3.4.22.31: Ananain
This is an abbreviated version!
For detailed information about Ananain, go to the full flat file.
Reaction
hydrolysis of proteins with broad specificity for peptide bonds. Best reported small molecule substrate Bz-Phe-Val-Arg-/-NHMec, but broader specificity than fruit bromelain
=
Synonyms
bromelain protease F9
ECTree
Purification
Purification on EC 3.4.22.31 - Ananain
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by reversible pegylation
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native enzyme from stem crude bromelain by cation exchange chromatography, gel filtration, and metal affinity chromatography
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recombinant enzyme from Escherichia coli