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Literature summary for 3.4.22.31 extracted from

  • Rowan, A.D.; Buttle, D.J.; Barrett, A.J.
    Ananain: a novel cysteine proteinase found in pineapple stem (1988), Arch. Biochem. Biophys., 267, 262-270.
    View publication on PubMed
Search for more literature for 3.4.22.31

Inhibitors

Inhibitors Comment Organism Structure
chicken cystatin strong Ananas comosus
L-3-carboxy-2,3-trans-epoxypropionylleucylamido(4-guanidino)butane i.e. E-64 Ananas comosus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0484
-
 benzoyl-Phe-Arg-7-amido-4-methylcoumarin
-
 Ananas comosus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
25000
-
 x * 25000, Ananas comosus, SDS-PAGE Ananas comosus

Organism

Organism UniProt Comment Textmining
Ananas comosus
-
 commercial preparation
-

Purification (Commentary)

Purification (Comment) Organism
-
 Ananas comosus

Source Tissue

Source Tissue Comment Organism Textmining
stem
-
 Ananas comosus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Ananas comosus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
azocasein + H2O
-
 Ananas comosus fragments of azocasein
-
 ?
Azocoll + H2O
-
 Ananas comosus Hydrolyzed azocoll
-
 ?
benzoyl-Phe-Arg-7-amido-4-methylcoumarin + H2O
-
 Ananas comosus benzoyl-Phe-Arg + 7-amino-4-methylcoumarin
-
 ?
Benzyloxycarbonyl-Arg-Arg 4-methylcoumarin 7-amide + H2O
-
 Ananas comosus Benzyloxycarbonyl-Arg-Arg + 7-amino-4-methylcoumarin
-
 ?
Benzyloxycarbonyl-Phe-Arg 4-methylcoumarin 7-amide + H2O
-
 Ananas comosus Benzyloxycarbonyl-Phe-Arg + 7-amino-4-methylcoumarin
-
 ?
benzyloxycarbonyl-Phe-Val-Arg-7-amido-4-methylcoumarin + H2O
-
 Ananas comosus benzyloxycarbonyl-Phe-Val-Arg + 7-amino-4-methylcoumarin
-
 ?
Fibrin + H2O
-
 Ananas comosus Hydrolyzed fibrin
-
 ?
hide powder azure + H2O
-
 Ananas comosus hydrolyzed hide powder azure
-
 ?
Phosphorylase a + H2O
-
 Ananas comosus Hydrolyzed phosphorylase a
-
 ?

Subunits

Subunits Comment Organism
? x * 25000, Ananas comosus, SDS-PAGE Ananas comosus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.321
-
 benzyloxycarbonyl-Arg-Arg 4-methylcoumarin 7-amide
-
 Ananas comosus
7
-
 benzyloxycarbonyl-Phe-Val-Arg-7-amido-4-methylcoumarin
-
 Ananas comosus