3.4.22.31: Ananain

This is an abbreviated version!
For detailed information about Ananain, go to the full flat file.

Word Map on EC 3.4.22.31

3.4.22.31

pineapple

comosus

ananas

papain

proteinases

cystatins

unbound

bromelia

Reaction

hydrolysis of proteins with broad specificity for peptide bonds. Best reported small molecule substrate Bz-Phe-Val-Arg-/-NHMec, but broader specificity than fruit bromelain =

Synonyms

bromelain protease F9

ECTree

3 Hydrolases

3.4 Acting on peptide bonds (peptidases)

3.4.22 Cysteine endopeptidases

3.4.22.31 Ananain

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Results	in table
2	Activating Compound
8	AA Sequence
1	CAS Registry Number
1	Cloned(Commentary)
1	Crystallization (Commentary)
3	General Information
9	Inhibitors
11	kcat/KM [mM/s]
22	KM Value [mM]
4	Molecular Weight [Da]
2	Natural Substrates/ Products (Substrates)
9	Organism
12	PDB ID
3	pH Optimum
1	pH Range
1	Posttranslational Modification
2	Protein Variants
6	Purification (Commentary)
3	Reaction
3	Reaction Type
9	Reference
1	Renatured (Commentary)
8	Source Tissue
2	Specific Activity [micromol/min/mg]
1	Storage Stability
42	Substrates and Products (Substrate)
6	Subunits
1	Synonyms
2	Temperature Optimum [°C]
21	Turnover Number [1/s]

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Reference on EC 3.4.22.31 - Ananain

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30265

Rowan, A.D.; Buttle, D.J.; Barrett, A.J.

Ananain: a novel cysteine proteinase found in pineapple stem

Arch. Biochem. Biophys.

267

262-270

1988

Ananas comosus

3196029

30266

Rowan, A.D.; Buttle, D.J.; Barrett, A.J.

The cysteine proteinases of the pineapple plant

Biochem. J.

266

869-875

1990

Ananas comosus

2327970

30267

Rowan, A.D.; Buttle, D.J.

Pineapple cysteine endopeptidases

Methods Enzymol.

244

555-568

1994

Ananas comosus

7845232

30268

Napper, A.D.; Bennett, S.P.; Borowski, M.; Holridge, M.B.; Leonard, M.J.C.; Rogers, E.E.; Duan, Y.; Laursen, R.A.; Reinhold, B.; Shames, S.L.

Purification and characterization of multiple forms of the pineapple-stem-derived cysteine proteinases ananain and comosain

Biochem. J.

301

727-735

1994

Ananas comosus

8053898

30269

Musu, T.; Brygier, J.; Vincentelli, J.; Guermant, C.; Paul, C.; B.Volant, D.; Looze, Y.

Easy purification of ananain through reversible pegylation

Int. J. Bio-Chromatogr.

17-27

1994

Ananas comosus

649650

Lee, K.L.; Albee, K.L.; Bernasconi, R.J.; Edmunds, T.

Complete amino acid sequence of ananain and a comparison with stem bromelain and other plant cysteine proteases

Biochem. J.

327

199-202

1997

Ananas comosus

649918

Carter, C.E.; Marriage, H.; Goodenough, P.W.

Mutagenesis and kinetic studies of a plant cysteine proteinase with an unusual arrangement of acidic amino acids in and around the active site

Biochemistry

11005-11013

2000

Ananas comosus (P80884), Ananas comosus

10998237

731758

Secor, E.R.; Szczepanek, S.M.; Singh, A.; Guernsey, L.; Natarajan, P.; Rezaul, K.; Han, D.K.; Thrall, R.S.; Silbart, L.K.

LC-MS/MS Identification of a Bromelain Peptide Biomarker from Ananas comosus Merr

Evid. Based. Complement Alternat. Med.

2012

548486

2012

Ananas comosus

23082082

752864

Yongqing, T.; Wilmann, P.G.; Pan, J.; West, M.L.; Brown, T.J.; Mynott, T.; Pike, R.N.; Wijeyewickrema, L.C.

Determination of the crystal structure and substrate specificity of ananain

Biochimie

166

194-202

2019

Ananas comosus

31306685