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3.4.21.63: Oryzin

This is an abbreviated version!
For detailed information about Oryzin, go to the full flat file.

Word Map on EC 3.4.21.63

Reaction

Hydrolysis of proteins with broad specificity, and of Bz-Arg-OEt > Ac-Tyr-OEt. Does not hydrolyse peptide amides =

Synonyms

Alkaline protease, Alkaline proteinase, alkaline serine protease, ALP, ALP1, AOP, AP15, AP30, API 21, Aspergillopepsin B, Aspergillopepsin F, Aspergillopeptidase B, Aspergillus alkaline proteinase, Aspergillus candidus alkaline proteinase, Aspergillus flavus alkaline proteinase, Aspergillus melleus semi-alkaline proteinase, Aspergillus oryzae alkaline proteinase, Aspergillus oryzae protease, Aspergillus parasiticus alkaline proteinase, Aspergillus serine proteinase, Aspergillus terreus protease, collagenolytic serine peptidase, EC 3.4.21.14, EC 3.4.21.15, EC 3.4.4.16, elastase, Elastinolytic serine proteinase, Kyorinase, neutral protease I, NpI, Onoprose, Onoprose SA, P 5380, Promelase, protease Alp1, Protease P, Proteinase, Aspergillus alkaline, Proteinase, Aspergillus flavus alkaline, Proteinase, Aspergillus melleus alkaline, Proteinase, Aspergillus oryzae alkaline, Proteinase, Aspergillus parasiticus alkaline, Proteinase, Aspergillus soya alkaline, Proteinase, Aspergillus sulphureus alkaline, Proteinase, Aspergillus sydowi alkaline, Prozyme, Prozyme 10, PrtA, PrtA protease, SapHM, Seaprose S, Semi-alkaline protease, Sumizyme MP

ECTree

     3 Hydrolases
         3.4 Acting on peptide bonds (peptidases)
             3.4.21 Serine endopeptidases
                3.4.21.63 Oryzin

Temperature Stability

Temperature Stability on EC 3.4.21.63 - Oryzin

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TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0 - 40
purified recombinant enzyme, 2.5 h, completely stable at
20 - 80
-
purified native extracellular enzyme, over 50% activity remaining after 15-30 min. After 60-min incubation, the enzyme preserves 70.74%, 54.22%, 94.82%, and 98.47% of its activity at 40°C, 50°C, 60°C, and 70°C, respectively
30 - 40
-
partially purified enzyme, stable at, retention of 96% activity after 1 h of incubation at 40°C
35
-
pH 7.5, highest stability
40 - 50
-
purified enzyme, 24 h, completely stable at
50 - 55
-
activity is reduced by 15% at 50°C and by 66% at 55°C
50 - 80
-
when temperature is raised from 50°C to 80°C in activity assays, the specific rate constant of crude proteolytic extract thermoinactivation increases from 0.0072 to 0.0378 per min, while that of purified protease from 0.0099 to 0.0235 per min. These values, corresponding to half-life decreases from 96.3 to 18.3 min and from 70.0 to 29.5 min, respectively, allow to estimate the activation energy, enthalpy, entropy, and Gibbs free energy of thermoinactivation, overview
60 - 70
-
purified native extracellular enzyme, 1 h, 90% activity remaining
60 - 90
-
purified secreted enzyme, 1 h, completely stable at
65
-
purified enzyme, loss of 60% activity
70
purified recombinant enzyme, 30 min, inactivation
additional information