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Literature summary for 3.5.1.87 extracted from

  • Wilms, B.; Wiese, A.; Syldatk, C.; Mattes, R.; Altenbuchner, J.; Pietzsch, M.
    Cloning, nucleotide sequence and expression of a new L-N-carbamoylase gene from Arthrobacter aurescens DSM 3747 in E. coli (1999), J. Biotechnol., 68, 101-113.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Paenarthrobacter aurescens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
44000
-
2 * 44000, SDS-PAGE Paenarthrobacter aurescens
93000
-
gel filtration Paenarthrobacter aurescens

Organism

Organism UniProt Comment Textmining
Paenarthrobacter aurescens
-
strain DSM 3747
-

Purification (Commentary)

Purification (Comment) Organism
streamline DEAE, Mono Q Paenarthrobacter aurescens

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
5
-
-
Paenarthrobacter aurescens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N-carbamoyl-DL-phenylalanine + H2O enzyme seems to be selective for aromatic amino acids Paenarthrobacter aurescens L-phenylalanine + CO2 + NH3
-
?
N-carbamoyl-L-methionine + H2O L-stereospecific enzyme Paenarthrobacter aurescens L-methionine + CO2 + NH3
-
?
N-carbamoyl-L-thienylalanine + H2O
-
Paenarthrobacter aurescens L-thienylalanine + CO2 + NH3
-
?
N-carbamoyl-L-tryptophan + H2O
-
Paenarthrobacter aurescens L-tryptophan + CO2 + NH3
-
?
N-carbamoyl-L-tyrosine + H2O
-
Paenarthrobacter aurescens L-tyrosine + CO2 + NH3
-
?
N-formyl-DL-tryptophan + H2O
-
Paenarthrobacter aurescens L-tryptophan + CO2
-
?

Subunits

Subunits Comment Organism
dimer 2 * 44000, SDS-PAGE Paenarthrobacter aurescens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
Paenarthrobacter aurescens

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
25 55 no loss of activity after 6 d at 37°C, 50% loss of activity after 2 h at 50°C Paenarthrobacter aurescens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.9
-
N-carbamoyl-L-methionine
-
Paenarthrobacter aurescens
5
-
N-formyl-DL-tryptophan
-
Paenarthrobacter aurescens
5.1
-
N-carbamoyl-L-tryptophan
-
Paenarthrobacter aurescens
6.5
-
N-carbamoyl-L-tyrosine
-
Paenarthrobacter aurescens
16.1
-
N-carbamoyl-L-thienylalanine
-
Paenarthrobacter aurescens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8 9
-
Paenarthrobacter aurescens

pH Range

pH Minimum pH Maximum Comment Organism
7 11 enzyme activity significantly decreases below, enzyme is highly active up to pH 11.0 Paenarthrobacter aurescens