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Literature summary for 3.4.22.B20 extracted from
- Pyrococcus furiosus protease I (2004), Handbook of Proteolytic Enzymes (Barrett, A. J. ; Rawlings, N. D. ; Woessner, eds. ), 2, 1393-1395.
No PubMed abstract available
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Pyrococcus furiosus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| DFP | - |
Pyrococcus furiosus |  |
| PMSF | - |
Pyrococcus furiosus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 18800 | - |
monomer, calculated from amino acid sequence | Pyrococcus furiosus |
| 59000 | - |
trimer, gel filtration | Pyrococcus furiosus |
| 66000 | - |
after heating at 95°C in 1% SDS for 24 h, SDS-PAGE | Pyrococcus furiosus |
| 86000 | - |
SDS-PAGE, the protein is not fully denatured by SDS | Pyrococcus furiosus |
| 124000 | - |
hexamer, gel filtration | Pyrococcus furiosus |
Organic Solvent Stability
| Organic Solvent | Comment | Organism |
|---|---|---|
| 2-mercaptoethanol | the protease retains some proteolytic activity in the presence of 2-mercaptoethanol | Pyrococcus furiosus |
| dithiothreitol | the protease retains some proteolytic activity in the presence of dithiothreitol | Pyrococcus furiosus |
| SDS | the protease is resistant to SDS | Pyrococcus furiosus |
| urea | the protease is resistant to urea | Pyrococcus furiosus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pyrococcus furiosus | Q51732 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Pyrococcus furiosus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| azocasein + H2O | - |
Pyrococcus furiosus | ? | - |
? | |
| Gelatin + H2O | - |
Pyrococcus furiosus | ? | - |
? | |
| Suc-Ala-Ala-Phe-NHMec + H2O | pH 7, NHMec = 7-amino-4-methylcoumaryl | Pyrococcus furiosus | Suc-Ala-Ala-Phe + 7-amino-4-methylcoumarin | NHMec = 7-amino-4-methylcoumaryl | ? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| hexamer | 6 * 18800, gel filtration | Pyrococcus furiosus |
| trimer | 3 * 18800, gel filtration | Pyrococcus furiosus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| PfpI endopeptidase | - |
Pyrococcus furiosus |
| PfpI protease | - |
Pyrococcus furiosus |
| Pyrococcus furiosus protease I | - |
Pyrococcus furiosus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 86 | - |
- |
Pyrococcus furiosus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 98 | - |
the enzyme has a 33 h half-life at 98°C after heating in 1% SDS | Pyrococcus furiosus |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Pyrococcus furiosus | trimer, experimentally determined | 3.9 | 3.7 |
| Pyrococcus furiosus | 18.8 kDa subunit, calculated from amino acid sequence | - |
6.1 |
| Pyrococcus furiosus | hexamer, experimentally determined | - |
6.1 |
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