3.4.24.B14: neprilysin-2

This is an abbreviated version!
For detailed information about neprilysin-2, go to the full flat file.

Reaction

proteolytic degradation of proteins =

Synonyms

M13.008, membrane metallo-endopeptidase-like protein, membrane-bound metalloendopeptidase-like enzyme 1, membrane-bound metalloendopeptidase-like enzyme 2, MMEL, MMEL1, MMEL2, More, NEP-like endopeptidase, NEP2, NEP2(s), NEPII, NEPLP, neprilysin 2, neprilysin-2, neprilysin-like protein, NL-1, Nl1, NL1 protein, SEP, soluble-secreted endopeptidase

ECTree

     3 Hydrolases

         3.4 Acting on peptide bonds (peptidases)

             3.4.24 Metalloendopeptidases

                3.4.24.B14 neprilysin-2

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Results	in table
3	AA Sequence
1	Application
2	CAS Registry Number
9	Cloned(Commentary)
18	Disease/ Diagnostics
2	Expression
15	General Information
6	IC50 Value
57	Inhibitors
35	Ki Value [mM]
12	KM Value [mM]
20	Localization
3	Metals/Ions
4	Molecular Weight [Da]
7	Natural Substrates/ Products (Substrates)
19	Organism
2	pH Optimum
1	pH Stability
7	Posttranslational Modification
4	Protein Variants
2	Purification (Commentary)
1	Reaction
2	Reaction Type
19	Reference
33	Source Tissue
65	Substrates and Products (Substrate)
1	Subunits
44	Synonyms
1	Temperature Optimum [°C]
30	Turnover Number [1/s]

KM Value

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KM Value on EC 3.4.24.B14 - neprilysin-2

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KM VALUE [mM]

SUBSTRATE

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

IMAGE

0.023 - 0.035

3-methoxysuccinyl-Gly-Leu-Phe-Met-7-amido-4-methylcoumarin

2 entries

0.04 - 1

succinyl-Ala-Ala-Phe-4-methylcoumarin 7-amide

6 entries

0.027 - 0.05

succinyl-Ala-Ala-Phe-7-amido-4-methylcoumarin

2 entries

0.04 - 0.1

succinyl-Ala-Ala-Val-7-amido-4-methylcoumarin

2 entries

0.023

3-methoxysuccinyl-Gly-Leu-Phe-Met-7-amido-4-methylcoumarin

Rattus norvegicus

P0C1T0

37°C, membrane-bound form of NEP2

690798

0.035

3-methoxysuccinyl-Gly-Leu-Phe-Met-7-amido-4-methylcoumarin

Rattus norvegicus

P0C1T0

37°C, secreted soluble form of NEP2

690798

0.04

succinyl-Ala-Ala-Phe-4-methylcoumarin 7-amide

Rattus norvegicus

-

37°C, pH 7.2, mutant enzyme L739G

669280

0.044

succinyl-Ala-Ala-Phe-4-methylcoumarin 7-amide

Rattus norvegicus

-

37°C, pH 7.2, mutant enzyme L739G/S133G

669280

0.045

succinyl-Ala-Ala-Phe-4-methylcoumarin 7-amide

Rattus norvegicus

-

37°C, pH 7.2, mutant enzyme R131M

669280

0.062

succinyl-Ala-Ala-Phe-4-methylcoumarin 7-amide

Rattus norvegicus

-

37°C, pH 7.2. wild-type enzyme

669280

0.185

succinyl-Ala-Ala-Phe-4-methylcoumarin 7-amide

Rattus norvegicus

-

37°C, pH 7.2, mutant enzyme S133G

669280

1

succinyl-Ala-Ala-Phe-4-methylcoumarin 7-amide

Rattus norvegicus

-

37°C, pH 7.2, mutant enzyme N567G

669280

0.027

succinyl-Ala-Ala-Phe-7-amido-4-methylcoumarin

Rattus norvegicus

P0C1T0

37°C, membrane-bound form of NEP2

690798

0.05

succinyl-Ala-Ala-Phe-7-amido-4-methylcoumarin

Rattus norvegicus

P0C1T0

37°C, secreted soluble form of NEP2

690798

0.04

succinyl-Ala-Ala-Val-7-amido-4-methylcoumarin

Rattus norvegicus

P0C1T0

37°C, membrane-bound form of NEP2

690798

0.1

succinyl-Ala-Ala-Val-7-amido-4-methylcoumarin

Rattus norvegicus

P0C1T0

37°C, secreted soluble form of NEP2

690798