Information on EC 3.4.22.8 - clostripain

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The expected taxonomic range for this enzyme is: Clostridium

EC NUMBER
COMMENTARY hide
3.4.22.8
-
RECOMMENDED NAME
GeneOntology No.
clostripain
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
Preferential cleavage: Arg-/-, including Arg-/-Pro, but not Lys-
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
esterification
-
-
-
-
hydrolysis of peptide bond
-
-
endpeptidase
-
CAS REGISTRY NUMBER
COMMENTARY hide
9028-00-6
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
Clostridium histolyticum
Clostridium histolyticum ATCC 17859
-
-
-
Manually annotated by BRENDA team
Clostridium histolyticum ATCC 17860
-
-
-
Manually annotated by BRENDA team
Clostridium histolyticum ATCC 19401
Clostridium histolyticum ATCC 25770
-
-
-
Manually annotated by BRENDA team
Clostridium histolyticum ATCC 6282
-
-
-
Manually annotated by BRENDA team
Clostridium histolyticum ATCC 8034
-
-
-
Manually annotated by BRENDA team
strain 13
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
physiological function
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
Ac-L-Lys-4-nitroanilide + H2O
N2-acetyl-L-Lys + 4-nitroaniline
show the reaction diagram
acyl-L-lysine-4-nitroanilide + H2O
acyl-L-lysine + 4-nitroaniline
show the reaction diagram
azocasein + H2O
?
show the reaction diagram
benzoyl-Arg ethyl ester + 3-amino-propionamide
ethanol + benzoyl-Arg-3-amino-propionamide
show the reaction diagram
Clostridium histolyticum
-
-
-
?
benzoyl-Arg ethyl ester + 3-amino-propionic acid methyl ester
ethanol + benzoyl-Arg-3-amino-propionic acid methyl ester
show the reaction diagram
Clostridium histolyticum
-
-
-
?
benzoyl-Arg ethyl ester + 4-amino-butyramide
ethanol + benzoyl-Arg-4-amino-butyramide
show the reaction diagram
Clostridium histolyticum
-
-
-
?
benzoyl-Arg ethyl ester + 4-amino-butyric acid methyl ester
ethanol + benzoyl-Arg-4-amino-butyric acid methyl ester
show the reaction diagram
Clostridium histolyticum
-
-
-
?
benzoyl-Arg ethyl ester + 5-amino-pentanoic acid
ethanol + benzoyl-Arg-5-amino-pentanoic acid
show the reaction diagram
Clostridium histolyticum
-
-
-
?
benzoyl-Arg ethyl ester + 5-amino-pentanoic acid amide
ethanol + benzoyl-Arg-5-amino-pentanoic acid amide
show the reaction diagram
Clostridium histolyticum
-
-
-
?
benzoyl-Arg ethyl ester + 5-amino-pentanoic acid methyl ester
ethanol + benzoyl-Arg-5-amino-pentanoic acid methyl ester
show the reaction diagram
Clostridium histolyticum
-
-
-
?
benzoyl-Arg ethyl ester + 6-amino-hexanoic acid
ethanol + benzoyl-Arg-6-amino-hexanoic acid
show the reaction diagram
Clostridium histolyticum
-
-
-
?
benzoyl-Arg ethyl ester + 6-amino-hexanoic acid amide
ethanol + benzoyl-Arg-6-amino-hexanoic acid amide
show the reaction diagram
Clostridium histolyticum
-
-
-
?
benzoyl-Arg ethyl ester + 6-amino-hexanoic acid methyl ester
ethanol + benzoyl-Arg-6-amino-hexanoic acid methyl ester
show the reaction diagram
Clostridium histolyticum
-
-
-
?
benzoyl-Arg ethyl ester + 9-amino-nonanoic acid
ethanol + benzoyl-Arg-9-amino-nonanoic acid
show the reaction diagram
Clostridium histolyticum
-
-
-
?
benzoyl-Arg ethyl ester + 9-amino-nonanoic acid amide
ethanol + benzoyl-Arg-9-amino-nonanoic acid amide
show the reaction diagram
Clostridium histolyticum
-
-
-
?
benzoyl-Arg ethyl ester + 9-amino-nonanoic acid methyl ester
ethanol + benzoyl-Arg-9-amino-nonanoic acid methyl ester
show the reaction diagram
Clostridium histolyticum
-
-
-
?
benzoyl-L-Arg-4-nitroanilide + H2O
benzoyl-L-Arg + 4-nitroaniline
show the reaction diagram
benzoyl-Lys-4-nitroanilide + H2O
benzoyl-Lys + 4-nitroaniline
show the reaction diagram
benzoyl-Phe 4-guanidinephenyl ester + 2-amino-4-methyl-pentan-1-ol
4-guanidinephenol + benzoyl-Phe-2-amino-4-methyl-pentan-1-ol
show the reaction diagram
Clostridium histolyticum
-
-
-
?
benzoyl-Phe 4-guanidinephenyl ester + 3-amino-propane-1,2-diol
4-guanidinephenol + benzoyl-Phe-3-amino-propane-1,2-diol
show the reaction diagram
Clostridium histolyticum
-
-
-
?
benzoyl-Phe 4-guanidinephenyl ester + 3-amino-propanol
4-guanidinephenol + benzoyl-Phe-3-amino-propanol
show the reaction diagram
Clostridium histolyticum
-
-
-
?
benzoyl-Phe 4-guanidinephenyl ester + 5-amino-pentanol
4-guanidinephenol + benzoyl-Phe-5-amino-pentanol
show the reaction diagram
Clostridium histolyticum
-
-
-
?
benzoyl-Phe 4-guanidinephenyl ester + cycloheptylamine
4-guanidinephenol + benzoyl-Phe-cycloheptylamide
show the reaction diagram
Clostridium histolyticum
-
-
-
?
benzoyl-Phe 4-guanidinephenyl ester + pentylamine
4-guanidinephenol + benzoyl-Phe-pentylamide
show the reaction diagram
Clostridium histolyticum
-
-
-
?
benzoyl-Phe 4-guanidinephenyl ester + propylamine
4-guanidinephenol + benzoyl-Phe-propylamide
show the reaction diagram
Clostridium histolyticum
-
-
-
?
carbobenzoxy-Arg methyl ester + L-Pro-NH2
carbobenzoxy-Arg-Pro-NH2 + methanol
show the reaction diagram
Clostridium histolyticum
-
no activity with carbobenzoxy-D-Arg methyl ester
-
?
carbobenzoxy-L-Arg methyl ester + D-Phe-NH2
carbobenzoxy-Arg-D-Phe-NH2 + methanol
show the reaction diagram
Clostridium histolyticum
-
no activity with carbobenzoxy-D-Arg methyl ester
-
-
?
carbobenzoxy-L-Arg methyl ester + D-Trp-NH2
carbobenzoxy-Arg-D-Trp-NH2 + methanol
show the reaction diagram
Clostridium histolyticum
-
no activity with carbobenzoxy-D-Arg methyl ester
-
-
?
carbobenzoxy-L-Arg methyl ester + Gly-NH2
carbobenzoxy-Arg-Gly-NH2 + methanol
show the reaction diagram
Clostridium histolyticum
-
no activity with carbobenzoxy-D-Arg methyl ester
-
-
?
carbobenzoxy-L-Arg methyl ester + L-Phe-NH2
carbobenzoxy-Arg-Phe-NH2 + methanol
show the reaction diagram
Clostridium histolyticum
-
no activity with carbobenzoxy-D-Arg methyl ester
-
-
?
carbobenzoxy-L-Arg methyl ester + L-Trp-NH2
carbobenzoxy-Arg-Trp-NH2 + methanol
show the reaction diagram
Clostridium histolyticum
-
no activity with carbobenzoxy-D-Arg methyl ester
-
-
?
cholecystokinin + H2O
cholecystokinin 8
show the reaction diagram
Clostridium histolyticum
-
cleavage of large forms of cholecystokinin
-
?
histone H3 + H2O
?
show the reaction diagram
Clostridium histolyticum
-
-
-
-
?
histone H4 + H2O
?
show the reaction diagram
Clostridium histolyticum
-
-
-
-
?
L-Arg methyl ester + H2O
L-Arg + methanol
show the reaction diagram
Clostridium histolyticum
-
-
-
-
?
maleyl-Tyr-Arg ethyl ester + H2O
?
show the reaction diagram
Clostridium histolyticum
-
-
-
-
?
N-benzoyl-Arg amide + H2O
N-benzoyl-Arg + NH3
show the reaction diagram
Clostridium histolyticum
-
-
-
-
?
N-benzoyl-Arg benzyl ester + H2O
N-benzoyl-Arg + benzyl alcohol
show the reaction diagram
Clostridium histolyticum
-
-
-
-
?
N-benzoyl-Arg ethyl ester + H2O
N-benzoyl-Arg + ethanol
show the reaction diagram
Clostridium histolyticum
-
-
-
-
?
N-benzoyl-Arg isopropyl ester + H2O
N-benzoyl-Arg isopropanol
show the reaction diagram
Clostridium histolyticum
-
-
-
-
?
N-benzoyl-Arg methyl ester + H2O
N-benzoyl-Arg + methanol
show the reaction diagram
Clostridium histolyticum
-
-
-
-
?
N-benzoyl-Arg naphthylamide + H2O
N-benzoyl-Arg + naphthylamine
show the reaction diagram
Clostridium histolyticum
-
-
-
-
?
N-benzoyl-DL-arginine 4-nitroanilide + H2O
N-benzoyl-DL-arginine + 4-nitroaniline
show the reaction diagram
Clostridium histolyticum
-
-
-
?
N-Benzoyl-L-Arg ethyl ester + H2O
N-Benzoyl-L-Arg + ethanol
show the reaction diagram
Clostridium histolyticum
-
-
-
?
Nalpha-benzoyl-Arg p-nitroanilide + H2O
Nalpha-benzoyl-Arg + p-nitroaniline
show the reaction diagram
Clostridium histolyticum
-
-
-
-
?
Nalpha-benzoyl-DL-arginine-4-nitroanilide + H2O
Nalpha-benzoyl-DL-arginine + 4-nitroaniline
show the reaction diagram
Clostridium histolyticum
-
-
-
-
?
Nalpha-benzoyl-L-arginine ethyl ester + H2O
?
show the reaction diagram
Nalpha-benzoyl-L-arginine-4-nitroanilide + H2O
Nalpha-benzoyl-L-arginine + 4-nitroaniline
show the reaction diagram
Nalpha-benzoyl-Lys methyl ester + H2O
Nalpha-benzoyl-Lys + methanol
show the reaction diagram
Clostridium histolyticum
-
-
-
-
?
Nalpha-p-tosyl-Arg amide + H2O
Nalpha-p-tosyl-Arg + NH3
show the reaction diagram
Clostridium histolyticum
-
-
-
-
?
p-nitrophenyl p-guanidinobenzoate + H2O
p-nitrophenol + p-guanidinobenzoate
show the reaction diagram
p-tosyl-Arg methyl ester + H2O
p-tosyl-Arg + methanol
show the reaction diagram
parvalbumin + H2O
?
show the reaction diagram
Clostridium histolyticum
-
exclusive cleavage of the Arg75-Ala76 bond
-
-
?
proinsulin fusion protein + H2O
insulin + ?
show the reaction diagram
-
clostripain specifically cleaves the arginine residues in the leader peptide and in the C-peptide
-
-
?
succinyl-CoA synthetase + H2O
?
show the reaction diagram
Clostridium histolyticum
-
the mutant forms of E. coli succinyl-CoA synthetase, W76F and W43,76,248F are more sensitive to proteolysis by clostripain than the wild-type enzyme. No influence of phosphorylation or dephosphorylation state. Argbeta80 is the principal site of inactivation by clostripain
-
-
?
Thr-Ala-Ala + H2O
?
show the reaction diagram
Clostridium histolyticum
-
-
-
-
-
additional information
?
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
5,5'-dithiobis(2-nitrobenzoate)
Clostridium histolyticum
-
-
ADP
Clostridium histolyticum
-
proteolysis of succinyl-CoA synthetase
antipain
Clostridium histolyticum
-
potent clostridiopeptidase B-induced kinin release in dog plasma
Aprotinin
Clostridium histolyticum
-
-
ATP
Clostridium histolyticum
-
proteolysis of succinyl-CoA synthetase
benzamidine
benzylamidinyl-diazo dichlorotriazine
Clostridium histolyticum
-
effective protection by N-benzoyl-L-Arg ethyl ester
benzyloxycarbonyl-Phe-Lys-CH2S(CH3)2
Clostridium histolyticum
-
irreversible
Borate
Clostridium histolyticum
-
partial inhibition
Butylamine
Clostridium histolyticum
-
-
butylguanidine
citrate
Clostridium histolyticum
-
partial inhibition
diallyl-2,4,4,9-tetramethyl-2,3,4,5-tetrahydro-1H-benzo[b][1,4]diazepin-2-ylphosphonate
Clostridium histolyticum
-
-
diallyl-2,4,4-trimethyl-2,3,4,5-tetrahydro-1H-benzo[b][1,4]diazepin-2-ylphosphonate
Clostridium histolyticum
-
-
diallyl-7,8-dichloro-2,4,4-trimethyl-2,3,4,5-tetrahydro-1H-benzo[b][1,4]diazepin-2-ylphosphonate
Clostridium histolyticum
-
-
diallyl-7-benzoyl-2,4,4-trimethyl-2,3,4,5-tetrahydro-1H-benzo[b][1,4]diazepin-2-ylphosphonate
Clostridium histolyticum
-
-
dibutyl-2,4,4,9-tetramethyl-2,3,4,5-tetrahydro-1H-benzo[b][1,4]diazepin-2-ylphosphonate
Clostridium histolyticum
-
-
dibutyl-2,4,4-trimethyl-2,3,4,5-tetrahydro-1H-benzo[b][1,4]diazepin-2-ylphosphonate
Clostridium histolyticum
-
-
dibutyl-7,8-dichloro-2,4,4-trimethyl-2,3,4,5-tetrahydro-1H-benzo[b][1,4]diazepin-2-ylphosphonate
Clostridium histolyticum
-
-
dibutyl-7-benzoyl-2,4,4-trimethyl-2,3,4,5-tetrahydro-1H-benzo-[b][1,4]diazepin-2-ylphosphonate
Clostridium histolyticum
-
-
diethyl-2,4,4,9-tetramethyl-2,3,4,5-tetrahydro-1H-benzo[b][1,4]diazepin-2-ylphosphonate
Clostridium histolyticum
-
-
diethyl-2,4,4-trimethyl-8-nitro-2,3,4,5-tetrahydro-1H-benzo-[b][1,4]diazepin-2-ylphosphonate
Clostridium histolyticum
-
-
diethyl-7,8-dichloro-2,4,4-trimethyl-2,3,4,5-tetrahydro-1H-benzo[b][1,4]diazepin-2-ylphosphonate
Clostridium histolyticum
-
-
diethyl-7-benzoyl-2,4,4-trimethyl-2,3,4,5-tetrahydro-1H-benzo-[b][1,4]diazepin-2-ylphosphonate
Clostridium histolyticum
-
-
diethyldicarbonate
Clostridium histolyticum
-
-
diisopropylfluorophosphate
Clostridium histolyticum
-
-
dimethyl-2,4,4-trimethyl-2,3,4,5-tetrahydro-1H-benzo-[b]-[1,4]diazepin-2-ylphosphonate
Clostridium histolyticum
-
-
dimethyl-2,4,4-trimethyl-8-nitro-2,3,4,5-tetrahydro-1H-benzo-[b][1,4]diazepin-2-ylphosphonate
Clostridium histolyticum
-
-
dimethyl-7,8-dichloro-2,4,4-trimethyl-2,3,4,5-tetrahydro-1H-benzo[b][1,4]diazepin-2-ylphosphonate
Clostridium histolyticum
-
-
dimethyl-7-benzoyl-2,4,4-trimethyl-2,3,4,5-tetrahydro-1H-benzo[b][1,4]diazepin-2-ylphosphonate
Clostridium histolyticum
-
-
E-64
Clostridium histolyticum
-
0.035 mM, 68% inhibition
EDTA
Clostridium histolyticum
-
-
ethylamine
Clostridium histolyticum
-
-
Ethylguanidine
Clostridium histolyticum
-
-
histatin 5
Clostridium histolyticum
-
23.6 nM, 50% inhibition, competitive. The salivary protein could be used for the prevention or treatment of topical infactions such as those caused by Clostridium histolyticum
-
iodoacetamide
Clostridium histolyticum
-
-
iodoacetate
Clostridium histolyticum
-
-
iodoacetic acid
Clostridium histolyticum
-
0.035 mM, 13% inhibition
L-1-chloro-3-[4-tosylamido]-7-amino-2-heptanone
Clostridium histolyticum
-
complete inhibition
L-3-carboxy-2,3-trans-epoxypropionyl-leucylamido-(4-guanidino)butane
Clostridium histolyticum
-
i.e. compounds E-64
Leupeptin
macroglobulin
Clostridium histolyticum
-
alpha2-macroglobulin from human plasma, inhibits both the amidase and protease activity. Macroglobulin from rat and dog plasma
-
methylamine
Clostridium histolyticum
-
-
Methylguanidine
Clostridium histolyticum
-
-
N-alpha-p-nitrobenzyloxycarbonyl-Arg chloromethyl ketone
Clostridium histolyticum
-
irreversible
N-Alpha-p-tosyl-L-homoarginine methyl ester
Clostridium histolyticum
-
-
N-alpha-p-tosyl-L-Lys chloromethyl ketone
N-Alpha-p-tosyl-L-norarginine methyl ester
Clostridium histolyticum
-
-
N-tosyl-Lys-chloromethylketone
Clostridium histolyticum
-
0.035 mM, complete inhibition
Nalpha-tosyl-L-lysine chloromethylketone
Clostridium histolyticum
-
complete inhibition at 0.01 mg/ml
ovoinhibitor
Clostridium histolyticum
-
0.05 mM, 55% reduced amidase activity
-
PCMB
Clostridium histolyticum
-
-
phenylmethylsulfonyl fluoride
Clostridium histolyticum
-
complete inhibition
Polyarginine
Clostridium histolyticum
-
hydrolysis of benzoyl-Arg ethyl ester
polylysine
Clostridium histolyticum
-
hydrolysis of benzoyl-Arg ethyl ester
Propylamine
Clostridium histolyticum
-
-
propylguanidine
Clostridium histolyticum
-
-
RCM RNase
Clostridium histolyticum
-
hydrolysis of benzoyl-Arg ethyl ester
-
tosyl-Lys-chloromethane
Clostridium histolyticum
-
-
Tris
Clostridium histolyticum
-
partial inhibition
Trypsin inhibitor
Veronal
Clostridium histolyticum
-
partial inhibition
additional information
Clostridium histolyticum
-
no inhibition by 0.035 PMSF
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
25.4
Ac-L-Lys-4-nitroanilide
-
-
0.174 - 0.26
alpha-benzoyl-Arg ethyl ester
0.16 - 0.22
benzoyl-Arg ethyl ester
0.852
benzoyl-L-Arg-4-nitroanilide
-
-
0.36
maleyl-Tyr-Arg ethyl ester
Clostridium histolyticum
-
-
1.3
N-benzoyl-L-Arg amide
Clostridium histolyticum
-
-
0.3 - 0.34
Nalpha-benzoyl-L-arginine-4-nitroanilide
3
Nalpha-benzoyl-L-Lys methyl ester
Clostridium histolyticum
-
-
0.25
Nalpha-p-tosyl-L-Arg amide
Clostridium histolyticum
-
-
0.26 - 2.5
p-Nitrophenyl p-guanidinobenzoate
0.022 - 0.8
p-toluenesulfonyl-L-Arg methyl ester
0.25
Thr-Ala-Ala
Clostridium histolyticum
-
-
additional information
additional information
Clostridium histolyticum
-
effect of pH-value on Km-value for the hydrolysis of alpha-benzoyl-Arg ethyl ester, effect of temperature on Km-values
-
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
22.53
Ac-L-Lys-4-nitroanilide
Clostridium perfringens
-
-
91 - 643
alpha-benzoyl-Arg ethyl ester
1170
benzoyl-Arg ethyl ester
Clostridium histolyticum
-
-
109.3
benzoyl-L-Arg-4-nitroanilide
Clostridium perfringens
-
-
824
maleyl-Tyr-Arg ethyl ester
Clostridium histolyticum
-
-
87
N-benzoyl-L-Arg amide
Clostridium histolyticum
-
-
38.8 - 69.5
Nalpha-benzoyl-L-arginine-4-nitroanilide
22
Nalpha-benzoyl-L-Lys methyl ester
Clostridium histolyticum
-
-
10
Nalpha-p-tosyl-L-Arg amide
Clostridium histolyticum
-
-
9.8
Nalpha-p-tosyl-L-Arg methyl ester
Clostridium histolyticum
-
-
31
p-Nitrophenyl p-guanidinobenzoate
Clostridium histolyticum
-
-
86
p-toluenesulfonyl-L-Arg methyl ester
Clostridium histolyticum
-
-
additional information
additional information
Clostridium histolyticum
-
effect of pH-value on the turnover-number for hydrolysis of alpha-benzoyl-Arg ethyl ester, effect of temperature on turnover numbers
-
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.887
Ac-L-Lys-4-nitroanilide
Clostridium perfringens
-
-
40630
128.3
benzoyl-L-Arg-4-nitroanilide
Clostridium perfringens
-
-
24211
116 - 234
Nalpha-benzoyl-L-arginine-4-nitroanilide
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.01
Co2+
Clostridium histolyticum
-
-
0.00001
histatin 5
Clostridium histolyticum
-
pH 7.4, 25C
-
IC50 VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.15
diallyl-2,4,4,9-tetramethyl-2,3,4,5-tetrahydro-1H-benzo[b][1,4]diazepin-2-ylphosphonate
Clostridium histolyticum
-
in 100 mM Tris-HCl buffer, pH 7.4, at 37C
0.032
diallyl-2,4,4-trimethyl-2,3,4,5-tetrahydro-1H-benzo[b][1,4]diazepin-2-ylphosphonate
Clostridium histolyticum
-
in 100 mM Tris-HCl buffer, pH 7.4, at 37C
0.09
diallyl-7,8-dichloro-2,4,4-trimethyl-2,3,4,5-tetrahydro-1H-benzo[b][1,4]diazepin-2-ylphosphonate
Clostridium histolyticum
-
in 100 mM Tris-HCl buffer, pH 7.4, at 37C
0.036
diallyl-7-benzoyl-2,4,4-trimethyl-2,3,4,5-tetrahydro-1H-benzo[b][1,4]diazepin-2-ylphosphonate
Clostridium histolyticum
-
in 100 mM Tris-HCl buffer, pH 7.4, at 37C
0.175
dibutyl-2,4,4,9-tetramethyl-2,3,4,5-tetrahydro-1H-benzo[b][1,4]diazepin-2-ylphosphonate
Clostridium histolyticum
-
in 100 mM Tris-HCl buffer, pH 7.4, at 37C
0.3
dibutyl-2,4,4-trimethyl-2,3,4,5-tetrahydro-1H-benzo[b][1,4]diazepin-2-ylphosphonate
Clostridium histolyticum
-
IC50 above 0.3 mM, in 100 mM Tris-HCl buffer, pH 7.4, at 37C
0.14
dibutyl-7,8-dichloro-2,4,4-trimethyl-2,3,4,5-tetrahydro-1H-benzo[b][1,4]diazepin-2-ylphosphonate
Clostridium histolyticum
-
in 100 mM Tris-HCl buffer, pH 7.4, at 37C
0.3
dibutyl-7-benzoyl-2,4,4-trimethyl-2,3,4,5-tetrahydro-1H-benzo-[b][1,4]diazepin-2-ylphosphonate
Clostridium histolyticum
-
IC50 above 0.3 mM, in 100 mM Tris-HCl buffer, pH 7.4, at 37C
0.08
diethyl-2,4,4,9-tetramethyl-2,3,4,5-tetrahydro-1H-benzo[b][1,4]diazepin-2-ylphosphonate
Clostridium histolyticum
-
in 100 mM Tris-HCl buffer, pH 7.4, at 37C
0.49
diethyl-2,4,4-trimethyl-8-nitro-2,3,4,5-tetrahydro-1H-benzo-[b][1,4]diazepin-2-ylphosphonate
Clostridium histolyticum
-
in 100 mM Tris-HCl buffer, pH 7.4, at 37C
0.22
diethyl-7,8-dichloro-2,4,4-trimethyl-2,3,4,5-tetrahydro-1H-benzo[b][1,4]diazepin-2-ylphosphonate
Clostridium histolyticum
-
in 100 mM Tris-HCl buffer, pH 7.4, at 37C
0.3
diethyl-7-benzoyl-2,4,4-trimethyl-2,3,4,5-tetrahydro-1H-benzo-[b][1,4]diazepin-2-ylphosphonate
Clostridium histolyticum
-
IC50 above 0.3 mM, in 100 mM Tris-HCl buffer, pH 7.4, at 37C
0.278
dimethyl-2,4,4-trimethyl-2,3,4,5-tetrahydro-1H-benzo-[b]-[1,4]diazepin-2-ylphosphonate
Clostridium histolyticum
-
in 100 mM Tris-HCl buffer, pH 7.4, at 37C
0.3
dimethyl-2,4,4-trimethyl-8-nitro-2,3,4,5-tetrahydro-1H-benzo-[b][1,4]diazepin-2-ylphosphonate
Clostridium histolyticum
-
IC50 above 0.3 mM, in 100 mM Tris-HCl buffer, pH 7.4, at 37C
0.78
dimethyl-7,8-dichloro-2,4,4-trimethyl-2,3,4,5-tetrahydro-1H-benzo[b][1,4]diazepin-2-ylphosphonate
Clostridium histolyticum
-
in 100 mM Tris-HCl buffer, pH 7.4, at 37C
0.07
dimethyl-7-benzoyl-2,4,4-trimethyl-2,3,4,5-tetrahydro-1H-benzo[b][1,4]diazepin-2-ylphosphonate
Clostridium histolyticum
-
in 100 mM Tris-HCl buffer, pH 7.4, at 37C
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.022
Clostridium histolyticum
commercial enzyme preparation, using acyl-L-lysine-4-nitroanilide as substrate, in 50 mM Tris-HCl, 5 mM dithiothreitol, and 5 mM CaCl2, at pH 7.5 and 25C
0.039
Clostridium histolyticum
purified recombinant enzyme, using acyl-L-lysine-4-nitroanilide as substrate, in 50 mM Tris-HCl, 5 mM dithiothreitol, and 5 mM CaCl2, at pH 7.5 and 25C
0.119
Clostridium histolyticum
culture supernatant, using Nalpha-benzoyl-L-arginine-4-nitroanilide as substrate, in 50 mM Tris-HCl, 5 mM dithiothreitol, and 5 mM CaCl2, at pH 7.5 and 25C
0.172
-
substrate benzoyl-L-Lys-4-nitroanilide
4.28
-
substrate benzoyl-L-Arg-4-nitroanilide
25.45
-
substrate Ac-L-Lys-4-nitroanilide
61.2
Clostridium histolyticum
recombinant enzyme after 515fold purification, using Nalpha-benzoyl-L-arginine-4-nitroanilide as substrate, in 50 mM Tris-HCl, 5 mM dithiothreitol, and 5 mM CaCl2, at pH 7.5 and 25C
66
Clostridium histolyticum
-
-
123.5
-
substrate benzoyl-L-Arg-4-nitroanilide
625.5
Clostridium histolyticum
-
-
additional information
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.5
-
substrates: Bz-L-Arg-p-nitroanilide and Ac-L-Lys-4-nitroanilide
7 - 7.2
Clostridium histolyticum
-
hydrolysis of alpha-N-benzoyl-Arg ethyl ester
7
Clostridium histolyticum
-
hydrolysis of alpha-benzoyl-Arg ethyl ester
7.2
Clostridium histolyticum
-
hydrolysis of L-Arg methyl ester, in phosphate buffer
7.3
Clostridium histolyticum
-
synthesis of carbobenzoxy-Arg-Gly-NH2
7.4 - 7.8
7.5
Clostridium histolyticum
-
synthesis of carbobenzoxy-Arg-Pro-NH2
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6 - 8
Clostridium histolyticum
-
pH 6.0: about 35% of maximal activity, pH 8.5: about 70% of maximal activity, synthesis of carbobenzoxy-Arg-Gly-NH2
6.5 - 8.5
Clostridium histolyticum
-
about 35% of maximal activity at pH 6.5 and at pH 8.5, hydrolysis of benzoyl-Arg ethyl ester
7 - 8.5
Clostridium histolyticum
-
pH 7.0: about 40% of maximal activity, pH 8.5: about 50% of maximal activity, synthesis of carbobenzoxy-Arg-Pro-NH2
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
38000
Clostridium histolyticum
-
gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
heterodimer
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
proteolytic modification
Clostridium histolyticum
-
the clostripain core protein is composed of the light and the heavy chain subunits linked by a nonapeptide into a single polypeptide chain. Linker removal is due to autocatalytic processing yielding active heterodimeric enzyme. The linker nonapeptide serves as an important transient intramolecular inhibitor in the cellular self-defense program evolved by the natural host Clostridium histolyticum
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
Clostridium histolyticum
-
Ca2+ depresses thermal inactivation
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
Ca2+ stabilizes
Clostridium histolyticum
-
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
-
Clostridium histolyticum
-
ammonium sulfate precipitation and hydrophobic interaction chromatography
Clostridium histolyticum
-
ammonium sulfate precipitation, hydroxyapatite column chromatography, and benzamidine-Sepharose column chromatography
Clostridium histolyticum
cation-exchange chromatography (SP-Sepharose), ultrafiltration
-
recombinant enzyme
Clostridium histolyticum
-
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
clostripain gene is modified and its signal sequence is replaced with that of penicillin G acylase. The core clostripain protein fused to the penicillin G acylase signal peptide is also prepared. With regard to the expression of the clostripain precursors, the majority of clostripain activity is observed in the culture media, thereby indicating that both the clostripain signal peptide and the penicillin G acylase signal peptide are recognized in the Escherichia coli secretion pathway, and the precursors successfully mature into the active form. The activity is rather low when the core protein is expressed, which indicates that the clostripain pro-peptide is important in the formation of the active enzyme in Escherichia coli
-
expressed in Clostridium perfringens strains 13 and 13DELTA6
Clostridium histolyticum
expression in Bacillus subtilis, the Clostridium histolyticum signal peptide is efficiently recognized by the Bacillus subtilis secretion apparatus; expression in Escherichia coli; the enzyme is not transported to the Escherichia coli periplasm
Clostridium histolyticum
-
expression in Escherichia coli
Clostridium histolyticum
-
overexpressed in Clostridium perfringens strain 13
-
wild-type and mutant enzyme H176A expressed in Escherichia coli
Clostridium histolyticum
-
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
C231S
Clostridium histolyticum
-
heterodimer formation is largely impaired
H176A
Clostridium histolyticum
-
no activity against N-benzoyl-L-Arg ethyl ester
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
medicine
Clostridium histolyticum
-
influence of protease inhibitors aprotinin, phenylmethylsulfonyl fluoride, L-1-chloro-3-[4-tosylamido]-7-amino-2-heptanone, and chymostatin on toxicity of Clostridium histolyticum supernatant towards HeLa cells. Aprotinin has no effect, while the other inhibitors potentiate the cytotoxic activity of Clostridium histolyticum probybly by hindering the natural defense of cells. In addition, phenylmethylsulfonyl fluoride and L-1-chloro-3-[4-tosylamido]-7-amino-2-heptanone block clostripain enzymic activity
synthesis