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Literature summary for 3.4.23.16 extracted from

  • Meek, T.D.; Rodriguez, E.J.; Angeles, T.S.
    Use of steady state kinetic methods to elucidate the kinetic and chemical mechanisms of retroviral proteases (1994), Methods Enzymol., 241, 127-156.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
Ac-RASQNY product inhibition with Ac-SQNYPVV as substrate Human immunodeficiency virus 1
Ac-RKIL product inhibition with Ac-RKILFLDG-NH2 as substrate Human immunodeficiency virus 1
Ac-SQNY product inhibition with Ac-RASQNYPVV or Ac-SQNYFLDG-NH2 as substrate Human immunodeficiency virus 1
Phe-Leu-Asp-Gly product inhibition with Ac-RKILFLDG-NH2 as substrate Human immunodeficiency virus 1
Phe-Leu-Asp-Gly-NH2 product inhibition with Ac-SQNYFLDG-NH2 as substrate Human immunodeficiency virus 1

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.012
-
Ac-HGWILAEHGD-NH2 pH 6.0, 37°C Human immunodeficiency virus 1
0.66
-
Ac-SQNYFLDG-NH2 pH 6.0, 37°C Human immunodeficiency virus 1
1.5
-
Ac-SQSYPVV-NH2 pH 6.0, 37°C Human immunodeficiency virus 1
2.1
-
Ac-RKILFLDG-NH2 pH 6.0, 37°C Human immunodeficiency virus 1
3.9
-
Ac-RASQNYPVV-NH2 pH 6.0, 37°C Human immunodeficiency virus 1
5.5
-
Ac-SQNYPVV-NH2 pH 6.0, 37°C Human immunodeficiency virus 1

Metals/Ions

Metals/Ions Comment Organism Structure
additional information the activity of the enzyme is dependent on the ionic strength of the reaction mixture, rather than on the binding of a specific ion to an enzymatic site Human immunodeficiency virus 1

Organism

Organism UniProt Comment Textmining
Human immunodeficiency virus 1
-
-
-

Reaction

Reaction Comment Organism Reaction ID
specific for a P1 residue that is hydrophobic, and P1' variable, but often Pro the kinetic mechanism of HIV-1 protease is random, in which products bind to form both binary and ternary enzyme-product complexes, depending on the substrate used Human immunodeficiency virus 1

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Ac-HGWILAEHGD-NH2 + H2O
-
Human immunodeficiency virus 1 Ac-HGWIL + AEHGD-NH2
-
?
Ac-RASQNYPVV-NH2 + H2O
-
Human immunodeficiency virus 1 Ac-RASQNY + PVV-NH2
-
?
Ac-RKILFLDG-NH2 + H2O
-
Human immunodeficiency virus 1 Ac-RKIL + FLDG-NH2
-
?
Ac-SQNYFLDG-NH2 + H2O
-
Human immunodeficiency virus 1 Ac-SQNY + FLDG-NH2
-
?
Ac-SQNYPVV-NH2 + H2O
-
Human immunodeficiency virus 1 Ac-SQNY + PVV-NH2
-
?
Ac-SQSYPVV-NH2 + H2O
-
Human immunodeficiency virus 1 Ac-SQSY + PVV-NH2
-
?