3.4.22.B20: PfpI endopeptidase
This is an abbreviated version!
For detailed information about PfpI endopeptidase, go to the full flat file.
Reaction
efficient cleavage of Ala-Ala-Phe-/-7-amido-4-methylcoumarinamide. Dominant protease in Pyrococcus furiosus =
Synonyms
C56.001, PfpI, PfpI endopeptidase, PfpI protease, Pyrococcus furiosus protease I
ECTree
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Temperature Range
Temperature Range on EC 3.4.22.B20 - PfpI endopeptidase
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50 - 110
50°C: about 55% of maximal activity, 110°C: about 45% of maximal activity, enzyme form PfpI-C1, reaction with Ala-Ala-Phe-7-amido-4-methylcoumarinamide
70 - 100
70°C: about 70% of maximal activity, 100°C: about 75% of maximal activity, reaction with Ala-Ala-Phe-7-amido-4-methylcoumarinamide, recombinant enzyme