Literature summary for 3.5.1.87 extracted from

Martinez-Rodriguez, S.; Andujar-Sanchez, M.; Clemente Jimenez, J.M.; Jara-Perez, V.; Rodriguez-Vico, F.; Las Heras-Vazquez, F.J.
Thermodynamic and mutational studies of l-N-carbamoylase from Sinorhizobium meliloti CECT 4114 catalytic centre (2006), Biochimie, 88, 837-847.

Search for more literature for 3.5.1.87

Cloned(Commentary)

Cloned (Comment)	Organism
L-N-carbamoylase gene of Sinorhizobium meliloti was cloned into the pBluescipt II SK (+) vector for expression in Escherichia coli	Sinorhizobium meliloti

Protein Variants

Protein Variants	Comment	Organism
E132D	SmLcar mutant	Sinorhizobium meliloti
E132H	SmLcar mutant	Sinorhizobium meliloti
E132Q	SmLcar mutant	Sinorhizobium meliloti
H230A	SmLcar mutant	Sinorhizobium meliloti
N279A	SmLcar mutant	Sinorhizobium meliloti
R292I	SmLcar mutant	Sinorhizobium meliloti
R292M	SmLcar mutant	Sinorhizobium meliloti

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.2	-	N-carbamoyl-L-methionine	SmLcar mutant E132D	Sinorhizobium meliloti
0.2	-	N-carbamoyl-L-methionine	SmLcar mutant E132Q	Sinorhizobium meliloti
0.6	-	N-carbamoyl-L-methionine	SmLcar mutant E132H	Sinorhizobium meliloti
0.7	-	N-carbamoyl-L-methionine	SmLcar wild type	Sinorhizobium meliloti
1	-	N-carbamoyl-L-methionine	SmLcar mutant H230A	Sinorhizobium meliloti
2.8	-	N-carbamoyl-L-methionine	SmLcar mutant N279A	Sinorhizobium meliloti

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Co2+	-	Sinorhizobium meliloti
Mn2+	-	Sinorhizobium meliloti
Ni2+	-	Sinorhizobium meliloti

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
90000	-	gel filtration	Sinorhizobium meliloti

Organism

Organism	UniProt	Comment	Textmining
Sinorhizobium meliloti	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
one-step purification procedure of SmLcar mutants fused to the His6 tag employing cobalt affinity chromatography	Sinorhizobium meliloti

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
N-carbamoyl-L-methionine + H2O	kinetic assay	Sinorhizobium meliloti	L-methionine + CO2 + NH3	-	?

Synonyms

Synonyms	Comment	Organism
L-N-carbamoylase	-	Sinorhizobium meliloti
N-carbamoyl-L-amino-acid hydrolase	-	Sinorhizobium meliloti
SmLcar	-	Sinorhizobium meliloti

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
40	-	kinetic assay	Sinorhizobium meliloti

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
8	-	kinetic assay	Sinorhizobium meliloti

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Release 2023.1 (January 2023)