3.4.22.B20: PfpI endopeptidase
This is an abbreviated version!
For detailed information about PfpI endopeptidase, go to the full flat file.
Reaction
efficient cleavage of Ala-Ala-Phe-/-7-amido-4-methylcoumarinamide. Dominant protease in Pyrococcus furiosus =
Synonyms
C56.001, PfpI, PfpI endopeptidase, PfpI protease, Pyrococcus furiosus protease I
ECTree
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Substrates Products
Substrates Products on EC 3.4.22.B20 - PfpI endopeptidase
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REACTION DIAGRAM
Ala-Ala-Phe-7-amido-4-methylcoumarin
Ala-Ala-Phe + 7-amino-4-methylcoumarin
-
-
-
?
Ala-Phe-Lys-7-amido-4-methylcoumarin
Ala-Phe-Lys + 7-amino-4-methylcoumarin
-
-
-
?
Leu-Tyr-7-amido-4-methylcoumarin
Leu-Tyr + 7-amino-4-methylcoumarin
-
-
-
?
Suc-Ala-Ala-Phe-NHMec + H2O
Suc-Ala-Ala-Phe + 7-amino-4-methylcoumarin
pH 7, NHMec = 7-amino-4-methylcoumaryl
NHMec = 7-amino-4-methylcoumaryl
-
?