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Literature summary for 3.4.21.63 extracted from
- Oxidant and solvent stable alkaline protease from Aspergillus flavus and its characterization (2011), Afr. J. Biotechnol., 10, 8630-8640.
No PubMed abstract available
Application
| Application | Comment | Organism |
|---|---|---|
| industry | possible use of the enzyme in detergent industry and peptide synthesis due to its compatibility with several detergents, oxidants and organic solvents | Aspergillus flavus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | about 10% inhibition at 5 mM | Aspergillus flavus |  |
| Cu2+ | over 90% inhibition at 5 mM | Aspergillus flavus | |
| Hg2+ | complete inhibition at 5 mM | Aspergillus flavus | |
| Mn2+ | about 30% inhibition at 5 mM | Aspergillus flavus | |
| additional information | poor inhibition by Ca2+ at 5 mM | Aspergillus flavus | |
| Ni2+ | about 20% inhibition at 5 mM | Aspergillus flavus | |
| Zn2+ | about 20% inhibition at 5 mM | Aspergillus flavus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Aspergillus flavus | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Fe2+ | activates 1.74fold at 5 mM | Aspergillus flavus | |
| K+ | activates about 10% at 5 mM | Aspergillus flavus | |
| additional information | no effect on enzyme activity by 5 mM Pb2+. Effect of metal salts on enzyme production, overview | Aspergillus flavus | |
| Na+ | activates about 10% at 5 mM | Aspergillus flavus | |
Organic Solvent Stability
| Organic Solvent | Comment | Organism |
|---|---|---|
| Acetone | 97.1% remaining activity after 1 h at 30°C in 25% organic solvent | Aspergillus flavus |
| benzene | 89.2% remaining activity after 1 h at 30°C in 25% organic solvent | Aspergillus flavus |
| Butanol | 96.2% remaining activity after 1 h at 30°C in 25% organic solvent | Aspergillus flavus |
| chloroform | 105.3% activity after 1 h at 30°C in 25% organic solvent | Aspergillus flavus |
| diethyl ether | 89.2% remaining activity after 1 h at 30°C in 25% organic solvent | Aspergillus flavus |
| Ethanol | 83.2% remaining activity after 1 h at 30°C in 25% organic solvent | Aspergillus flavus |
| hexane | 94.3% remaining activity after 1 h at 30°C in 25% organic solvent | Aspergillus flavus |
| Methanol | 81.7% remaining activity after 1 h at 30°C in 25% organic solvent | Aspergillus flavus |
| additional information | extremely organic solvent stable serine protease. The enzyme retains 80% of its original activity in the presence of non ionic and ionic surfactants after 1 h of incubation at 30°C, overview | Aspergillus flavus |
| toluene | 91.7% remaining activity after 1 h at 30°C in 25% organic solvent | Aspergillus flavus |
| xylene | 83.6% remaining activity after 1 h at 30°C in 25% organic solvent | Aspergillus flavus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aspergillus flavus | - |
- |
- |
| Aspergillus flavus MTCC 9952 | - |
- |
- |
Oxidation Stability
| Oxidation Stability | Organism |
|---|---|
| oxidant stable serine protease. The enzyme retains 100% of its original activity with 10% H2O2 after 1 h of incubation at 30°C | Aspergillus flavus |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native enzyme partially by acetone precipitation | Aspergillus flavus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| additional information | the organism is grown under solid state fermentation. Maximum protease yield is obtained when the strain is grown under wheat bran and corn cob mixture, ratio 1:1, incubated for 48 h at pH 9.0 and temperature 37°C with 50% of initial moisture content, evaluation overview | Aspergillus flavus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| casein + H2O | artificial substrate | Aspergillus flavus | ? | - |
? | |
| casein + H2O | artificial substrate | Aspergillus flavus MTCC 9952 | ? | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
- |
Aspergillus flavus |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 55 | 100% activity at 40°C while 92% residual activity at 45°C, 74% at 50°C, and 41% 55°C, inactive above 55°C, temperature profile, overview | Aspergillus flavus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| additional information | - |
thermal stability profile, overview | Aspergillus flavus |
| 30 | 40 | partially purified enzyme, stable at, retention of 96% activity after 1 h of incubation at 40°C | Aspergillus flavus |
| 45 | - |
84% residual activity after 1 h of incubation at 45°C | Aspergillus flavus |
| 55 | - |
53% residual activity after 1 h of incubation at 55°C | Aspergillus flavus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | 12 | - |
Aspergillus flavus |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 12 | partially purified enzyme, stable at | Aspergillus flavus |
General Information
| General Information | Comment | Organism |
|---|---|---|
| additional information | the enzyme retains 80% of its original activity in the presence of non ionic and ionic surfactants after 1 h of incubation at 30°C, overview | Aspergillus flavus |
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