3.4.22.8: clostripain
This is an abbreviated version!
For detailed information about clostripain, go to the full flat file.
Reaction
Preferential cleavage: Arg-/-, including Arg-/-Pro, but not Lys- =
Synonyms
alpha-clostridipain, alpha-clostripain, CLO, clostridiopeptidase, clostridiopeptidase B, Clostridium histolyticum proteinase B, clostripain-like protease, EC 3.4.4.20, proteinase, Clostridium histolyticum, B
ECTree
3.4.22.8 clostripainAdvanced search results
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results (Specific Activity
Specific Activity on EC 3.4.22.8 - clostripain
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SPECIFIC ACTIVITY [µmol/min/mg] 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
0.022
commercial enzyme preparation, using acyl-L-lysine-4-nitroanilide as substrate, in 50 mM Tris-HCl, 5 mM dithiothreitol, and 5 mM CaCl2, at pH 7.5 and 25°C
0.039
purified recombinant enzyme, using acyl-L-lysine-4-nitroanilide as substrate, in 50 mM Tris-HCl, 5 mM dithiothreitol, and 5 mM CaCl2, at pH 7.5 and 25°C
0.119
culture supernatant, using Nalpha-benzoyl-L-arginine-4-nitroanilide as substrate, in 50 mM Tris-HCl, 5 mM dithiothreitol, and 5 mM CaCl2, at pH 7.5 and 25°C
61.2
recombinant enzyme after 515fold purification, using Nalpha-benzoyl-L-arginine-4-nitroanilide as substrate, in 50 mM Tris-HCl, 5 mM dithiothreitol, and 5 mM CaCl2, at pH 7.5 and 25°C
additional information
