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3.4.21.63: Oryzin

This is an abbreviated version!
For detailed information about Oryzin, go to the full flat file.

Word Map on EC 3.4.21.63

Reaction

Hydrolysis of proteins with broad specificity, and of Bz-Arg-OEt > Ac-Tyr-OEt. Does not hydrolyse peptide amides =

Synonyms

Alkaline protease, Alkaline proteinase, alkaline serine protease, ALP, ALP1, AOP, AP15, AP30, API 21, Aspergillopepsin B, Aspergillopepsin F, Aspergillopeptidase B, Aspergillus alkaline proteinase, Aspergillus candidus alkaline proteinase, Aspergillus flavus alkaline proteinase, Aspergillus melleus semi-alkaline proteinase, Aspergillus oryzae alkaline proteinase, Aspergillus oryzae protease, Aspergillus parasiticus alkaline proteinase, Aspergillus serine proteinase, Aspergillus terreus protease, collagenolytic serine peptidase, EC 3.4.21.14, EC 3.4.21.15, EC 3.4.4.16, elastase, Elastinolytic serine proteinase, Kyorinase, neutral protease I, NpI, Onoprose, Onoprose SA, P 5380, Promelase, protease Alp1, Protease P, Proteinase, Aspergillus alkaline, Proteinase, Aspergillus flavus alkaline, Proteinase, Aspergillus melleus alkaline, Proteinase, Aspergillus oryzae alkaline, Proteinase, Aspergillus parasiticus alkaline, Proteinase, Aspergillus soya alkaline, Proteinase, Aspergillus sulphureus alkaline, Proteinase, Aspergillus sydowi alkaline, Prozyme, Prozyme 10, PrtA, PrtA protease, SapHM, Seaprose S, Semi-alkaline protease, Sumizyme MP

ECTree

     3 Hydrolases
         3.4 Acting on peptide bonds (peptidases)
             3.4.21 Serine endopeptidases
                3.4.21.63 Oryzin

Purification

Purification on EC 3.4.21.63 - Oryzin

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
AP30 purified by cationic exchange resin followed by an affinity column, AP15 partially purified using an anion-exchange column
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by ammonium sulfate precipitation, ion exchange chromatography and gel filtration
native enzyme 1.32fold by ammonium sulfate fractionation, and anion exchange chromatography
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native enzyme 28.6fold by acetone precipitation, freezing of the precipitate for 3 h at -20°C, dissolvation, and lyophilization, followed by concanavalin A lectin affinity chromatography
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native enzyme by anion exchange chromatography
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native enzyme partially by acetone precipitation
-
native extracellular enzyme 1.38fold from strain A10 by ammonium sulfate fractionation, dialysis, and anion exchange chromatography
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native extracellular enzyme 7fold from strain M30 by ammonium sulfate fractionation, hydrophobic interaction chromatography, and anion exchange chromatography
native salt-tolerant alkaline protease 28fold from Aspergllus oryzae strain 3.042 by ammonium sulfate fractionation, cation exchange chromatography, and gel filtration
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partially purified by ion exchange chromatography and gel filtration, 30fold
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recombinant His6-tagged enzyme from Pichia pastoris strain KM71 by nickel affinity chromatography, ultrafiltration, and gel filtration
var. columnaris, partial
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