3.4.22.8: clostripain

This is an abbreviated version!
For detailed information about clostripain, go to the full flat file.

Reaction

Preferential cleavage: Arg-/-, including Arg-/-Pro, but not Lys- =

Synonyms

alpha-clostridipain, alpha-clostripain, CLO, clostridiopeptidase, clostridiopeptidase B, Clostridium histolyticum proteinase B, clostripain-like protease, EC 3.4.4.20, proteinase, Clostridium histolyticum, B

ECTree

     3 Hydrolases

         3.4 Acting on peptide bonds (peptidases)

             3.4.22 Cysteine endopeptidases

                3.4.22.8 clostripain

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Results	in table
187	AA Sequence
7	Application
1	CAS Registry Number
8	Cloned(Commentary)
4	General Information
1	General Stability
16	IC50 Value
85	Inhibitors
4	kcat/KM [mM/s]
2	Ki Value [mM]
22	KM Value [mM]
1	Localization
3	Metals/Ions
16	Molecular Weight [Da]
42	Organism
9	pH Optimum
3	pH Range
1	Posttranslational Modification
2	Protein Variants
11	Purification (Commentary)
1	Reaction
2	Reaction Type
42	Reference
12	Specific Activity [micromol/min/mg]
84	Substrates and Products (Substrate)
12	Subunits
12	Synonyms
1	Temperature Stability [°C]
16	Turnover Number [1/s]

KM Value

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KM Value on EC 3.4.22.8 - clostripain

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KM VALUE [mM]

SUBSTRATE

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

IMAGE

25.4

Ac-L-Lys-4-nitroanilide

Clostridium perfringens

-

-

709851

0.174 - 0.26

alpha-benzoyl-Arg ethyl ester

4 entries

0.16 - 0.22

benzoyl-Arg ethyl ester

2 entries

0.852

benzoyl-L-Arg-4-nitroanilide

Clostridium perfringens

-

-

709851

0.36

maleyl-Tyr-Arg ethyl ester

Hathewaya histolytica

-

-

81595

1.3

N-benzoyl-L-Arg amide

Hathewaya histolytica

-

-

81578, 81588

0.3 - 0.34

Nalpha-benzoyl-L-arginine-4-nitroanilide

2 entries

3

Nalpha-benzoyl-L-Lys methyl ester

Hathewaya histolytica

-

-

81588

0.25

Nalpha-p-tosyl-L-Arg amide

Hathewaya histolytica

-

-

81588

0.26 - 2.5

p-Nitrophenyl p-guanidinobenzoate

2 entries

0.022 - 0.8

p-toluenesulfonyl-L-Arg methyl ester

2 entries

0.25

Thr-Ala-Ala

Hathewaya histolytica

-

-

81578

additional information

additional information

Hathewaya histolytica

-

effect of pH-value on Km-value for the hydrolysis of alpha-benzoyl-Arg ethyl ester, effect of temperature on Km-values

81588

-

0.174

alpha-benzoyl-Arg ethyl ester

Hathewaya histolytica

-

-

81587

0.235

alpha-benzoyl-Arg ethyl ester

Hathewaya histolytica

-

-

81584

0.24 - 0.25

alpha-benzoyl-Arg ethyl ester

Hathewaya histolytica

-

depending on assay method

81588

0.26

alpha-benzoyl-Arg ethyl ester

Hathewaya histolytica

-

-

81582

0.16

benzoyl-Arg ethyl ester

Hathewaya histolytica

-

-

81595

0.22

benzoyl-Arg ethyl ester

Hathewaya histolytica

-

-

81578

0.3

Nalpha-benzoyl-L-arginine-4-nitroanilide

Hathewaya histolytica

P09870

purified recombinant enzyme, in 50 mM Tris-HCl, 5 mM dithiothreitol, and 5 mM CaCl2, at pH 7.5 and 25°C

718358

0.34

Nalpha-benzoyl-L-arginine-4-nitroanilide

Hathewaya histolytica

P09870

commercial enzyme preparation, in 50 mM Tris-HCl, 5 mM dithiothreitol, and 5 mM CaCl2, at pH 7.5 and 25°C

718358

0.26

p-Nitrophenyl p-guanidinobenzoate

Hathewaya histolytica

-

-

81588

2.5

p-Nitrophenyl p-guanidinobenzoate

Hathewaya histolytica

-

-

81578

0.022

p-toluenesulfonyl-L-Arg methyl ester

Hathewaya histolytica

-

-

81578, 81588

0.8

p-toluenesulfonyl-L-Arg methyl ester

Hathewaya histolytica

-

-

81588