3.4.23.16: HIV-1 retropepsin
This is an abbreviated version!
For detailed information about HIV-1 retropepsin, go to the full flat file.
Word Map on EC 3.4.23.16
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3.4.23.16
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antiretroviral
-
ritonavir
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transcriptase
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saquinavir
-
indinavir
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nelfinavir
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hiv-infected
-
lopinavir
-
polyproteins
-
flap
-
darunavir
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amprenavir
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drug-resistant
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anti-hiv
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atazanavir
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virological
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haart
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gag-pol
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peptidomimetic
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virion
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p-glycoprotein
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lipodystrophy
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non-nucleoside
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integrase
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cross-resistance
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isostere
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nonpeptidic
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zidovudine
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nevirapine
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virus-1
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tipranavir
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cyp3a4
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nnrti
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drug-drug
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treatment-experienced
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lamivudine
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didanosine
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ritonavir-boosted
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autoprocessing
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efavirenz
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protease-inhibitor
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stavudine
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treatment-naive
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analysis
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raltegravir
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zalcitabine
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plasmepsins
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antiretroviral-naive
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pi-based
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pharmacology
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drug development
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medicine
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hiv-rna
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lipoatrophy
- 3.4.23.16
-
antiretroviral
- ritonavir
- transcriptase
- saquinavir
- indinavir
- nelfinavir
-
hiv-infected
- lopinavir
- polyproteins
- flap
- darunavir
- amprenavir
-
drug-resistant
-
anti-hiv
- atazanavir
-
virological
-
haart
- gag-pol
-
peptidomimetic
- virion
- p-glycoprotein
- lipodystrophy
-
non-nucleoside
-
integrase
-
cross-resistance
- isostere
-
nonpeptidic
- zidovudine
- nevirapine
- virus-1
- tipranavir
- cyp3a4
-
nnrti
-
drug-drug
-
treatment-experienced
- lamivudine
- didanosine
-
ritonavir-boosted
-
autoprocessing
- efavirenz
-
protease-inhibitor
- stavudine
-
treatment-naive
- analysis
- raltegravir
- zalcitabine
-
plasmepsins
-
antiretroviral-naive
-
pi-based
- pharmacology
- drug development
- medicine
-
hiv-rna
-
lipoatrophy
Reaction
specific for a P1 residue that is hydrophobic, and P1' variable, but often Pro =
Synonyms
CRF01_AE protease, Gag protease, HIV aspartyl protease, HIV PR, HIV protease, HIV-1 aspartyl protease, HIV-1 PR, HIV-1 protease, HIV-1 proteinase, HIV-1PR, HIV-2 protease, HIVPR, human immunodeficiency virus 1 protease, human immunodeficiency virus 1 retropepsin, human immunodeficiency virus protease, human immunodeficiency virus type 1 protease, human immunodeficiency virus type I protease, More, PR, PR1, PR2, retropepsin, retroproteinase
ECTree
3.4.23.16 HIV-1 retropepsinAdvanced search results
results (
results (KM Value
KM Value on EC 3.4.23.16 - HIV-1 retropepsin
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KM VALUE [mM] 
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
IMAGE
0.025
Arg-Glu(EDANS)-Ser-Gln-Asn-Tyr-Pro-Ile-Val-Gln-Lys(DABCYL)-Arg
-
-
0.0098
His-Lys-Ala-Arg-Val-Leu-(4-nitro)Phe-Glu-Ala-Nle-Ser-amide
-
-
2
VSQNYPIV
-
pH 5.5, 30°C, wild-type enzyme and mutant enzyme Q7K/L33I/L63I
0.00069
2-aminobenzoyl-TI-Nle-Phe(NO2)-ER
-
pH 5.2, 2.0 M NaCl
0.00084
2-aminobenzoyl-TI-Nle-Phe(NO2)-ER
-
pH 4.5, 0.1 M NaCl
0.00598
2-aminobenzoyl-TI-Nle-Phe(NO2)-QR
-
pH 5.0, 2.0 M NaCl
0.00873
2-aminobenzoyl-TI-Nle-Phe(NO2)-QR
-
pH 4.9, 1.0 M NaCl
0.0097
Abz-Ala-Arg-Val-Nle-(p-nitro)Phe-Phe-Glu-Ala-Nle-NH2
-
wild-type, pH 5.5
0.0513
Abz-Ala-Arg-Val-Nle-(p-nitro)Phe-Phe-Glu-Ala-Nle-NH2
-
mutant I84V, pH 5.5
0.117
Abz-Ala-Arg-Val-Nle-(p-nitro)Phe-Phe-Glu-Ala-Nle-NH2
-
mutant I50V, pH 5.5
0.0052
Abz-Arg-Val-Nle-(p-nitro)Phe-Phe-Glu-Ala-Nle-NH2
-
wild-type, pH 5.5
0.0277
Abz-Arg-Val-Nle-(p-nitro)Phe-Phe-Glu-Ala-Nle-NH2
-
I84V, pH 5.5
0.0549
Abz-Arg-Val-Nle-(p-nitro)Phe-Phe-Glu-Ala-Nle-NH2
-
I50V, pH 5.5
0.0227
Abz-Lys-Ala-Arg-Val-Nle-(p-nitro)Phe-Phe-Glu-Ala-Nle-NH2
-
wild-type, pH 5.5
0.072
Abz-Lys-Ala-Arg-Val-Nle-(p-nitro)Phe-Phe-Glu-Ala-Nle-NH2
-
mutant I84V, pH 5.5
0.158
Abz-Lys-Ala-Arg-Val-Nle-(p-nitro)Phe-Phe-Glu-Ala-Nle-NH2
-
mutant I50V, pH 5.5
0.0146
Abz-Thr-Ile-Nle-(p-nitro)Phe-Gln-Arg-NH2
-
wild-type, pH 5.5
0.0706
Abz-Thr-Ile-Nle-(p-nitro)Phe-Gln-Arg-NH2
-
mutant I84V, pH 5.5
0.139
Abz-Thr-Ile-Nle-(p-nitro)Phe-Gln-Arg-NH2
-
mutant I50V, pH 5.5
0.03
Abz-Thr-Ile-Nle-Phe(NO2)-Gln-Arg
-
wild-type, pH 5.6, 26°C
0.041
Abz-Thr-Ile-Nle-Phe(NO2)-Gln-Arg
-
mutant I54M, pH 5.6, 26°C
0.043
Abz-Thr-Ile-Nle-Phe(NO2)-Gln-Arg
-
mutant I54V, pH 5.6, 26°C
0.091
Abz-Thr-Ile-Nle-Phe(NO2)-Gln-Arg
-
mutant G48V, pH 5.6, 26°C
0.109
Abz-Thr-Ile-Nle-Phe(NO2)-Gln-Arg
-
mutant I50V, pH 5.6, 26°C
0.032
AETFYVDGAA
pH 5.6, 37°C, autolysis resistant enzyme form PR with the mutations Q7K/L33I/L63I/C67A/C95A
0.041
AETFYVDGAA
pH 5.6, 37°C, autolysis reistant and truncated enzyme form PR-(5-99) with mutation Q7K/L33I/L63I/C67A/C95A
0.044
CTLNFPISP
pH 5.6, 37°C, autolysis resistant enzyme form PR with the mutations Q7K/L33I/L63I/C67A/C95A
0.087
CTLNFPISP
pH 5.6, 37°C, autolysis reistant and truncated enzyme form PR-(5-99) with mutation Q7K/L33I/L63I/C67A/C95A
0.07
IPFAAAQQRK
mutant enzyme V32I/I47V/V82I, in 0.5 M potassium phosphate buffer, pH 5.6, containing 10% (v/v) glycerol, 2 mM EDTA, 10 mM dithiothreitol, 4 M NaCl, at 37°C
0.28
IPFAAAQQRK
wild type enzyme, in 0.5 M potassium phosphate buffer, pH 5.6, containing 10% (v/v) glycerol, 2 mM EDTA, 10 mM dithiothreitol, 4 M NaCl, at 37°C
0.016
IRKILFLDG
pH 5.6, 37°C, autolysis resistant enzyme form PR with the mutations Q7K/L33I/L63I/C67A/C95A
0.046
IRKILFLDG
pH 5.6, 37°C, autolysis reistant and truncated enzyme form PR-(5-99) with mutation Q7K/L33I/L63I/C67A/C95A
0.09
KARLMAEALK
mutant enzyme V32I/I47V/V82I, in 0.5 M potassium phosphate buffer, pH 5.6, containing 10% (v/v) glycerol, 2 mM EDTA, 10 mM dithiothreitol, 4 M NaCl, at 37°C
0.13
KARLMAEALK
wild type enzyme, in 0.5 M potassium phosphate buffer, pH 5.6, containing 10% (v/v) glycerol, 2 mM EDTA, 10 mM dithiothreitol, 4 M NaCl, at 37°C
0.075
KARV-Nle-Phe(NO2)-EA-Nle
-
pH 5.0, mutant enzyme Q7K/L33I/L63I/C67A/C95A
0.133
KARV-Nle-Phe(NO2)-EA-Nle-NH2
-
pH 5.0, 25°C, wild-type enzyme
0.177
KARV-Nle-Phe(NO2)-EA-Nle-NH2
-
pH 5.0, 25°C, mutant enzyme Q7K/L33I/L63I/C67A/C95A
0.225
KARV-Nle-Phe(NO2)-EA-Nle-NH2
-
pH 5.0, 25°C, mutant enzyme Q7K/R8Q/L33I/L63I/C67A/C95A
0.231
KARV-Nle-Phe(NO2)-EA-Nle-NH2
-
pH 5.0, 25°C, mutant enzyme C95A
0.244
KARV-Nle-Phe(NO2)-EA-Nle-NH2
-
pH 5.0, 25°C, mutant enzyme C67A/C95A
0.263
KARV-Nle-Phe(NO2)-EA-Nle-NH2
-
pH 5.0, 25°C, mutant enzyme C67A
0.278
KARV-Nle-Phe(NO2)-EA-Nle-NH2
-
pH 5.0, 25°C, wild-type enzyme
0.66
KARV-Nle-Phe(NO2)-EA-Nle-NH2
-
pH 5.0, 25°C, truncated version PR-(5-99) from mutant enzyme Q7K/L33I/L63I/C67A/C95A
0.021
KARVLAEAMS
pH 5.6, 37°C, autolysis resistant enzyme form PR with the mutations Q7K/L33I/L63I/C67A/C95A
0.192
KARVLAEAMS
pH 5.6, 37°C, autolysis reistant and truncated enzyme form PR-(5-99) with mutation Q7K/L33I/L63I/C67A/C95A
0.044
Lys-Ala-Arg-Val-Nle-4-nitro-Phe-Glu-Ala-Nle-amide
-
pH 5.0, mutant V82A
0.046
Lys-Ala-Arg-Val-Nle-4-nitro-Phe-Glu-Ala-Nle-amide
-
pH 5.0, 25°C, mutant G73S
0.055
Lys-Ala-Arg-Val-Nle-4-nitro-Phe-Glu-Ala-Nle-amide
-
pH 5.0, mutant Q7K/L33I/L63I/C67A/C95A
0.211
Lys-Ala-Arg-Val-Nle-4-nitro-Phe-Glu-Ala-Nle-amide
-
pH 5.0, mutant I84V
0.31
Lys-Ala-Arg-Val-Nle-4-nitro-Phe-Glu-Ala-Nle-amide
-
pH 5.0, 25°C, mutant L24I
0.5
Lys-Ala-Arg-Val-Nle-4-nitro-Phe-Glu-Ala-Nle-amide
-
pH 5.0, 25°C, mutant I50V
0.01
Lys-Ala-Arg-Val-Nle-Phe(4-NO2)-Glu-Ala-Nle-Gly
-
37°C, pH 4.7, HIV-1 subtype B protease mutant L90M
0.016
Lys-Ala-Arg-Val-Nle-Phe(4-NO2)-Glu-Ala-Nle-Gly
-
37°C, pH 4.7, HIV-1 subtype B protease mutant N88D
0.017
Lys-Ala-Arg-Val-Nle-Phe(4-NO2)-Glu-Ala-Nle-Gly
-
37°C, pH 4.7, HIV-1 subtype C protease
0.019
Lys-Ala-Arg-Val-Nle-Phe(4-NO2)-Glu-Ala-Nle-Gly
-
37°C, pH 4.7, HIV-1 subtype B protease D30N/N88D
0.0195
Lys-Ala-Arg-Val-Nle-Phe(4-NO2)-Glu-Ala-Nle-Gly
-
37°C, pH 4.7, HIV-1 subtype B protease
0.022
Lys-Ala-Arg-Val-Nle-Phe(4-NO2)-Glu-Ala-Nle-Gly
-
37°C, pH 4.7, HIV-1 subtype B protease mutant N88D
0.023
Lys-Ala-Arg-Val-Nle-Phe(4-NO2)-Glu-Ala-Nle-Gly
-
37°C, pH 4.7, HIV-1 subtype B protease D30N/L90M
0.023
Lys-Ala-Arg-Val-Nle-Phe(4-NO2)-Glu-Ala-Nle-Gly
-
37°C, pH 4.7, HIV-1 subtype B protease mutant L90M
0.024
Lys-Ala-Arg-Val-Nle-Phe(4-NO2)-Glu-Ala-Nle-Gly
-
37°C, pH 4.7, HIV-1 subtype B protease N88D/L90M
0.03
Lys-Ala-Arg-Val-Nle-Phe(4-NO2)-Glu-Ala-Nle-Gly
-
37°C, pH 4.7, HIV-1 subtype B protease N88D/L90M
0.035
Lys-Ala-Arg-Val-Nle-Phe(4-NO2)-Glu-Ala-Nle-Gly
-
37°C, pH 4.7, HIV-1 subtype B protease mutant 0.061 D30N/N88D
0.0386
Lys-Ala-Arg-Val-Nle-Phe(4-NO2)-Glu-Ala-Nle-Gly
-
37°C, pH 4.7, HIV-1 subtype B protease mutant D30N
0.061
Lys-Ala-Arg-Val-Nle-Phe(4-NO2)-Glu-Ala-Nle-Gly
-
37°C, pH 4.7, HIV-1 subtype B protease mutant 0.03 D30M/L90M
0.064
Lys-Ala-Arg-Val-Nle-Phe(4-NO2)-Glu-Ala-Nle-Gly
-
37°C, pH 4.7, HIV-1 subtype C protease mutant D30N
0.006
Lys-Ala-Arg-Val-Nle-Phe(4-NO2)-Glu-Ala-Nle-NH2
-
37°C, pH 4.7, mutant enzyme N88D
0.011
Lys-Ala-Arg-Val-Nle-Phe(4-NO2)-Glu-Ala-Nle-NH2
-
37°C, pH 4.7, mutant enzyme L90M
0.014
Lys-Ala-Arg-Val-Nle-Phe(4-NO2)-Glu-Ala-Nle-NH2
-
37°C, pH 4.7, mutant enzyme A71V
0.015
Lys-Ala-Arg-Val-Nle-Phe(4-NO2)-Glu-Ala-Nle-NH2
-
37°C, pH 4.7, wild-type enzyme
0.018
Lys-Ala-Arg-Val-Nle-Phe(4-NO2)-Glu-Ala-Nle-NH2
-
37°C, pH 4.7, mutant enzyme D30N
0.018
Lys-Ala-Arg-Val-Nle-Phe(4-NO2)-Glu-Ala-Nle-NH2
-
37°C, pH 4.7, mutant enzyme D30N/N88D
0.024
Lys-Ala-Arg-Val-Nle-Phe(4-NO2)-Glu-Ala-Nle-NH2
-
37°C, pH 4.7, mutant enzyme D30N/A71V
0.027
Lys-Ala-Arg-Val-Nle-Phe(4-NO2)-Glu-Ala-Nle-NH2
-
37°C, pH 4.7, mutant enzyme D30N/L63PA71V/L90M
0.029
Lys-Ala-Arg-Val-Nle-Phe(4-NO2)-Glu-Ala-Nle-NH2
-
37°C, pH 4.7, mutant enzyme D30N/L90M
0.34
RQGTVSFNFPQITL
-
pH 5.0, 37°C, mutant enzyme A28S
0.54
RQGTVSFNFPQITL
-
pH 5.0, 37°C, wild-type enzyme
0.32
VLQNYPIVQ
mutant enzyme V32I/I47V/V82I, in 0.5 M potassium phosphate buffer, pH 5.6, containing 10% (v/v) glycerol, 2 mM EDTA, 10 mM dithiothreitol, 4 M NaCl, at 37°C
0.4
VLQNYPIVQ
wild type enzyme, in 0.5 M potassium phosphate buffer, pH 5.6, containing 10% (v/v) glycerol, 2 mM EDTA, 10 mM dithiothreitol, 4 M NaCl, at 37°C
0.12
VSQLYPIVQ
wild type enzyme, in 0.5 M potassium phosphate buffer, pH 5.6, containing 10% (v/v) glycerol, 2 mM EDTA, 10 mM dithiothreitol, 4 M NaCl, at 37°C
0.38
VSQLYPIVQ
mutant enzyme V32I/I47V/V82I, in 0.5 M potassium phosphate buffer, pH 5.6, containing 10% (v/v) glycerol, 2 mM EDTA, 10 mM dithiothreitol, 4 M NaCl, at 37°C
0.24
VSQVYPIVQ
wild type enzyme, in 0.5 M potassium phosphate buffer, pH 5.6, containing 10% (v/v) glycerol, 2 mM EDTA, 10 mM dithiothreitol, 4 M NaCl, at 37°C
0.35
VSQVYPIVQ
mutant enzyme V32I/I47V/V82I, in 0.5 M potassium phosphate buffer, pH 5.6, containing 10% (v/v) glycerol, 2 mM EDTA, 10 mM dithiothreitol, 4 M NaCl, at 37°C
0.19
VVQNYPIVQ
wild type enzyme, in 0.5 M potassium phosphate buffer, pH 5.6, containing 10% (v/v) glycerol, 2 mM EDTA, 10 mM dithiothreitol, 4 M NaCl, at 37°C
0.23
VVQNYPIVQ
mutant enzyme V32I/I47V/V82I, in 0.5 M potassium phosphate buffer, pH 5.6, containing 10% (v/v) glycerol, 2 mM EDTA, 10 mM dithiothreitol, 4 M NaCl, at 37°C
additional information
additional information
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kinetics, LAI wild-type enzyme
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additional information
additional information
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molecular dynamics technique simulation of pH effecting the enzyme dynamics, effects of Na+ and Cl- ions, overview
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additional information
additional information
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substrate specificity and kinetics of wild-type and mutant enzymes, overview
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