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Literature summary for 3.5.1.87 extracted from

  • Ishikawa, T.; Watabe, K.; Mukohara, Y.; Nakamura, H.
    N-carbamyl-L-amino acid amidohydrolase of Pseudomonas sp. strain NS671: purification and some properties of the enzyme expressed in Escherichia coli (1996), Biosci. Biotechnol. Biochem., 60, 612-615.
    View publication on PubMed
Search for more literature for 3.5.1.87

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Pseudomonas sp.

Inhibitors

Inhibitors Comment Organism Structure
ATP 0.5 mM, approximately 50% inhibition, 1.5 mM, approximately 85% inhibition Pseudomonas sp.

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+
-
 Pseudomonas sp.
Mn2+
-
 Pseudomonas sp.
Ni2+
-
 Pseudomonas sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
45000
-
 2 * 45000, recombinant enzyme expressed in Escherichia coli, SDS-PAGE Pseudomonas sp.
109000
-
 recombinant enzyme expressed in E. coli, gel filtration Pseudomonas sp.

Organism

Organism UniProt Comment Textmining
Pseudomonas sp.
-
-
-
Pseudomonas sp. NS671
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate, phenyl-Sepharose, DEAE-cellulose, phenyl-Sepharose, Sephadex G-100 Pseudomonas sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
21.5
-
 at pH 7.5 and 42°C Pseudomonas sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N-carbamoyl-DL-alanine + H2O
-
 Pseudomonas sp. L-alanine + CO2 + NH3
-
 ?
N-carbamoyl-DL-alanine + H2O
-
 Pseudomonas sp. NS671 L-alanine + CO2 + NH3
-
 ?
N-carbamoyl-DL-methionine + H2O
-
 Pseudomonas sp. L-methionine + CO2 + NH3
-
 ?
N-carbamoyl-DL-phenylalanine + H2O
-
 Pseudomonas sp. L-phenylalanine + CO2 + NH3
-
 ?
N-carbamoyl-DL-valine + H2O
-
 Pseudomonas sp. L-valine + CO2 + NH3
-
 ?
N-carbamoyl-DL-valine + H2O
-
 Pseudomonas sp. NS671 L-valine + CO2 + NH3
-
 ?
N-carbamoyl-L-isoleucine + H2O
-
 Pseudomonas sp. L-isoleucine + CO2 + NH3
-
 ?
N-carbamoyl-L-isoleucine + H2O
-
 Pseudomonas sp. NS671 L-isoleucine + CO2 + NH3
-
 ?
N-carbamoyl-L-leucine + H2O
-
 Pseudomonas sp. L-leucine + CO2 + NH3
-
 ?
N-carbamoyl-L-leucine + H2O
-
 Pseudomonas sp. NS671 L-leucine + CO2 + NH3
-
 ?
N-carbamoyl-L-methionine + H2O L-stereospecific enzyme Pseudomonas sp. L-methionine + CO2 + NH3
-
 ?
N-carbamoyl-L-methionine + H2O L-stereospecific enzyme Pseudomonas sp. NS671 L-methionine + CO2 + NH3
-
 ?
N-carbamoyl-L-tyrosine + H2O
-
 Pseudomonas sp. L-tyrosine + CO2 + NH3
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 45000, recombinant enzyme expressed in Escherichia coli, SDS-PAGE Pseudomonas sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
 Pseudomonas sp.

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
25 50
-
 Pseudomonas sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
25
-
 activity is lost after 60 min at 25°C Pseudomonas sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
 Pseudomonas sp.

pH Range

pH Minimum pH Maximum Comment Organism
5.5 9
-
 Pseudomonas sp.