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3.5.1.87: N-carbamoyl-L-amino-acid hydrolase

This is an abbreviated version!
For detailed information about N-carbamoyl-L-amino-acid hydrolase, go to the full flat file.

Word Map on EC 3.5.1.87

Reaction

an N-carbamoyl-L-2-amino acid (a 2-ureido carboxylate)
+
H2O
=
an L-2-amino acid
 +
NH3
 +
CO2

Synonyms

ADS79_04835, AtcC, BsLcar, carbamoylated amino acid carbamoylase, hyuC, immobilized L-N-carbamoylase, L-carbamoylase, L-methionine-N-carbamoylase, L-N-carbamoylamino acid aminohydrolase, L-N-carbamoylase, L-NCC amidohydrolase, Lnc, LNCA, N-carbamoyl-amino-acid amidohydrolase, N-carbamoyl-L-alpha-amino acid amidohydrolase, N-carbamoyl-L-amino acid amidohydrolase, N-carbamoyl-L-amino-acid amidohydrolase, N-carbamoyl-L-amino-acid hydrolase, N-carbamoyl-L-cysteine amidohydrolase, N-carbamoyl-L-cysteine-acid amidohydrolase, N-carbamyl-L-amino acid amidohydrolase, NCC amidohydrolase, SmLcar

ECTree

     3 Hydrolases
         3.5 Acting on carbon-nitrogen bonds, other than peptide bonds
             3.5.1 In linear amides
                3.5.1.87 N-carbamoyl-L-amino-acid hydrolase

Purification

Purification on EC 3.5.1.87 - N-carbamoyl-L-amino-acid hydrolase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
a one-step purification procedure of the recombinant L-N-carbamoylase fused to the HIS6-tag is employed using immobilized cobalt affinity chromatography
-
ammonium sulfate, phenyl-Sepharose CL-4B, partially purified
-
ammonium sulfate, phenyl-Sepharose, DEAE-cellulose, phenyl-Sepharose, Sephadex G-100
-
anion-exchange chromatography, hydrophobic interaction chromatography hydroxyapatite chromatography used for purification of native expressed protein
-
DEAE-Sephacel, ammonium sulfate, phenyl-Sepharose CL-4B, Mono Q, Superose 12 HR
-
native enzyme 60fold to homogeneity by heat treatment at 70°C for 2 min, anion exchange chromatography of the supernatant, hydrophobic interaction chromatography, ultrafiltration, and gel filtration
-
one-step purification procedure of SmLcar mutants fused to the His6 tag employing cobalt affinity chromatography
-
purified 58fold to homogeneity with a yield of 16.1% by three steps of column chromatography
-
purified to over 95% purity
-
purity of 95%, purified enzyme is used for analytical analysis and enzyme immobilization
-
recombinant enzyme by cobalt affinity chromatography, ultrafiltration and dialysis
recombinant enzyme from Escherichia coli
recombinant His-tagged enzyme from Escherichia coli by nickel affinity chromatography and dialysis
recombinant His6-tagged enzyme, metal affinity chromatography
-
streamline DEAE, Mono Q
-