Activating Compound | Comment | Organism | Structure |
---|---|---|---|
N-lauryl sarcosine | 0.01% v/v, increases proteolytic activity by about 50% | Pyrococcus furiosus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
intracellular | - |
Pyrococcus furiosus | 5622 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
NaCl | 100 mM, increases proteolytic activity by about 50% | Pyrococcus furiosus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
18800 | - |
3 * 18800, enzyme form PfpI-C2, SDS-PAGE | Pyrococcus furiosus |
18800 | - |
6 * 18800, enzyme form PfpI-C1, SDS-PAGE | Pyrococcus furiosus |
59000 | - |
PfpI-C2, gel filtration | Pyrococcus furiosus |
124000 | - |
PfpI-C1, gel filtration | Pyrococcus furiosus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pyrococcus furiosus | Q51732 | - |
- |
Purification (Comment) | Organism |
---|---|
purification of two enzyme forms: PfpI-C1 and PfpI-C2 | Pyrococcus furiosus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Pyrococcus furiosus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Ala-Ala-Phe-7-amido-4-methylcoumarin | - |
Pyrococcus furiosus | Ala-Ala-Phe + 7-amino-4-methylcoumarin | - |
? | |
Ala-Phe-Lys-7-amido-4-methylcoumarin | - |
Pyrococcus furiosus | Ala-Phe-Lys + 7-amino-4-methylcoumarin | - |
? | |
Leu-Tyr-7-amido-4-methylcoumarin | - |
Pyrococcus furiosus | Leu-Tyr + 7-amino-4-methylcoumarin | - |
? |
Subunits | Comment | Organism |
---|---|---|
hexamer | 6 * 18800, enzyme form PfpI-C1, SDS-PAGE | Pyrococcus furiosus |
trimer | 3 * 18800, enzyme form PfpI-C2, SDS-PAGE | Pyrococcus furiosus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
84 | - |
enzyme form PfpI-C1, reaction with Leu-Tyr-7-amido-4-methylcoumarinamide | Pyrococcus furiosus |
86 | - |
enzyme form PfpI-C1, reaction with Ala-Ala-Phe-7-amido-4-methylcoumarinamide | Pyrococcus furiosus |
97 | - |
enzyme form PfpI-C1, reaction with Ala-Phe-Lys-7-amido-4-methylcoumarinamide | Pyrococcus furiosus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | 110 | 50°C: about 55% of maximal activity, 110°C: about 45% of maximal activity, enzyme form PfpI-C1, reaction with Ala-Ala-Phe-7-amido-4-methylcoumarinamide | Pyrococcus furiosus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
90 | - |
half life is 45 min, in presence of 0.5 M KHPO4 the half-life is extended to 80 min, activity with Ala-Ala-Phe-7-amido-4-methylcoumarinamide | Pyrococcus furiosus |
95 | - |
5 min, no inactivation | Pyrococcus furiosus |
98 | - |
half life is below 30 min, in presence of 0.8 M KHPO4 the half-life is extended to 110 min, activity with Ala-Ala-Phe-7-amido-4-methylcoumarinamide | Pyrococcus furiosus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.3 | - |
enzyme form PfpI-C1, reaction with Ala-Ala-Phe-7-amido-4-methylcoumarinamide | Pyrococcus furiosus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.2 | 8.5 | pH 5.2: about 60% of maximal activity, pH 8.5: about 50% of maximal activity, enzyme form PfpI-C1, reaction with Ala-Ala-Phe-7-amido-4-methylcoumarinamide | Pyrococcus furiosus |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Pyrococcus furiosus | enzyme form PfpI-C1 | - |
6.1 |