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Literature summary for 3.4.22.B20 extracted from

  • Halio, S.B.; Blumentals, II; Short, S.A.; Merrill, B.M.; Kelly, R.M.
    Sequence, expression in Escherichia coli, and analysis of the gene encoding a novel intracellular protease (PfpI) from the hyperthermophilic archaeon Pyrococcus furiosus (1996), J. Bacteriol., 178, 2605-2612.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.4.22.B20

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Pyrococcus furiosus

Organism

Organism UniProt Comment Textmining
Pyrococcus furiosus Q51732
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Ala-Ala-Phe-7-amido-4-methylcoumarin
-
 Pyrococcus furiosus Ala-Ala-Phe + 7-amino-4-methylcoumarin
-
 ?

Subunits

Subunits Comment Organism
? x + 18800, homomultimer, SDS-PAGE Pyrococcus furiosus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
95
-
 reaction with Ala-Ala-Phe-7-amido-4-methylcoumarinamide, recombinant enzyme Pyrococcus furiosus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
70 100 70°C: about 70% of maximal activity, 100°C: about 75% of maximal activity, reaction with Ala-Ala-Phe-7-amido-4-methylcoumarinamide, recombinant enzyme Pyrococcus furiosus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
95
-
 in presence of 2% SDS and 80 nM dithiothreitol does not dissociate the protein, half-life: 19 min Pyrococcus furiosus