3.4.22.8: clostripain
This is an abbreviated version!
For detailed information about clostripain, go to the full flat file.
Reaction
Preferential cleavage: Arg-/-, including Arg-/-Pro, but not Lys- =
Synonyms
alpha-clostridipain, alpha-clostripain, CLO, clostridiopeptidase, clostridiopeptidase B, Clostridium histolyticum proteinase B, clostripain-like protease, EC 3.4.4.20, proteinase, Clostridium histolyticum, B
ECTree
Advanced search results
Application
Application on EC 3.4.22.8 - clostripain
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
medicine
synthesis
-
influence of protease inhibitors aprotinin, phenylmethylsulfonyl fluoride, L-1-chloro-3-[4-tosylamido]-7-amino-2-heptanone, and chymostatin on toxicity of Clostridium histolyticum supernatant towards HeLa cells. Aprotinin has no effect, while the other inhibitors potentiate the cytotoxic activity of Clostridium histolyticum probybly by hindering the natural defense of cells. In addition, phenylmethylsulfonyl fluoride and L-1-chloro-3-[4-tosylamido]-7-amino-2-heptanone block clostripain enzymic activity
medicine
it is possible to isolate significant numbers of human islets combining collagenase only with clostripain. The supplementation with clostripain is an effective means to substantially reduce neutral protease activity, which significantly decreases survival and viability after culture
medicine
neutral protease derived from Clostridium histolyticum and clostripain, in combination with collagenase derived from the same bacteria, may effectively increase the isolation efficiency of pancreatic islet without affecting the quality of islets
medicine
the addition of clostripain to the enzyme blend soundly improves islet yields and transplantation rates. It gently aids pancreas digestion and maintained proper islet functionality. The addition of clostripain to the enzyme blend is implemented into standard isolation protocols at the isolation centers in Uppsala and in Oslo
-
the enzyme can be used for the high yielding synthesis of a variety of peptide bond using L-Arg and, to a lesser degree, L-Lys as substrates with L-amino acid amides or D-amino acid amides
synthesis
-
efficient biocatalyst for the synthesis of peptide isosteres
synthesis
-
recombinant clostripain might prove useful in the production of insulin from the proinsulin fusion protein