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3.4.21.63: Oryzin

This is an abbreviated version!
For detailed information about Oryzin, go to the full flat file.

Word Map on EC 3.4.21.63

Reaction

Hydrolysis of proteins with broad specificity, and of Bz-Arg-OEt > Ac-Tyr-OEt. Does not hydrolyse peptide amides =

Synonyms

Alkaline protease, Alkaline proteinase, alkaline serine protease, ALP, ALP1, AOP, AP15, AP30, API 21, Aspergillopepsin B, Aspergillopepsin F, Aspergillopeptidase B, Aspergillus alkaline proteinase, Aspergillus candidus alkaline proteinase, Aspergillus flavus alkaline proteinase, Aspergillus melleus semi-alkaline proteinase, Aspergillus oryzae alkaline proteinase, Aspergillus oryzae protease, Aspergillus parasiticus alkaline proteinase, Aspergillus serine proteinase, Aspergillus terreus protease, collagenolytic serine peptidase, EC 3.4.21.14, EC 3.4.21.15, EC 3.4.4.16, elastase, Elastinolytic serine proteinase, Kyorinase, neutral protease I, NpI, Onoprose, Onoprose SA, P 5380, Promelase, protease Alp1, Protease P, Proteinase, Aspergillus alkaline, Proteinase, Aspergillus flavus alkaline, Proteinase, Aspergillus melleus alkaline, Proteinase, Aspergillus oryzae alkaline, Proteinase, Aspergillus parasiticus alkaline, Proteinase, Aspergillus soya alkaline, Proteinase, Aspergillus sulphureus alkaline, Proteinase, Aspergillus sydowi alkaline, Prozyme, Prozyme 10, PrtA, PrtA protease, SapHM, Seaprose S, Semi-alkaline protease, Sumizyme MP

ECTree

     3 Hydrolases
         3.4 Acting on peptide bonds (peptidases)
             3.4.21 Serine endopeptidases
                3.4.21.63 Oryzin

Inhibitors

Inhibitors on EC 3.4.21.63 - Oryzin

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INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2-mercaptoethanol
-
-
2-propanol
-
inhibits at 30% v/v
4-nitrophenyl acetate
-
increasing concentrations of the ester inhibit casein hydrolysis
acetone
acetonitrile
48% inhibition at 10%
Ba2+
-
some inhibition at 10 mM
benzamidine
slightly inhibits recombinant and native enzyme
benzeneboronic acid
-
-
Benzyloxycarbonyl-Ala-Gly-PheCH2Cl
butanol
Ca2+
-
some inhibition at 10 mM
Cd2+
-
some inhibition at 1 mM
cetyltrimethyl ammonium bromide
-
-
chloroform
-
loss of 45-88.5% activity at 15-50% v/v after 24 h, loss of 31-21% activity after 1 h
DTNB
-
52-57% inhibition at 1-5 mM
ethanol
formaldehyde
-
loss of 27-13% activity at 15-50% v/v after 24 h, loss of 46-47% activity after 1 h
iodoacetamide
-
5.2% inhibition at 5 mM
iodoacetic acid
-
strong inhibition at 5 mM
Isopropanol
leupeptin
methanol
N-bromosuccinimide
n-butanol
-
inhibition of esterolytic activity
N-Chlorosuccinimide
-
-
N-diazoacetylnorleucine methyl ester
-
8.3% inhibition at 5 mM
N-ethylmaleimide
-
11.2% inhibition at 5 mM
n-Propanol
-
inhibition of esterolytic activity
N-tosyl-L-lysine chloromethyl ketone
inhibitor of recombinant and native enzyme
Na+
-
7.5% inhibition at 5 mM
NaN3
-
slightly inhibits
Pb2+
-
some inhibition at 10 mM
pepstatin
4.8% inhibition at 1 mM
Phenylmethyl sulfonylfluoride
-
complete inhibition
phenylmethylsulfonyl fluoride
Potato inhibitor
-
Sodium azide
-
22.0% inhibition at 5 mM
sodium lauryl sulfate
-
-
Sr2+
-
some inhibition at 10 mM
tosyl-L-lysylchloromethyl ketone
-
6.7% inhibition at 5 mM
TPCK
-
moderately inhibits
Triton X-100
Trypsin inhibitor
-
moderately inhibits
-
Tween 20
Tween 80
-
72% inhibition at 1-5 mM
additional information
-