Any feedback?
Please rate this page
(all_enzymes.php)
(0/150)

BRENDA support

3.4.22.31: Ananain

This is an abbreviated version!
For detailed information about Ananain, go to the full flat file.

Word Map on EC 3.4.22.31

Reaction

hydrolysis of proteins with broad specificity for peptide bonds. Best reported small molecule substrate Bz-Phe-Val-Arg-/-NHMec, but broader specificity than fruit bromelain =

Synonyms

bromelain protease F9

ECTree

     3 Hydrolases
         3.4 Acting on peptide bonds (peptidases)
             3.4.22 Cysteine endopeptidases
                3.4.22.31 Ananain

Substrates Products

Substrates Products on EC 3.4.22.31 - Ananain

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
ALK + H2O
?
show the reaction diagram
-
-
-
-
?
ALR + H2O
?
show the reaction diagram
-
-
-
-
?
azocasein + H2O
fragments of azocasein
show the reaction diagram
Azocoll + H2O
Hydrolyzed azocoll
show the reaction diagram
benzoyl-Phe-Arg-7-amido-4-methylcoumarin + H2O
benzoyl-Phe-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
-
-
?
Benzoyl-Phe-Val-Arg 4-methylcoumarin 7-amide + H2O
Benzoyl-Phe-Val-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
Benzoyl-Phe-Val-Arg 4-nitroanilide + H2O
?
show the reaction diagram
-
-
-
-
?
Benzyloxycarbonyl-Arg-Arg 4-methylcoumarin 7-amide + H2O
Benzyloxycarbonyl-Arg-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
Benzyloxycarbonyl-Arg-Arg 4-nitroanilide + H2O
?
show the reaction diagram
-
-
-
-
?
benzyloxycarbonyl-Arg-Arg-7-amido-4-methylcoumarin + H2O
benzyloxycarbonyl-Arg-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
-
-
?
Benzyloxycarbonyl-Gly-Phe-citrulline 4-methylcoumarin 7-amide + H2O
Benzyloxycarbonyl-Gly-Phe-citrulline + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
-
?
Benzyloxycarbonyl-Phe-Arg 4-methylcoumarin 7-amide + H2O
Benzyloxycarbonyl-Phe-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin + H2O
benzyloxycarbonyl-Phe-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
-
-
?
Benzyloxycarbonyl-Phe-citrulline 4-methylcoumarin 7-amide + H2O
Benzyloxycarbonyl-Phe-citrulline + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
-
?
benzyloxycarbonyl-Phe-Val-Arg-7-amido-4-methylcoumarin + H2O
benzyloxycarbonyl-Phe-Val-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
-
-
?
Fibrin + H2O
Hydrolyzed fibrin
show the reaction diagram
Hemoglobin + H2O
Hydrolyzed hemoglobin
show the reaction diagram
-
-
-
?
hide powder azure + H2O
hydrolyzed hide powder azure
show the reaction diagram
Phosphorylase a + H2O
Hydrolyzed phosphorylase a
show the reaction diagram
-
-
-
?
PLK + H2O
?
show the reaction diagram
-
-
-
-
?
PLN + H2O
?
show the reaction diagram
-
-
-
-
?
PLQ + H2O
?
show the reaction diagram
-
-
-
-
?
PLR + H2O
?
show the reaction diagram
-
-
-
-
?
protein + H2O
peptides
show the reaction diagram
pyr-Glu-Phe-Leu-4-nitroanilide + H2O
pyr-Glu-Phe-Leu + 4-nitroaniline
show the reaction diagram
-
-
?
VLK + H2O
?
show the reaction diagram
-
-
-
-
?
VLR + H2O
?
show the reaction diagram
-
-
-
-
?
additional information
?
-
-
peptidyl substrate specificity analysis of ananain, detailed overview. The optimal tripeptide is PLQ with cleavage occurring after the Gln residue. Fluorescent enzyme assay method. The PLQ and VLR substrates are found to be the optimal substrates for cleavage by ananain, with a similar kcat/KM value. Substituting the P1-Arg residue of PLR, VLR, and ALR with a Lys only slightly lowers their kcat/KM values, whilst replacing the P1-Gln residue of PLQ with an Asn causes a 20fold decrease in the kcat/KM value
-
-
?