Information on EC 3.4.22.31 - Ananain

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The expected taxonomic range for this enzyme is: Ananas comosus

EC NUMBER
COMMENTARY hide
3.4.22.31
-
RECOMMENDED NAME
GeneOntology No.
Ananain
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
hydrolysis of proteins with broad specificity for peptide bonds. Best reported small molecule substrate Bz-Phe-Val-Arg-/-NHMec, but broader specificity than fruit bromelain
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of peptide bond
CAS REGISTRY NUMBER
COMMENTARY hide
119129-70-3
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
-
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Automatic Mining of ENzyme DAta
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-
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Automatic Mining of ENzyme DAta
plant
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-
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Automatic Mining of ENzyme DAta
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
azocasein + H2O
fragments of azocasein
show the reaction diagram
Azocoll + H2O
Hydrolyzed azocoll
show the reaction diagram
Benzoyl-Phe-Val-Arg 4-methylcoumarin 7-amide + H2O
Benzoyl-Phe-Val-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
Benzoyl-Phe-Val-Arg 4-nitroanilide + H2O
?
show the reaction diagram
-
-
-
-
-
Benzyloxycarbonyl-Arg-Arg 4-methylcoumarin 7-amide + H2O
Benzyloxycarbonyl-Arg-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
Benzyloxycarbonyl-Arg-Arg 4-nitroanilide + H2O
?
show the reaction diagram
-
-
-
-
-
Benzyloxycarbonyl-Gly-Phe-citrulline 4-methylcoumarin 7-amide + H2O
Benzyloxycarbonyl-Gly-Phe-citrulline + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
-
-
Benzyloxycarbonyl-Phe-Arg 4-methylcoumarin 7-amide + H2O
Benzyloxycarbonyl-Phe-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
Benzyloxycarbonyl-Phe-citrulline 4-methylcoumarin 7-amide + H2O
Benzyloxycarbonyl-Phe-citrulline + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
-
-
Fibrin + H2O
Hydrolyzed fibrin
show the reaction diagram
Hemoglobin + H2O
Hydrolyzed hemoglobin
show the reaction diagram
-
-
-
-
hide powder azure + H2O
hydrolyzed hide powder azure
show the reaction diagram
Phosphorylase a + H2O
Hydrolyzed phosphorylase a
show the reaction diagram
-
-
-
-
protein + H2O
peptides
show the reaction diagram
pyr-Glu-Phe-Leu-4-nitroanilide + H2O
pyr-Glu-Phe-Leu + 4-nitroaniline
show the reaction diagram
-
-
?
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
protein + H2O
peptides
show the reaction diagram
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
chicken cystatin
-
strong
-
Cystatin
from chicken
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L-3-carboxy-2,3-trans-epoxypropionylleucylamido(4-guanidino)butane
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i.e. E-64
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
activation of the recombinant proenzyme by incubation for 30-60 min in 50 mM sodium acetate, pH 4.0, 20 mM cysteine and 4 mM EDTA at 55°C
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0484
benzoyl-Phe-Arg 4-methylcoumarin 7-amide
-
-
0.0131
benzoyl-Phe-Val-Arg 4-methylcoumarin 7-amide
-
-
1.6
benzoyl-Phe-Val-Arg 4-nitroanilide
-
-
0.0445
benzyloxycarbonyl-Arg-Arg 4-methylcoumarin 7-amide
-
-
11
benzyloxycarbonyl-Arg-Arg 4-nitroanilide
-
-
0.0044
Benzyloxycarbonyl-Gly-Phe-citrulline 4-methylcoumarin 7-amide
-
-
0.0111
Benzyloxycarbonyl-Phe-citrulline 4-methylcoumarin 7-amide
-
-
additional information
additional information
kinetics
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TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
63.8
benzoyl-Phe-Val-Arg 4-methylcoumarin 7-amide
Ananas comosus
-
-
10.9
benzoyl-Phe-Val-Arg 4-nitroanilide
Ananas comosus
-
-
0.321
benzyloxycarbonyl-Arg-Arg 4-methylcoumarin 7-amide
Ananas comosus
-
-
0.13
benzyloxycarbonyl-Arg-Arg 4-nitroanilide
Ananas comosus
-
-
3.54
Benzyloxycarbonyl-Gly-Phe-citrulline 4-methylcoumarin 7-amide
Ananas comosus
-
-
21
Benzyloxycarbonyl-Phe-citrulline 4-methylcoumarin 7-amide
Ananas comosus
-
-
7
benzyloxycarbonyl-Phe-Val-Arg 4-methylcoumarin 7-amide
Ananas comosus
-
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5 - 5.5
recombinant mutant E35A
5.5 - 7
recombinant mutant E50A and recombinant wild-type enzyme
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
23420
-
Ananas comosus, electrospray mass spectrometry
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
modeling of three-dimensional structure, enzyme lacks the extensive network of acidic residues in and around the active site
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
-
no glycoprotein
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20°C, stored dry, stable
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
by reversible pegylation
-
recombinant enzyme from Escherichia coli
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
DNA sequence determination and analysis of pre-pro-ananain, overexpression of pro-enzyme and mutants E50A and E35A in Escherichia coli BL21(DE3)
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
E35A
site-directed mutagenesis, exchange of the conserved residue functional as part of the so-called electrostatic switch, mutation leads to an increased instability of the refolded recombinant pro-enzyme, different activation conditions are required, altered pH profile
E50A
site-directed mutagenesis, exchange of the conserved residue functional as part of the so-called electrostatic switch, slightly altered pH profile and slightly enhanced activity, activation similar to the wild-type
Renatured/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
refolding and activation of the overexpressed recombinant protein from inclusion bodies