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Literature summary for 3.5.1.87 extracted from

  • Martinez-Rodriguez, S.; Garcia-Pino, A.; Las Heras-Vazquez, F.J.; Clemente-Jimenez, J.M.; Rodriguez-Vico, F.; Loris, R.; Garcia-Ruiz, J.M.; Gavira, J.A.
    Crystallization and preliminary crystallographic studies of the recombinant L-N-carbamoylase from Geobacillus stearothermophilus CECT43 (2008), Acta Crystallogr. Sect. F, 64, 1135-1138.
    View publication on PubMedView publication on EuropePMC
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Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21 Geobacillus stearothermophilus

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging-drop vapour diffusion. X-ray data are collected to a resolution of 2.75 A. The crystals belong to space group P21212, with unit-cell parameters a = 103.2, b = 211.7, c = 43.1 A and two subunits in the asymmetric unit Geobacillus stearothermophilus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
44000
-
 SDS-PAGE, very similar to that deduced from its amino acid sequence (about 45 kDa) Geobacillus stearothermophilus
45000
-
 2 * 45000, SDS-PAGE Geobacillus stearothermophilus
90000
-
 gel filtration, SEC–HPLC of purified BsLcar Geobacillus stearothermophilus

Organism

Organism UniProt Comment Textmining
Geobacillus stearothermophilus
-
 CECT43
-
Geobacillus stearothermophilus CECT43
-
 CECT43
-

Purification (Commentary)

Purification (Comment) Organism
purified to over 95% purity Geobacillus stearothermophilus

Subunits

Subunits Comment Organism
dimer 2 * 45000, SDS-PAGE Geobacillus stearothermophilus

Synonyms

Synonyms Comment Organism
BsLcar
-
 Geobacillus stearothermophilus
L-N-carbamoylase
-
 Geobacillus stearothermophilus
N-carbamoyl-L-amino-acid amidohydrolase
-
 Geobacillus stearothermophilus