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3.4.24.34: neutrophil collagenase

This is an abbreviated version!
For detailed information about neutrophil collagenase, go to the full flat file.

Word Map on EC 3.4.24.34

Reaction

Cleavage of interstitial collagens in the triple helical domain. Unlike EC 3.4.24.7, interstitial collagenase, this enzyme cleaves type III collagen additional information slowly than type I =

Synonyms

collagenase-2, collagenases-8, EC 3.4.24.7, HNC, human neutrophil collagenase, matrix metalloproteinase, Matrix metalloproteinase 8, Matrix metalloproteinase-8, metalloproteinase-8, MetMMP-8, MMP-7, MMP-8, MMP8, neutrophil collagenase, neutrophil collagenase MMP-8, neutrophil interstitial collagenase, PheMMP-8, PMNL collagenase, PMNL-CL, polymorphonuclear leukocyte collagenase, whMMP-8

ECTree

     3 Hydrolases
         3.4 Acting on peptide bonds (peptidases)
             3.4.24 Metalloendopeptidases
                3.4.24.34 neutrophil collagenase

Crystallization

Crystallization on EC 3.4.24.34 - neutrophil collagenase

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CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
crystal structure of the catalytic domain in non-covalent complex with the hydroxamate inhibitor BB-1909, space group P2(1)2(1)2(1), cell constants a : 4.47 nm, b : 8.08 nm, c : 10.81 nm
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crystal structures of MMP-8 have been solved, PDB IDs 3DNG, 3DPE, 1ZVX
crystallized using hanging drop method, space group P2(1)2(1)2(1), cell dimesions a : 33.1 A, b : 68.9 A, c : 70.5 A
in complex with a primed and an unprimed-side inhibitor, hanging-drop vapor diffusion, space group P2(1)2(1)2(1), cell dimensions a : 32.98 A, b : 68.67 A, c : 70.49 A
in complex with inhibitor HONH-iBM-L-Ala-Gly-NH2, vapor-diffusion technique, hanging drop
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MMP-8 in complex with inhibitors (S)- and (R)-alpha-arylsulfonylamino phosphonate, hanging drop vapor diffusion method at 18°C, mixing of 0.0015 ml of protein solution containing 6 mg/mL protein in 5 mM CaCl2, 100 mM NaCl, 0.5 mM ZnCl2, 3 mM MES-NaOH, 0.02% NaN3, pH 6.0, with 0.001 ml of inhibitor solution containing 1 mM inhibitor in 0.2 M MES-NaOH, 20% MeOH, pH 6.0, and 0.005 ml of PEG solution containing 10% m/v PEG 6000, 0.2 M MES-NaOH, 0.02% NaN3, pH 6.0, droplets are concentrated against a reservoir buffer containing 1.6 M sodium phosphate buffer, 0.02% NaN3, pH 6.0, X-ray diffraction structure determination and anaylsis at 1.56-1.94 A resolution
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