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Literature summary for 3.4.23.16 extracted from

  • Ermolieff, J.; Lin, X.; Tang, J.
    The effect of substrates on the kinetics and the in vivo threshold activity of mutant HIV-1 proteases (1998), Adv. Exp. Med. Biol., 436, 47-51.
    View publication on PubMed
Search for more literature for 3.4.23.16

Protein Variants

Protein Variants Comment Organism
A28S with VSQNYPIVQ the turnover-number of the mutant enzyme is 37% of that of the wild-type enzyme Human immunodeficiency virus 1
G48H with VSQNYPIVQ the turnover-number of the mutant enzyme is 60% of that of the wild-type enzyme, with VSQNYPIVQ the KM-value of the mutant enzyme is 2.2fold higher than the Km-value of the wild-type enzyme Human immunodeficiency virus 1
G48V the kcat/Km ratio for the substrate KARVLAEAMS is 55% of that for the autoproteolysis resistant variant Q7K/L33I/L63I/C67A/C95A Human immunodeficiency virus 1
G48V mutant enzyme with 9.3% of the activity of the wild-type enzyme with VSQNYPIVQ as substrate Human immunodeficiency virus 1
I84V with VSQNYPIVQ the turnover-number of the mutant enzyme is 19% of that of the wild-type enzyme, with VSQNYPIVQ the KM-value of the mutant enzyme is 7.2fold higher than the Km-value of the wild-type enzyme Human immunodeficiency virus 1
L90M with VSQNYPIVQ the turnover-number of the mutant enzyme is 6% of that of the wild-type enzyme, with VSQNYPIVQ the KM-value of the mutant enzyme is 76% of the Km-value of the wild-type enzyme Human immunodeficiency virus 1
V82A with VSQNYPIVQ the turnover-number of the mutant enzyme is 40% of that of the wild-type enzyme, with VSQNYPIVQ the KM-value of the mutant enzyme is 2.8fold higher than the Km-value of the wild-type enzyme Human immunodeficiency virus 1
V82E with VSQNYPIVQ the turnover-number of the mutant enzyme is 60% of that of the wild-type enzyme, with VSQNYPIVQ the KM-value of the mutant enzyme is 6.9fold higher than the Km-value of the wild-type enzyme Human immunodeficiency virus 1

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.34
-
 RQGTVSFNFPQITL pH 5.0, 37°C, mutant enzyme A28S Human immunodeficiency virus 1
0.54
-
 RQGTVSFNFPQITL pH 5.0, 37°C, wild-type enzyme Human immunodeficiency virus 1
1.15
-
 VSQNYPIVQ pH 5.0, 37°C, mutant enzyme L90M Human immunodeficiency virus 1
1.5
-
 VSQNYPIVQ pH 5.0, 37°C, wild-type enzyme Human immunodeficiency virus 1
3.3
-
 VSQNYPIVQ pH 5.0, 37°C, mutant enzyme G48H Human immunodeficiency virus 1
3.8
-
 VSQNYPIVQ pH 5.0, 37°C, mutant enzyme G48V/L90M Human immunodeficiency virus 1
4.2
-
 VSQNYPIVQ pH 5.0, 37°C, mutant enzyme V82A Human immunodeficiency virus 1
6.4
-
 KLRPGNFLQSRP pH 5.0, 37°C, wild-type enzyme Human immunodeficiency virus 1

Organism

Organism UniProt Comment Textmining
Human immunodeficiency virus 1
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
KGRPGNFLQSRP + H2O
-
 Human immunodeficiency virus 1 KGRPGNF + LQSRP
-
 ?
KLRPGNFLQSRP + H2O
-
 Human immunodeficiency virus 1 KLRPGNF + LQSRP
-
 ?
RQGTVSFNFPQITL + H2O
-
 Human immunodeficiency virus 1 RQGTVSFNF + PQITL
-
 ?
VSQNYPIVQ + H2O
-
 Human immunodeficiency virus 1 VSQNY + PIVQ
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.08
-
 VSQNYPIVQ pH 5.0, 37°C, mutant enzyme I84V Human immunodeficiency virus 1
0.172
-
 VSQNYPIVQ pH 5.0, 37°C, mutant enzyme V82E Human immunodeficiency virus 1
2.2
-
 VSQNYPIVQ pH 5.0, 37°C, mutant enzyme G48V/L90M Human immunodeficiency virus 1
3.14
-
 VSQNYPIVQ pH 5.0, 37°C, mutant enzyme L90M Human immunodeficiency virus 1
9.7
-
 VSQNYPIVQ pH 5.0, 37°C, mutant enzyme I82V Human immunodeficiency virus 1
12.7
-
 KLRPGNFLQSRP pH 5.0, 37°C, wild-type enzyme Human immunodeficiency virus 1
19.2
-
 VSQNYPIVQ pH 5.0, 37°C, mutant enzyme A28S Human immunodeficiency virus 1
20.7
-
 VSQNYPIVQ pH 5.0, 37°C, mutant enzyme V82A Human immunodeficiency virus 1
30.9
-
 VSQNYPIVQ pH 5.0, 37°C, mutant enzyme V82E Human immunodeficiency virus 1
31.3
-
 VSQNYPIVQ pH 5.0, 37°C, mutant enzyme G48V Human immunodeficiency virus 1
51.8
-
 VSQNYPIVQ pH 5.0, 37°C, wild-type enzyme Human immunodeficiency virus 1
62.7
-
 RQGTVSFNFPQITL pH 5.0, 37°C, wild-type enzyme Human immunodeficiency virus 1
93.5
-
 VSQNYPIVQ pH 5.0, 37°C, mutant enzyme G48H Human immunodeficiency virus 1