 8.0 after preincubation with 2 mM Ni2+ | Sinorhizobium meliloti | |
12.9 | - |
N-formyl-L-methionine | determined at 40°C for 20 min at pH 8.0 after preincubation with 2 mM Ni2+ | Sinorhizobium meliloti | |
34.47 | - |
N-carbamoyl-L-valine | determined at 40°C for 20 min at pH 8.0 after preincubation with 2 mM Ni2+ | Sinorhizobium meliloti | |
51.23 | - |
N-carbamoyl-L-glutamic acid | determined at 40°C for 20 min at pH 8.0 after preincubation with 2 mM Ni2+ | Sinorhizobium meliloti |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
42000 | - |
SDS-PAGE | Sinorhizobium meliloti |
44860 | - |
molecular mass deduced from the amino acid sequence | Sinorhizobium meliloti |
90000 | - |
gel filtration | Sinorhizobium meliloti |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sinorhizobium meliloti | - |
- |
- |
Purification (Comment) | Organism |
---|---|
a one-step purification procedure of the recombinant L-N-carbamoylase fused to the HIS6-tag is employed using immobilized cobalt affinity chromatography | Sinorhizobium meliloti |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.66 | - |
N-carbamoyl-L-methionine | Sinorhizobium meliloti |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
N-acetyl-L-methionine + H2O | - |
Sinorhizobium meliloti | L-methionine + acetate | - |
? | |
N-carbamoyl-L-alanine + H2O | - |
Sinorhizobium meliloti | L-alanine + CO2 + NH3 | - |
? | |
N-carbamoyl-L-cysteine + H2O | - |
Sinorhizobium meliloti | L-cysteine + CO2 + NH3 | - |
? | |
N-carbamoyl-L-glutamic acid + H2O | - |
Sinorhizobium meliloti | L-glutamic acid + CO2 + NH3 | - |
? | |
N-carbamoyl-L-methionine + H2O | activity assay | Sinorhizobium meliloti | L-methionine + CO2 + NH3 | - |
? | |
N-carbamoyl-L-phenylalanine + H2O | - |
Sinorhizobium meliloti | L-phenylalanine + CO2 + NH3 | - |
? | |
N-carbamoyl-L-tryptophan + H2O | - |
Sinorhizobium meliloti | L-tryptophan + CO2 + NH3 | - |
? | |
N-carbamoyl-L-tyrosine + H2O | - |
Sinorhizobium meliloti | L-tyrosine + CO2 + NH3 | - |
? | |
N-carbamoyl-L-valine + H2O | - |
Sinorhizobium meliloti | L-valine + CO2 + NH3 | - |
? | |
N-formyl-L-methionine + H2O | - |
Sinorhizobium meliloti | L-methionine + HCOOH | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | - |
Sinorhizobium meliloti |
Synonyms | Comment | Organism |
---|---|---|
L-N-carbamoylase | - |
Sinorhizobium meliloti |
N-carbamoyl-L-amino-acid hydrolase | - |
Sinorhizobium meliloti |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
activity assay | Sinorhizobium meliloti |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 70 | - |
Sinorhizobium meliloti |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
activity assay | Sinorhizobium meliloti |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 11 | - |
Sinorhizobium meliloti |