3.4.22.31: Ananain
This is an abbreviated version!
For detailed information about Ananain, go to the full flat file.
Reaction
hydrolysis of proteins with broad specificity for peptide bonds. Best reported small molecule substrate Bz-Phe-Val-Arg-/-NHMec, but broader specificity than fruit bromelain =
Synonyms
bromelain protease F9
ECTree
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Engineering
Engineering on EC 3.4.22.31 - Ananain
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E35A
site-directed mutagenesis, exchange of the conserved residue functional as part of the so-called electrostatic switch, mutation leads to an increased instability of the refolded recombinant pro-enzyme, different activation conditions are required, altered pH profile
E50A
site-directed mutagenesis, exchange of the conserved residue functional as part of the so-called electrostatic switch, slightly altered pH profile and slightly enhanced activity, activation similar to the wild-type