3.4.22.B20: PfpI endopeptidase
This is an abbreviated version!
For detailed information about PfpI endopeptidase, go to the full flat file.
Reaction
efficient cleavage of Ala-Ala-Phe-/-7-amido-4-methylcoumarinamide. Dominant protease in Pyrococcus furiosus =
Synonyms
C56.001, PfpI, PfpI endopeptidase, PfpI protease, Pyrococcus furiosus protease I
ECTree
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Temperature Stability
Temperature Stability on EC 3.4.22.B20 - PfpI endopeptidase
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90
half life is 45 min, in presence of 0.5 M KHPO4 the half-life is extended to 80 min, activity with Ala-Ala-Phe-7-amido-4-methylcoumarinamide
95
98
95
in presence of 2% SDS and 80 nM dithiothreitol does not dissociate the protein, half-life: 19 min
half life is below 30 min, in presence of 0.8 M KHPO4 the half-life is extended to 110 min, activity with Ala-Ala-Phe-7-amido-4-methylcoumarinamide
98
the enzyme has a 33 h half-life at 98°C after heating in 1% SDS