3.4.22.B20: PfpI endopeptidase
This is an abbreviated version!
For detailed information about PfpI endopeptidase, go to the full flat file.
Reaction
efficient cleavage of Ala-Ala-Phe-/-7-amido-4-methylcoumarinamide. Dominant protease in Pyrococcus furiosus
=
Synonyms
C56.001, PfpI, PfpI endopeptidase, PfpI protease, Pyrococcus furiosus protease I
ECTree
Molecular Weight
Molecular Weight on EC 3.4.22.B20 - PfpI endopeptidase
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66000
after heating at 95°C in 1% SDS for 24 h, SDS-PAGE
86000
SDS-PAGE, the protein is not fully denatured by SDS
124000
PfpI-C1, gel filtration
124000
hexamer, gel filtration
18800
monomer, calculated from amino acid sequence
18800
3 * 18800, enzyme form PfpI-C2, SDS-PAGE
18800
6 * 18800, enzyme form PfpI-C1, SDS-PAGE
59000
PfpI-C2, gel filtration
59000
trimer, gel filtration